LOCUS       sequence-209340-        3702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HLA-A0201 his tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Lampen MH, Uchtenhagen H, Blom K, Varnaite R, Pakalniene J, 
            Dailidyte L, Walchli S, Lindquist L, Mickiene A, Michaelsson J, 
            Schumacher TN, Ljunggren HG, Sandberg JK, Achour A, Gredmark-Russ S
  TITLE     Breadth and Dynamics of HLA-A2- and HLA-B7-Restricted CD8(+) T Cell 
            Responses against Nonstructural Viral Proteins in Acute Human 
            Tick-Borne Encephalitis Virus Infection.
  JOURNAL   Immunohorizons. 2018 Jul 2;2(6):172-184. doi: 
            10.4049/immunohorizons.1800029.
  PUBMED    31022685
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/immunohorizons"; journalName: "Immunohorizons"; date: 
            "2018-07-2- 2"; volume: "2"; issue: "6"; pages: "172-184"
COMMENT     Sequence Label: pENTR-HLA-A0201-His
FEATURES             Location/Qualifiers
     source          1..3702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1784..1801
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(1827..1926)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1943..1962)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1944..1962)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1967..1983)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1967..1983)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2085..2104)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2096..2905
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2163..2182)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3052..3640
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3541..3560
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgccc ccttcaccat ggccgtcatg gcgccccgaa ccctcgtcct
      721 gctactctcg ggggctctgg ccctgaccca gacctgggcg ggctctcact ccatgaggta
      781 tttcttcaca tccgtgtccc ggcccggccg cggggagccc cgcttcatcg cagtgggcta
      841 cgtggacgac acgcagttcg tgcggttcga cagcgacgcc gcgagccaga ggatggagcc
      901 gcgggcgccg tggatagagc aggagggtcc ggagtattgg gacggggaga cacggaaagt
      961 gaaggcccac tcacagactc accgagtgga cctggggacc ctgcgcggct actacaacca
     1021 gagcgaggcc ggttctcaca ccgtccagag gatgtatggc tgcgacgtgg ggtcggactg
     1081 gcgcttcctc cgcgggtacc accagtacgc ctacgacggc aaggattaca tcgccctgaa
     1141 agaggacctg cgctcttgga ccgcggcgga catggcagct cagaccacca agcacaagtg
     1201 ggaggcggcc catgtggcgg agcagttgag agcctacctg gagggcacgt gcgtggagtg
     1261 gctccgcaga tacctggaga acgggaagga gacgctgcag cgcacggacg cccccaaaac
     1321 gcatatgact caccacgctg tctctgacca tgaagccacc ctgaggtgct gggccctgag
     1381 cttctaccct gcggagatca cactgacctg gcagcgggat ggggaggacc agacccagga
     1441 cacggagctc gtggagacca ggcctgcagg ggatggaacc ttccagaagt gggcggctgt
     1501 ggtggtgcct tctggacagg agcagagata cacctgccat gtgcagcatg agggtttgcc
     1561 caagcccctc accctgagat gggagccgtc ttcccagccc accatcccca tcgtgggcat
     1621 cattgctggc ctggttctct ttggagctgt gatcactgga gctgtggtcg ctgctgtgat
     1681 gtggaggagg aagagctcag atagaaaagg agggagctac tctcaggctg caagcagtga
     1741 cagtgcccag ggctctgatg tgtctctcac agcttgtaaa gtgcatcacc atcaccatca
     1801 ctgactcgag aagggtgggc gcgccgaccc agctttcttg tacaaagttg gcattataag
     1861 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     1921 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     1981 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     2041 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     2101 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
     2161 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
     2221 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     2281 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     2341 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa
     2401 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     2461 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     2521 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     2581 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     2641 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     2701 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     2761 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     2821 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     2881 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag
     2941 cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta
     3001 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3061 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3121 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3181 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3241 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3301 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3361 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3421 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3481 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3541 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3601 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3661 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
