LOCUS       sequence-209278-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Shen H, Campanello GC, Flicker D, Grabarek Z, Hu J, Luo C, Banerjee 
            R, Mootha VK
  TITLE     The Human Knockout Gene CLYBL Connects Itaconate to Vitamin B12.
  JOURNAL   Cell. 2017 Nov 2;171(4):771-782.e11. doi: 
            10.1016/j.cell.2017.09.051. Epub 2017 Oct 19.
  PUBMED    29056341
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.051"; journalName: "Cell"; date: "2017-11-2-
            2"; volume: "171"; issue: "4"; pages: "771-782.e11"
COMMENT     Sequence Label: HsCLYBL(aa23-340) D320N-His
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             958..975
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggccagct tagccaaccc gcgtctgggc tacagtagca gcagccacca caagtacatc
       61 cctcgtcgcg cagtgctgta tgtgccgggc aacgatgaga agaagattaa gaagattccg
      121 agcctgaacg tggattgcgc agtgctggac tgtgaggacg gcgtggcagc caacaaaaag
      181 aacgaagccc gcctgcgcat cgtgaagacc ctggaggaca tcgatctggg cccgaccgag
      241 aaatgcgtgc gtgtgaacag cgttagcagc ggtctggcag aagaggattt agagaccctg
      301 ctgcagagcc gcgttctgcc gagcagtctg atgctgccga aggtggagag cccggaagag
      361 atccagtggt tcgccgataa gttcagcttc cacctgaagg gtcgcaagct ggagcagccg
      421 atgaacctga tccctttcgt ggagacagcc atgggcctgc tgaatttcaa agccgtgtgc
      481 gaggagaccc tgaaggttgg tccgcaagtg ggcctgttcc tggacgccgt tgtgttcggc
      541 ggtgaggact tccgcgcaag cattggcgca accagcagta aggagaccct ggatatcctg
      601 tatgcccgcc agaaaatcgt ggtgatcgcc aaggccttcg gcctgcaggc catcgacctg
      661 gtgtacattg acttccgtga tggcgccggc ttactgcgtc agagccgcga aggtgcagcc
      721 atgggcttca ccggtaagca ggttatccac cctaaccaaa tcgccgtggt gcaggaacag
      781 tttagcccga gcccggagaa gatcaagtgg gccgaagagc tgatcgccgc cttcaaggag
      841 catcagcagc tgggcaaggg tgcctttacc ttccaaggca gcatgattaa catgccgctg
      901 ctgaagcagg cccagaacac cgttaccctg gccaccagca ttaaggagaa actcgagcac
      961 caccaccacc accactgaga tccggctgca gccgtg
//
