LOCUS       sequence-209337-       14723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for Tet-inducible WTIP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14723)
  AUTHORS   Ibar C, Kirichenko E, Keepers B, Enners E, Fleisch K, Irvine KD
  TITLE     Tension-dependent regulation of mammalian Hippo signaling through 
            LIMD1.
  JOURNAL   J Cell Sci. 2018 Feb 13. pii: jcs.214700. doi: 10.1242/jcs.214700.
  PUBMED    29440237
REFERENCE   2  (bases 1 to 14723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2018 Feb 13. pii: jcs.214700. doi: 10.1242/jcs.214700."
COMMENT     Sequence Label: pTRIPZ-EGFP:WTIP
FEATURES             Location/Qualifiers
     source          1..14723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    668..789
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(838..854)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(838..854)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(851..873)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    862..878
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(886..892,893..910,911..916))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    931..952
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1069..1086)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1240..1828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1320..1339)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1999..2790,2791..2859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2622..2641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2860..2964)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    3012..3146
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             3314..3947
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3994..4119
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4616..4849
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5034..5078
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5376..5493
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5558..5932)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      5929..5938
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5942..5960)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5952..5999)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    6035..6053
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6070..6088
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6106..6124
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6142..6160
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6177..6195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6213..6231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        6243..6281
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6278..6302
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(6306..6308,6309..6311,6312..7022)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6306..6308,6309..6311,6312..7022)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6351..6372)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6612..6631)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6959..6980
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     ncRNA           8429..8522
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           8548..8660
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(8755..8773)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        8786..9997
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     9834..9853
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(10010..10043)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             10050..10757
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(10758..10791)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     misc_feature    10814..11386
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10981..10998)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     11208..11227
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             11389..11991
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     11888..11908
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12007..12595
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12060..12080)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12478..12489)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12802..13035
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(13136..13153)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    13142..13366
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      13412..13840
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13499..13518)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13709..13730
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(13849..13869)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13854..14183
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      14034..14169
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     14096..14115
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
       61 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
      121 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg
      181 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
      241 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg
      301 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt
      361 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
      421 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
      481 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagca
      541 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
      601 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
      661 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
      721 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
      781 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
      841 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      901 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      961 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     1021 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     1081 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     1141 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1201 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1261 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1321 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1381 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1441 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1501 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1561 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1621 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1681 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1741 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1801 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1861 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1921 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1981 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2041 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     2101 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     2161 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     2221 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     2281 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     2341 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     2401 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     2461 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     2521 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2581 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     2641 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2701 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2761 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2821 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2881 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2941 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     3001 atcgggagat caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3061 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3121 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
     3181 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
     3241 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
     3301 caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg
     3361 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     3421 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     3481 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     3541 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     3601 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     3661 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     3721 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     3781 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     3841 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     3901 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     3961 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     4021 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     4081 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     4141 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     4201 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     4261 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     4321 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     4381 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     4441 accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg
     4501 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     4561 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     4621 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     4681 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     4741 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     4801 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     4861 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     4921 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     4981 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     5041 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     5101 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     5161 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     5221 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     5281 gtggagagag agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc
     5341 caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     5401 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     5461 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     5521 ccagtttggt tagtaccggg cccgctctag tccggaatca gtcctgctcc tcggccacga
