LOCUS       sequence-209346-        7831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ag43 under the control of an L-arabinose transcriptional 
            regulator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7831)
  AUTHORS   Jin X, Riedel-Kruse IH
  TITLE     Biofilm Lithography enables high-resolution cell patterning via 
            optogenetic adhesin expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Apr 3;115(14):3698-3703. doi: 
            10.1073/pnas.1720676115. Epub 2018 Mar 19.
  PUBMED    29555779
REFERENCE   2  (bases 1 to 7831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1720676115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-04-3- 3"; volume: "115"; issue: "14"; pages: "3698-3703"
COMMENT     Sequence Label: pBAD-Ag43
FEATURES             Location/Qualifiers
     source          1..7831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..879)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        906..1190
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1113..1132
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             1219..1230
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      4374..4445
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4461..4488
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    4495..4515
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      4516..4573
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(4597..4614)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(4876..5826)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5874..6096
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(6588..6682)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(6712..7503,7504..7572))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7335..7354
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7573..7677)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7742..7760
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(7764..7807)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    7810..7831
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagaatg aaacgacatc tgaatacctg
     1261 ctacaggctg gtatggaatc acatgacggg cgctttcgtg gttgcctccg aactggcccg
     1321 cgcacggggt aaacgtggcg gtgtggcggt tgcactgtct cttgccgcag tcacgtcact
     1381 cccggtgctg gctgctgaca tcgttgtgca cccgggagaa accgtgaacg gcggaacact
     1441 ggcaaatcat gacaaccaga ttgtcttcgg tacgaccaac ggaatgacca tcagtaccgg
     1501 gctggagtat gggccggata acgaggccaa taccggcggg caatgggtac aggatggcgg
     1561 aacagccaac aaaacgactg tcaccagtgg tggtcttcag agagtgaacc ccggtggaag
     1621 tgtctcagac acggttatca gtgccggagg cggacagagc cttcagggac gggctgtgaa
     1681 caccacgctg aatggtggcg aacagtggat gcatgagggg gcgatagcca caggaaccgt
     1741 cattaatgat aagggctggc aggtcgtcaa gcccggtaca gtggcaacgg ataccgttgt
     1801 taataccggg gcggaagggg gaccggatgc agaaaacggt gataccgggc agtttgttcg
     1861 cggggatgcc gtacgcacaa ccatcaataa aaacggtcgc cagattgtga gagctgaagg
     1921 aacggcaaat accactgtgg tttatgccgg cggcgaccag actgtacatg gtcacgcact
     1981 ggataccacg ctgaatgggg gataccagta tgtgcacaac ggcggtacag cgtctgacac
     2041 tgttgtgaac agtgacggct ggcagattgt caaaaacggg ggtgtggccg ggaataccac
     2101 cgttaatcag aagggcagac tgcaagtgga cgccggtggt acagccacga atgtcaccct
     2161 gaagcagggc ggcgcactgg ttaccagtac ggctgcaacc gttaccggca taaaccgcct
     2221 gggagcattc tctgttgtgg agggtaaagc tgataatgtc gtactggaaa atggcggacg
     2281 cctggatgtg ctgaccggac acacagccac taatacccgc gtggatgatg gcggaacgct
     2341 ggatgtccgc aacggtggca ccgccaccac cgtatccatg ggaaatggcg gtgtactgct
     2401 ggccgattcc ggtgccgctg tcagtggtac ccggagcgac ggaaaggcat tcagtatcgg
     2461 aggcggtcag gcggatgccc tgatgctgga aaaaggcagt tcattcacgc tgaacgccgg
     2521 tgatacggcc acggatacca cggtaaatgg cggactgttc accgccaggg gcggcacact
     2581 ggcgggcacc accacgctga ataacggcgc catacttacc ctttccggga agacggtgaa
     2641 caacgatacc ctgaccatcc gtgaaggcga tgcactcctg caaggaggct ctctcaccgg
     2701 taacggcagc gtggaaaaat caggaagtgg cacactcact gtcagcaaca ccacactcac
