LOCUS       sequence-209281-        1113 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Shen H, Campanello GC, Flicker D, Grabarek Z, Hu J, Luo C, Banerjee 
            R, Mootha VK
  TITLE     The Human Knockout Gene CLYBL Connects Itaconate to Vitamin B12.
  JOURNAL   Cell. 2017 Nov 2;171(4):771-782.e11. doi: 
            10.1016/j.cell.2017.09.051. Epub 2017 Oct 19.
  PUBMED    29056341
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.051"; journalName: "Cell"; date: "2017-11-2-
            2"; volume: "171"; issue: "4"; pages: "771-782.e11"
COMMENT     Sequence Label: CaMCLC-His
FEATURES             Location/Qualifiers
     source          1..1113
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1075..1092
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcgtaaac tggcccataa cttttataaa ccgctggcaa ttggagcacc ggaaccgatc
       61 cgcgaactgc cggttcgtcc ggaacgtgtg gttcatttct ttccgccgca cgtggaaaaa
      121 attcgtgctc gcatcccgga agttgctaaa caggttgatg tcctgtgcgg caacctggaa
      181 gatgcaattc cgatggacgc taaagaagcg gcccgtaatg gttttatcga agtcgtgaaa
      241 gcaaccgatt tcggcgacac ggctctgtgg gtgcgcgtta acgcgctgaa cagcccgtgg
      301 gtgctggatg acattgccga aatcgttgca gctgtcggta acaaactgga tgtcattatg
      361 atcccgaaag tggaaggccc gtgggatatt cactttgttg accagtacct ggcgctgctg
      421 gaagcccgtc atcaaatcaa aaaaccgatt ctgatccacg cgctgctgga aaccgcccag
      481 ggtatggtga atctggaaga aattgcgggt gccagcccgc gtatgcacgg tttctctctg
      541 ggtccggcgg atctggcagc ctcgcgtggt atgaaaacca cgcgcgttgg cggtggccac
      601 ccgttttatg gtgtcctggc cgatccgcag gaaggccaag cagaacgtcc gttctatcag
      661 caggatctgt ggcattacac cattgcgcgt atggttgacg tggcagttgc tcacggtctg
      721 cgtgcctttt acggtccgtt cggcgatatc aaagacgaag cagcttgcga agcacagttt
      781 cgcaatgctt tcctgctggg ttgtacgggc gcatggagtc tggctccgaa ccaaattccg
      841 atcgcaaaac gtgtcttttc cccggatgtg aatgaagttc tgttcgcgaa acgcattctg
      901 gaagccatgc cggatggttc tggcgtggcg atgatcgacg gtaaaatgca ggatgacgcg
      961 acgtggaaac aagccaaagt cattgtggat ctggcgcgta tgatcgccaa gaaggacccg
     1021 gacctggcgc aggcctatgg cctgggcggt ggttcgggcg gtggctctct cgagcaccac
     1081 caccaccacc actgagatcc ggctgcagcc gtg
//
