LOCUS       sequence-209265-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses domain 4 of anthrolysin O cholesterol sensor, has 
            N-terminal His6-tag and two point mutations, S404C and C472A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Gay A, Rye D, Radhakrishnan A
  TITLE     Switch-like responses of two cholesterol sensors do not require 
            protein oligomerization in membranes.
  JOURNAL   Biophys J. 2015 Mar 24;108(6):1459-69. doi: 
            10.1016/j.bpj.2015.02.008.
  PUBMED    25809258
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2015.02"; journalName: "Biophys J"; date: 
            "2015-03-24- 24"; volume: "108"; issue: "6"; pages: "1459-69"
COMMENT     Sequence Label: pALOD4
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             55..87
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(91..99,100..114)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(513..531)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      527..574
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(758..777)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctttaagaag gagatataca tatgcggggt tctcatcatc atcatcatca tggtatggct
       61 agcatgactg gtggacagca aatgggtcgg gatctgtacg acgatgacga taaggatccg
      121 tgcgctaaaa tgaccctgga tcactatggc gcatacgtcg ctcagtttga tgttagttgg
      181 gacgaattta ccttcgatca aaacggcaag gaagttctga cccataaaac gtgggaaggc
      241 agtggtaaag ataagaccgc gcactattcc acggttatcc cgctgccgcc gaactcaaaa
      301 aatattaaga tcgtcgcacg tgaagcgacc ggtctggcat gggaatggtg gcgtacgatt
      361 atcaacgaac agaatgtgcc gctgaccaat gaaatcaagg tctctattgg cggcaccacg
      421 ctgtatccga cggcaaccat ctcacactaa gaattcgaag cttgatccgg ctgctaacaa
      481 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
      541 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatc
      601 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
      661 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      721 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
      781 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
      841 ttccgattta gtgcttacgg cncncgaccc caaaaacttg attagggtga tggtnacgta
      901 gtgggccatc gcctgataga cggttttcgc cttgacgt
//
