LOCUS       sequence-209339-       15014 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for Tet-inducible Ajuba expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15014)
  AUTHORS   Ibar C, Kirichenko E, Keepers B, Enners E, Fleisch K, Irvine KD
  TITLE     Tension-dependent regulation of mammalian Hippo signaling through 
            LIMD1.
  JOURNAL   J Cell Sci. 2018 Feb 13. pii: jcs.214700. doi: 10.1242/jcs.214700.
  PUBMED    29440237
REFERENCE   2  (bases 1 to 15014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2018 Feb 13. pii: jcs.214700. doi: 10.1242/jcs.214700."
COMMENT     Sequence Label: pTRIPZ-EGFP:Ajuba
FEATURES             Location/Qualifiers
     source          1..15014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    2722..2740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2793..2811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2829..2847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2864..2882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2900..2918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2930..2968
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2965..2989
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2993..2995,2996..2998,2999..3709)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2993..2995,2996..2998,2999..3709)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3038..3059)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3299..3318)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3646..3667
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     ncRNA           5407..5500
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           5526..5638
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(5733..5751)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        5764..6975
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6812..6831
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(6988..7021)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             7028..7735
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(7736..7769)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     misc_feature    7792..8364
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7959..7976)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8186..8205
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8367..8969
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     8866..8886
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8985..9573
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9038..9058)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9456..9467)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9780..10013
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10114..10131)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10120..10344
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10390..10818
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10477..10496)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10687..10708
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10827..10847)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10832..11161
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11012..11147
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11074..11093
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    12369..12490
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12539..12555)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12539..12555)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12552..12574)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12563..12579
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12587..12593,12594..12611,12612..12617))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12632..12653
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12770..12787)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12941..13529)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13021..13040)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13700..14491,14492..14560))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14323..14342
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14561..14665)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    14713..14847
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gacgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
     2761 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     2821 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
     2881 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
     2941 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg caatggtgag
     3001 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     3061 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     3121 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     3181 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     3241 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     3301 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     3361 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     3421 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     3481 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     3541 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     3601 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     3661 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt actcagatct
     3721 cgagatggag cggttaggag agaaagccag tcgcctgctg gagaagttcg gccgcagaaa
     3781 gggtgaatct agccggtctg ggtctgacgg gacccccggg ccgggcaagg ggcgcctaag
     3841 tgggttgggg ggacctagga agtcagggcc ccgaggagct actgggggac ctggggatga
     3901 gccgttggag ccggcccggg agcaaggttc cctggacgct gagcgaaatc agcgcggctc
     3961 ctttgaggcg ccgcgctacg aaggctcttt tcccgcgggg ccgccgccca cccgggcctt
     4021 gcctctacct cagtcgttgc cccccgattt tcggctggag cccacggccc cggccctcag
     4081 cccccgctct agcttcgcca gtagctcggc cagcgacgcg agcaagccgt ccagcccccg
     4141 gggcagcctg ctgctggacg gggcgggggc tggcggagct ggaggtagcc ggccctgcag
     4201 caatcgcacc agcggcatca gcatgggcta cgaccagcgc cacgggagcc ccttgccagc
     4261 ggggccgtgc ctgtttggcc cacccctggc cggagcaccg gcaggctatt ctcccggagg
     4321 ggtcccgtcc gcctacccgg agctccacgc cgccctggac cgattgtacg ctcagcggcc
     4381 cgcggggttc ggctgccagg aaagccgcca ctcgtatccc ccggccctgg gcagccctgg
     4441 agctctagcc ggggccggag tgggagcggc ggggcccttg gagagacggg gggcgcaacc
     4501 cggacgacac tctgtgaccg gctacgggga ctgcgccgtg ggcgcccggt accaggacga
     4561 gctaacagct ttgcttcgcc tgacggtggg caccggtggg cgagaagccg gagcccgcgg
     4621 agaaccctcg gggattgagc cgtcgggtct ggaggagcca ccaggtcctt tcgttccgga
     4681 ggccgcccgg gcccggatgc gggagccaga ggccagggag gactacttcg gcacctgtat
     4741 caagtgcaac aaaggcatct atgggcagag caatgcctgc caggccctgg acagcctcta
     4801 ccacacccag tgctttgttt gctgctcttg tgggcgaact ttgcgttgca aggctttcta
     4861 cagtgtcaat ggctctgtgt actgtgagga agattatctg ttttcagggt ttcaggaggc
     4921 agctgagaaa tgctgtgtct gtggtcactt gattttggag aagatcctac aagcaatggg
     4981 gaagtcctat catccaggct gtttccgatg cattgtttgc aacaagtgcc tggatggcat
     5041 ccccttcaca gtggacttct ccaaccaagt atactgtgtc accgactacc acaaaaatta
     5101 tgctcctaag tgtgcagcct gtggccaacc catcctcccc tctgagggct gtgaggacat
     5161 cgtgagggtg atatccatgg accgggatta tcactttgag tgctaccact gtgaggactg
     5221 ccggatgcag ctgagtgatg aggaaggctg ctgctgtttc cctctggatg ggcacttgct
     5281 ctgccatggt tgccacatgc agcggctcaa tgcccgacaa ccccctgcca actatatcag
     5341 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaata
     5401 gtttgtttga atgaggcttc agtactttac agaatcgttg cctgcacatc ttggaaacac
     5461 ttgctgggat tacttcttca ggttaaccca acagaaggct cgaggtaacc ggatcctgat
     5521 cagaattcaa ggggctactt taggagcaat tatcttgttt actaaaactg aataccttgc
     5581 tatctctttg atacattttt acaaagctga attaaaatgg tataaattaa atcacttttt
     5641 tcaattggaa gactaatgcg gccggccatt actccgtctc gtgtcttgtt gcatatgtct
     5701 gctggtttgt ttgatgttgt ttgcgggcgg gccctatagt gagtcgtatt acctaggacg
     5761 cgtggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg
     5821 ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga
     5881 cagcggcccg ctgctcataa gactcggcct tagaacccca gtatcagcag aaggacattt
     5941 taggacggga cttgggtgac tctagggcac tggttttctt tccagagagc ggaacaggcg
     6001 aggaaaagta gtcccttctc ggcgattctg cggagggatc tccgtggggc ggtgaacgcc
     6061 gatgattata taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg
     6121 ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg
     6181 gctggccggg gctttcgtgg ccgccgggcc gctcggtggg acggaagcgt gtggagagac
     6241 cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc tgaactgggg gttgggggga
     6301 gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg
     6361 tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca agaacccaag gtcttgaggc
     6421 cttcgctaat gcgggaaagc tcttattcgg gtgagatggg ctggggcacc atctggggac
     6481 cctgacgtga agtttgtcac tgactggaga actcggtttg tcgtctgttg cgggggcggc
     6541 agttatggcg gtgccgttgg gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt
     6601 gtcgtgacgt cacccgttct gttggcttat aatgcagggt ggggccacct gccggtaggt