     5581 agtgcacgca gttgccggcc gggtcgcgca gggcgaactc ccgcccccac ggctgctcgc
     5641 cgatctcggt catggccggc ccggaggcgt cccggaagtt cgtggacacg acctccgacc
     5701 actcggcgta cagctcgtcc aggccgcgca cccacaccca ggccagggtg ttgtccggca
     5761 ccacctggtc ctggaccgcg ctgatgaaca gggtcacgtc gtcccggacc acaccggcga
     5821 agtcgtcctc cacgaagtcc cgggagaacc cgagccggtc ggtccagaac tcgaccgctc
     5881 cggcgacgtc gcgcgcggtg agcaccggaa cggcactggt caacttggcc atggtggccc
     5941 tcctatagtg agtcgtatta tactatgccg atatactatg ccgatgatta attgtcaaca
     6001 cgtgctgcag gtccgaggtt ctagacgagt ttactcccta tcagtgatag agaacgatgt
     6061 cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
     6121 gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttatccc
     6181 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     6241 gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga
     6301 tcgcaatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     6361 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     6421 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     6481 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     6541 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     6601 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     6661 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     6721 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     6781 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     6841 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     6901 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     6961 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     7021 agtactcaga tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg atgcagcgct
     7081 ccagggcggg cgcggacgag gcggccctac tcctggccgg gctggccctg cgggagctgg
     7141 agcccgggtg cggctctccc ggtcgggggc ggcgggggcc gcggcctggg cctggagacg
     7201 aggcggcgcc cgcgctgggc cgcagaggga agggcagcgg cggccccgag gccggggcgg
     7261 acggactgag ccgcggggag cggggtcccc ggcgcgcggc ggttccggag ctcagcgcgc
     7321 agcctgcggg cagcccacgg gccagcctgg cggggtccga cggcggcggc ggtggcggca
     7381 gcgcccgatc cagcggcatc agcctgggct acgaccagcg ccacggcagc ccgcgctccg
     7441 gtcgctcgga cccgcgtccc ggtcccgggc cgccttcggt gggcagcgcc cgctccagcg
     7501 tttccagcct cggctcccgg ggctcggccg gcgcctacgc tgacttcctc ccgcccggcg
     7561 cctgccccgc gcccgctcgc tccccggagc ctgcggggcc ggctcccttc ccgctgcctg
     7621 cactcccgct gccccctggc cgggagggcg gcccaagcgc ggccgagcgg cggctggagg
     7681 cgctcacccg ggagctggag cgggcgctcg aggcgcgcac ggcgcgggac tacttcggca
     7741 tttgcatcaa gtgtgggctt ggcatctacg gagcccagca ggcgtgccag gcaatgggga
     7801 gtctttatca cactgactgc ttcacctgcg actcgtgtgg gagacgactc cgtgggaagg
     7861 cgttctacaa cgtgggtgag aaagtgtact gccaggagga cttcctgtac tccgggttcc
     7921 agcagacggc cgacaaatgc agcgtgtgtg gacatctcat catggaaatg atcctgcagg
     7981 ccctgggcaa gtcctaccac ccaggctgct tccggtgctc cgtgtgcaat gagtgcctgg
     8041 acggggttcc cttcaccgtg gacgtggaga acaacatcta ctgcgtgcga gactatcaca
     8101 cggtttttgc accaaaatgc gcctcctgtg cccgtcctat cctccctgca cagggctgcg
     8161 agacaaccat ccgtgtggtg tccatggaca gagactacca cgtggcatgt taccactgtg
     8221 aggactgcgg gctgcagctg agcggggagg agggacgccg ttgctatccc ctggcgggcc
     8281 acctactgtg tcgtcgttgc cacctgcggc gcctccaacc tgggcctctt ccctcaccca
     8341 ctgtgcacgt cactgagctc agtctagata actgatcata atcagccata ccacatttgt
     8401 agaggtttta cttgctttaa tagtttgttt gaatgaggct tcagtacttt acagaatcgt
     8461 tgcctgcaca tcttggaaac acttgctggg attacttctt caggttaacc caacagaagg
     8521 ctcgaggtaa ccggatcctg atcagaattc aaggggctac tttaggagca attatcttgt
     8581 ttactaaaac tgaatacctt gctatctctt tgatacattt ttacaaagct gaattaaaat
     8641 ggtataaatt aaatcacttt tttcaattgg aagactaatg cggccggcca ttactccgtc
     8701 tcgtgtcttg ttgcatatgt ctgctggttt gtttgatgtt gtttgcgggc gggccctata
     8761 gtgagtcgta ttacctagga cgcgtggcct ccgcgccggg ttttggcgcc tcccgcgggc
     8821 gcccccctcc tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat
     8881 ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc
     8941 cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc
     9001 tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga
     9061 tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta
     9121 gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac
     9181 ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg
     9241 ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc
     9301 cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg
     9361 aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg
     9421 caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg
     9481 ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt
     9541 tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt
     9601 tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg
     9661 gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc
     9721 gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata
     9781 agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct
     9841 ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc
     9901 ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc
     9961 cgctaaattc tggccgtttt tggctttttt gttagacgct agcggatcca taacttcgta
    10021 tagtatacat tatacgaagt tatgccacca tgtctaggct ggacaagagc aaagtcataa
    10081 acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg
    10141 ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc
    10201 tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg
    10261 aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc
    10321 tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg
    10381 aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac
    10441 tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc
    10501 atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga
    10561 gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg
    10621 aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg
    10681 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgat tttgaccttg
    10741 acatgctccc cgggtaaata acttcgtata gtatacatta tacgaagtta tggatccgcg
    10801 gccgcaaatt ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
    10861 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
    10921 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
    10981 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
    11041 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
    11101 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
    11161 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
    11221 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
    11281 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
    11341 ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataataccat ggccaccgag
    11401 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc
    11461 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc
    11521 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
    11581 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
    11641 gcgggggcgg tgttcgccga gatcggctcg cgcatggccg agttgagcgg ttcccggctg
    11701 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
    11761 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
    11821 gtgctccccg gagtggaggc ggccgagcgc gctggggtgc ccgccttcct ggagacctcc
    11881 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
    11941 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg agttcgcgtc
    12001 tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
    12061 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
    12121 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
    12181 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
    12241 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
    12301 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
    12361 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
    12421 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
    12481 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
    12541 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
    12601 attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
    12661 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
    12721 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
    12781 ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga caagatctgc
    12841 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
    12901 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
    12961 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
    13021 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
    13081 agaaatgaat atcagagagt gagaggcctt gacattgttt aaacccgctg atcagcctcg
    13141 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
    13201 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
    13261 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
    13321 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
    13381 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
    13441 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
    13501 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
    13561 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
    13621 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
    13681 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
    13741 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
    13801 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
    13861 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
    13921 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
    13981 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
    14041 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
    14101 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
    14161 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
    14221 atcagcacgt gatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg
    14281 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt
    14341 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
    14401 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag
    14461 tgcttgacat tggggaatta attcagcgag agcctgacct attgcatctc ccgccgtgca
    14521 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
    14581 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
    14641 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
    14701 gatccccatg tgtatcactg gca
//
