LOCUS       sequence-209322-        3664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In situ hybridization and RNAi studies in planarians.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Accorsi A, Williams MM, Ross EJ, Robb SMC, Elliott SA, Tu KC, 
            Sanchez Alvarado A
  TITLE     Hands-On Classroom Activities for Exploring Regeneration and Stem 
            Cell Biology with Planarians
  JOURNAL   The American Biology Teacher 79(3):208-223. Mar 2017.
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "The 
            American Biology Teacher 79(3):208-223. Mar 2017."
COMMENT     Sequence Label: Pgr_opsin
FEATURES             Location/Qualifiers
     source          1..3664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        22..40
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        98..116
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1242..1258)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1242..1258)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(1316..1334)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1339..1386
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1393..1410)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1393..1409)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1402..1424)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      1473..1559
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1651..1678
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1837..1854)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2008..2596)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2088..2107)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2743..3552)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3466..3485
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3544..3563
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 caggaaacag ctatgaccat gtaatacgac tcactatagg ggatcctcaa aaaacccctc
       61 aagacccgtt tagaggcccc aaggggttat gtggaagtaa tacgactcac tatagggcga
      121 attgggtacc gggcccggcc aattctaccc gagttgtctc acgttccttt gcttttggtg
      181 ggcagcaaca aatcaaacat ggcaccttct tctctaattg cagtcggaat ttcggatgat
      241 taatagcgta aatgaacgga ttgtacatgg cagaagtctt cgcaaacagg actggcaatt
      301 cagccgtgta cggattcaag tgatcccgcc gtccagtcag ggccattagc gctataatag
      361 cgtacggtgt ccatgacaac aaatatagaa acactattat tattgaaatt tttgctgctt
      421 gaatatcggc ttttttcgcg ccggttttta tcgacgttgg attggtggca ttcattttct
      481 gggccatttt tagcatctca agttcatgta atcgtacggc ttttacaatt tgatagtagc
      541 agaaaattat aatgccgact gggaatacaa agttgcaaat gtacattcca atgttgaaaa
      601 tgacgtttcc tttgctttgg gtcagatagt caaatgtaca tgacgtttga aatccttctg
      661 ggacgtaatg accaaatccc cagaacggag gtgacgccca aacgaacgaa taaagccaca
      721 caaaaaccag cattatgatg gccttcttca tagtcagagc ccgcatggtt tggaatggtt
      781 gcgcaatcac aaagtatcga tccaaggcta tcagggccat tgtattgatg gatataaagc
      841 caaaaagacc cccgataaag ccgtaaatct cacacgttag tttgccccag gcccatcttg
      901 tattaaaact ggaaattgtc agcaatggga acccattaac tgcggaaaag gtcaaatctc
      961 caatggccag gctcatcaca aataagttcg gaggcgttcg aagactcttg gtgcttgcaa
     1021 atatatagag caccaagaga ttgcctagca ccccggatat accgactatc gatatataga
     1081 ccccaaccaa gtagtggtac acttccggga ccatgtcaaa ttgccgccag taaggatgca
     1141 cgatggactc gaattcgtga tcccagtgcc aaggtgctgt ggttgcggac acattaacgg
     1201 tggagttcgg gatggtaatg ccgctagcca tggaaccggt ggatccacta gttctagagc
     1261 ggccgccacc gcggtggagc tcgaattcat cgatgatatc agatctgccg gtctccctat
     1321 agtgagtcgt attacttcct agcataaccc cttggggcct ctaaacgggt cttgaggggt
     1381 tttttgctta agactggccg tcgttttaca acgtcgtgac tgggaaaaca tccatgctag
     1441 cgttaacgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcggaag
     1501 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
     1561 cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg gcaggacgcc
     1621 cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat ggcctttttg
     1681 cgtttctaca aactcttcct ggctagcggt acgcgtatta attgcgttgc gctcactgcc
     1741 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     1801 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     1861 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1921 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1981 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     2041 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     2101 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     2161 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     2221 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     2281 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     2341 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2401 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     2461 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2521 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2581 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2641 cgacgcgtaa ctcacgttaa gggattttgg tcatgagctt gcgccgtccc gtcaagtcag
     2701 cgtaatgctc tgccagtgtt acaaccaatt aaccaattct gattagaaaa actcatcgag
     2761 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
     2821 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
     2881 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
     2941 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
     3001 caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
     3061 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa
     3121 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
     3181 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
     3241 tgctgttttt ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
     3301 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
     3361 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
     3421 cttcccatac aagcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
     3481 atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctcgacg tttcccgttg
     3541 aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca
     3601 tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca cgggccagag
     3661 ctgc
//
