LOCUS       sequence-209342-        7172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A somatic channelrhodopsin, which enables single cell, single 
            millisecond resolution optogenetics. CaMKII promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Shemesh OA, Tanese D, Zampini V, Linghu C, Piatkevich K, Ronzitti E,
            Papagiakoumou E, Boyden ES, Emiliani V
  TITLE     Temporally precise single-cell-resolution optogenetics.
  JOURNAL   Nat Neurosci. 2017 Dec;20(12):1796-1806. doi: 
            10.1038/s41593-017-0018-8. Epub 2017 Nov 13.
  PUBMED    29184208
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-017-0018-8"; journalName: "Nat Neurosci"; date: 
            "2017-12"; volume: "20"; issue: "12"; pages: "1796-1806"
COMMENT     Sequence Label: pAAV-CaMKII-soCoChR-GFP
FEATURES             Location/Qualifiers
     source          1..7172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   23..152
                     /label=AAV2 ITR
     promoter        225..1513
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             1529..2392
                     /codon_start=1
                     /product="high-photocurrent channelrhodopsin from 
                     Chloromonas oogama (Shemesh et al., 2017)"
                     /label=CoChR
                     /translation="MLGNGSAIVPIDQCFCLAWTDSLGSDTEQLVANILQWFAFGFSIL
                     ILMFYAYQTWRATCGWEEVYVCCVELTKVIIEFFHEFDDPSMLYLANGHRVQWLRYAEW
                     LLTCPVILIHLSNLTGLKDDYSKRTMRLLVSDVGTIVWGATSAMSTGYVKVIFFVLGCI
                     YGANTFFHAAKVYIESYHVVPKGRPRTVVRIMAWLFFLSWGMFPVLFVVGPEGFDAISV
                     YGSTIGHTIIDLMSKNCWGLLGHYLRVLIHQHIIIYGDIRKKTKINVAGEEMEVETMVD
                     QEDEETV"
     CDS             2429..2878
                     /codon_start=1
                     /product="first 150 residues of the mouse kainate receptor 
                     subunit 2"
                     /label=KA2
                     /note="mediates targeting to the neuronal soma (Shemesh et 
                     al., 2017)"
                     /translation="MPAELLLLLIVAFANPSCQVLSSLRMAAILDDQTVCGRGERLALA
                     LAREQINGIIEVPAKARVEVDIFELQRDSQYETTDTMCQILPKGVVSVLGPSSSPASAS
                     TVSHICGEKEIPHIKVGPEETPRLQYLRFASVSLYPSNEDVSLAVS"
     CDS             join(2897..2899,2900..3613)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2897..2899,2900..3610)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2939..2960)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3200..3219)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3547..3568
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3638..4226
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3691..3711)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4109..4120)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4269..4390
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4280..4299)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4334..4353
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4552..4681
                     /label=AAV2 ITR
     rep_origin      complement(4918..5506)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4998..5017)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5677..6468,6469..6537))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6300..6319
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6538..6642)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6668..7123)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6805..6826)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7017..7036
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcctcttcg ctattacgcc agctgcgcgc tcgctcgctc actgaggccg cccgggcaaa
       61 gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga
      121 gggagtggcc aactccatca ctaggggttc cttgtagtta atgattaacc cgccatgcta
      181 cttatctacg tagccatgct ctaggaagat cgtaggttaa ttaacattat ggccttaggt
      241 cacttcatct ccatggggtt cttcttctga ttttctagaa aatgagatgg gggtgcagag
      301 agcttcctca gtgacctgcc cagggtcaca tcagaaatgt cagagctaga acttgaactc
      361 agattactaa tcttaaattc catgccttgg gggcatgcaa gtacgatata cagaaggagt
      421 gaactcatta gggcagatga ccaatgagtt taggaaagaa gagtccaggg cagggtacat
      481 ctacaccacc cgcccagccc tgggtgagtc cagccacgtt cacctcatta tagttgcctc
      541 tctccagtcc taccttgacg ggaagcacaa gcagaaactg ggacaggagc cccaggagac
      601 caaatcttca tggtccctct gggaggatgg gtggggagag ctgtggcaga ggcctcagga
      661 ggggccctgc tgctcagtgg tgacagatag gggtgagaaa gcagacagag tcattccgtc
      721 agcattctgg gtctgtttgg tacttcttct cacgctaagg tggcggtgtg atatgcacaa
      781 tggctaaaaa gcagggagag ctggaaagaa acaaggacag agacagaggc caagtcaacc
      841 agaccaattc ccagaggaag caaagaaacc attacagaga ctacaagggg gaagggaagg
      901 agagatgaat tagcttcccc tgtaaacctt agaacccagc tgttgccagg gcaacggggc
      961 aatacctgtc tcttcagagg agatgaagtt gccagggtaa ctacatcctg tctttctcaa
     1021 ggaccatccc agaatgtggc acccactagc cgttaccata gcaactgcct ctttgcccca
     1081 cttaatccca tcccgtctgt taaaagggcc ctatagttgg aggtggggga ggtaggaaga
     1141 gcgatgatca cttgtggact aagtttgttc gcatcccctt ctccaacccc ctcagtacat
     1201 caccctgggg gaacagggtc cacttgctcc tgggcccaca cagtcctgca gtattgtgta
     1261 tataaggcca gggcaaagag gagcaggttt taaagtgaaa ggcaggcagg tgttggggag
     1321 gcagttaccg gggcaacggg aacagggcgt ttcggaggtg gttgccatgg ggacctggat
     1381 gctgacgaag gctcgcgagg ctgtgagcag ccacagtgcc ctgctcagaa gccccaagct
     1441 cgtcagtcaa gccggttctc cgtttgcact caggagcacg ggcaggcgag tggcccctag
     1501 ttctgggggc agcgggggat ccgccaccat gctggggaac ggcagcgcca ttgtgcctat
     1561 cgaccagtgc ttttgcctgg cttggaccga cagcctggga agcgatacag agcagctggt
     1621 ggccaacatc ctccagtggt tcgccttcgg cttcagcatc ctgatcctga tgttctacgc
     1681 ctaccagact tggagagcca cttgcggttg ggaggaggtc tacgtctgtt gcgtcgagct
     1741 gaccaaggtc atcatcgagt tcttccacga gttcgacgac cccagcatgc tgtacctggc