     6661 gtgcggtagg cttttctccg tcgcaggacg cagggttcgg gcctagggta ggctctcctg
     6721 aatcgacagg cgccggacct ctggtgaggg gagggataag tgaggcgtca gtttctttgg
     6781 tcggttttat gtacctatct tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg
     6841 gggttggcga gtgtgttttg tgaagttttt taggcacctt ttgaaatgta atcatttggg
     6901 tcaatatgta attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg
     6961 gcttttttgt tagacgctag cggatccata acttcgtata gtatacatta tacgaagtta
     7021 tgccaccatg tctaggctgg acaagagcaa agtcataaac ggagctctgg aattactcaa
     7081 tggtgtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca
     7141 gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga
     7201 gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt
     7261 tctgcggaac aacgccaagt cataccgctg tgctctcctc tcacatcgcg acggggctaa
     7321 agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc
     7381 gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg
     7441 ccactttaca ctgggctgcg tattggagga acaggagcat caagtagcaa aagaggaaag
     7501 agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga
     7561 ccggcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga
     7621 gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga
     7681 catgctccca gccgatgccc ttgacgattt tgaccttgac atgctccccg ggtaaataac
     7741 ttcgtatagt atacattata cgaagttatg gatccgcggc cgcaaattcc gcccctctcc
     7801 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     7861 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     7921 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     7981 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     8041 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     8101 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     8161 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     8221 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     8281 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     8341 tttcctttga aaaacacgat aataccatgg ccaccgagta caagcccacg gtgcgcctcg
     8401 ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc
     8461 ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag
     8521 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     8581 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     8641 tcggctcgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     8701 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     8761 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     8821 ccgagcgcgc tggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     8881 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     8941 ggtgcatgac ccgcaagccc ggtgcctgag ttcgcgtctg gaacaatcaa cctctggatt
     9001 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     9061 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     9121 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     9181 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     9241 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     9301 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     9361 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     9421 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     9481 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     9541 cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct
     9601 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     9661 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     9721 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact
     9781 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     9841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     9901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     9961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
    10021 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
    10081 gaggccttga cattgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
    10141 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
    10201 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
    10261 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
    10321 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
    10381 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
    10441 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
    10501 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
    10561 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
    10621 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
    10681 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
    10741 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
    10801 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
    10861 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
    10921 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
    10981 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
    11041 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
    11101 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
    11161 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtga tgaaaaagcc
    11221 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
    11281 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
    11341 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
    11401 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattaat
    11461 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
    11521 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
    11581 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
    11641 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
    11701 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
    11761 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
    11821 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
    11881 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
    11941 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
    12001 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
    12061 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
    12121 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
    12181 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagc
    12241 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
    12301 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
    12361 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    12421 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    12481 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
    12541 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    12601 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    12661 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    12721 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    12781 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    12841 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    12901 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    12961 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    13021 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    13081 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    13141 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    13201 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    13261 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    13321 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    13381 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    13441 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    13501 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    13561 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    13621 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    13681 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    13741 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    13801 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    13861 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    13921 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    13981 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    14041 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    14101 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    14161 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    14221 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    14281 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    14341 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    14401 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    14461 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    14521 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    14581 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    14641 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
    14701 gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    14761 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    14821 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
    14881 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
    14941 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
    15001 acaaacttag tagt
//