     1801 taacggacac cgagtccagt ggctgagata cgcagagtgg ctgctgactt gtcccgtcat
     1861 cctgatccac ctgagcaacc tgaccggcct gaaggacgac tacagcaagc ggaccatgag
     1921 gctgctggtg tcagacgtgg gaaccatcgt gtggggagct acaagcgcca tgagcacagg
     1981 ctacgtcaag gtcatcttct tcgtgctggg ttgcatctac ggcgccaaca ccttcttcca
     2041 cgccgccaag gtgtatatcg agagctacca cgtggtgcca aagggcagac ctagaaccgt
     2101 cgtgcggatc atggcttggc tgttcttcct gtcttggggc atgttccccg tgctgttcgt
     2161 cgtgggacca gaaggattcg acgccatcag cgtgtacggc tctaccattg gccacaccat
     2221 catcgacctc atgagcaaga attgttgggg cctgctggga cactatctga gagtgctgat
     2281 ccaccagcac atcatcatct acggcgacat ccggaagaag accaagatca acgtggccgg
     2341 cgaggagatg gaagtggaga ccatggtgga ccaggaggac gaggagacag tgggaggttc
     2401 aggtggaacc ggtggaagtg gaggtaccat gcccgccgaa ctgctgctgc tgctgattgt
     2461 cgcttttgct aacccttctt gccaggtgct gtcttctctg aggatggccg ctattctgga
     2521 cgatcagacc gtgtgcggaa gaggagagag gctggcactg gcactggcta gggagcagat
     2581 caatggcatc attgaagtgc ctgcaaaggc ccgggtcgag gtggacattt tcgaactgca
     2641 gagagatagc cagtacgaga ccacagacac catgtgccag atcctgccaa aaggagtggt
     2701 ctccgtcctg ggaccaagct cctctcctgc ttctgcaagt accgtgtctc acatttgtgg
     2761 cgagaaggaa atcccccata tcaaggtcgg gccagaggaa acacccaggc tgcagtacct
     2821 gcgcttcgcc tcagtgagcc tgtatccatc aaacgaggat gtgtcactgg cagtcagcgg
     2881 agctagcgga ggtactgtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     2941 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     3001 cgatgccacc tacggcaagc tgaccctgaa gttcatttgc accaccggca agctgcccgt
     3061 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     3121 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     3181 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     3241 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     3301 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     3361 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     3421 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     3481 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     3541 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     3601 gctgtacaag taagaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     3661 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     3721 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     3781 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     3841 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     3901 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     3961 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     4021 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     4081 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     4141 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     4201 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cccgggcggc cgcttcgagc
     4261 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     4321 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     4381 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
     4441 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatcttcc
     4501 tagagcatgg ctacgtagat aagtagcatg gcgggttaat cattaactac aaggaacccc
     4561 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     4621 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     4681 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     4741 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4801 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4861 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4921 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4981 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5041 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5101 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5161 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5221 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5281 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5341 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5401 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5461 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5521 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5581 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5641 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5701 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5761 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5821 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5881 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     5941 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     6001 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6061 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6121 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6181 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6241 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6301 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6361 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6421 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6481 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6541 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6601 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     6661 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     6721 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     6781 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     6841 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     6901 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     6961 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     7021 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     7081 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     7141 ctgcgcaact gttgggaagg gcgatcggtg cg
//