     2761 ccagaaagcc gtcaacctga atgaaggcac gctgacgctg aacgacagta ccgtcaccac
     2821 ggatgtcatt gctcagcgcg gtacagccct gaagctgacc ggcagcactg tgctgaacgg
     2881 tgccattgac cccacgaatg tcactctcgc ctccggtgcc acctggaata tccccgataa
     2941 cgccacggtg cagtcggtgg tggatgacct cagccatgcc ggacagattc atttcacctc
     3001 cacccgcaca gggaagttcg taccggcaac cctgaaagtg aaaaacctga acggacagaa
     3061 tggcaccatc agcctgcgtg tacgcccgga tatggcacag aacaatgctg acagactggt
     3121 cattgacggc ggcagggcaa ccggaaaaac catcctgaac ctggtgaacg ccggcaacag
     3181 tgcgtcgggg ctggcgacca gcggtaaggg tattcaggtg gtggaagcca ttaacggtgc
     3241 caccacggag gaaggggcct ttgtccaggg gaacaggctg caagccggtg cctttaacta
     3301 ctccctcaac cgggacagtg atgagagctg gtatctgcgc agtgaaaatg cttatcgtgc
     3361 agaagtcccc ctgtatgcct ccatgctgac acaggcaatg gactatgacc ggattgtggc
     3421 aggctcccgc agccatcaga ccggtgtaaa tggtgaaaac aacagcgtcc gtctcagcat
     3481 tcagggcggt catctcggtc acgataacaa tggcggtatt gcccgtgggg ccacgccgga
     3541 aagcagcggc agctatggat tcgtccgtct ggagggtgac ctgatgagaa cagaggttgc
     3601 cggtatgtct gtgaccgcgg gggtatatgg tgctgctggc cattcttccg ttgatgttaa
     3661 ggatgatgac ggctcccgtg ccggcacggt ccgggatgat gccggcagcc tgggcggata
     3721 cctgaatctg gtacacacgt cctccggcct gtgggctgac attgtggcac agggaacccg
     3781 ccacagcatg aaagcgtcat cggacaataa cgacttccgc gcccggggct ggggctggct
     3841 gggctcactg gaaaccggtc tgcccttcag tatcactgac aacctgatgc tggagccaca
     3901 actgcaatat acctggcagg gactttccct ggatgacggt aaggacaacg ccggttatgt
     3961 gaagttcggg catggcagtg cacaacatgt gcgtgccggt ttccgtctgg gcagccacaa
     4021 cgatatgacc tttggcgaag gcacctcatc ccgtgccccc ctgcgtgaca gtgcaaaaca
     4081 cagtgtgagt gaattaccgg tgaactggtg ggtacagcct tctgttatcc gcaccttcag
     4141 ctcccgggga gatatgcgtg tggggacttc cactgcaagc agcgggatga cgttctctcc
     4201 ctcacagaat ggcacatcac tggacctgca agccggactg gaagcccgtg tccgggaaaa
     4261 tatcaccctg ggcgttcagg ccggttatgc ccacagcgtc agcggcagca gcgctgaagg
     4321 gtataacggt caggccacac tgaatgtgac cttctgatac tagagccagg catcaaataa
     4381 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     4441 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
     4501 tagcggccgc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt
     4561 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
     4621 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatct cgaggttaca
     4681 ttgtcgatct gttcatggtg aacagctttg aatgcaccaa aaactcgtaa aagctctgat
     4741 gtatctatct tttttacacc gttttcatct gtgcatatgg acagttttcc ctttgatatg
     4801 taacggtgaa cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca
     4861 agagccataa gaacctcaga tccttccgta tttagccagt atgttctcta gtgtggttcg
     4921 ttgtttttgc gtgagccatg agaacgaacc attgagatca tacttacttt gcatgtcact
     4981 caaaaatttt gcctcaaaac tggtgagctg aatttttgca gttaaagcat cgtgtagtgt
     5041 ttttcttagt ccgttatgta ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc
     5101 attcattttt atctggttgt tctcaagttc ggttacgaga tccatttgtc tatctagttc
     5161 aacttggaaa atcaacgtat cagtcgggcg gcctcgctta tcaaccacca atttcatatt
     5221 gctgtaagtg tttaaatctt tacttattgg tttcaaaacc cattggttaa gccttttaaa
     5281 ctcatggtag ttattttcaa gcattaacat gaacttaaat tcatcaaggc taatctctat
     5341 atttgccttg tgagttttct tttgtgttag ttcttttaat aaccactcat aaatcctcat
     5401 agagtatttg ttttcaaaag acttaacatg ttccagatta tattttatga atttttttaa
     5461 ctggaaaaga taaggcaata tctcttcact aaaaactaat tctaattttt cgcttgagaa
     5521 cttggcatag tttgtccact ggaaaatctc aaagccttta accaaaggat tcctgatttc
     5581 cacagttctc gtcatcagct ctctggttgc tttagctaat acaccataag cattttccct
     5641 actgatgttc atcatctgag cgtattggtt ataagtgaac gataccgtcc gttctttcct
     5701 tgtagggttt tcaatcgtgg ggttgagtag tgccacacag cataaaatta gcttggtttc
     5761 atgctccgtt aagtcatagc gactaatcgc tagttcattt gctttgaaaa caactaattc
     5821 agacatacat ctcaattggt ctaggtgatt ttaatcacta taccaattga gatgggctag
     5881 tcaatgataa ttacatgtcc ttttcctttg agttgtgggt atctgtaaat tctgctagac
     5941 ctttgctgga aaacttgtaa attctgctag accctctgta aattccgcta gacctttgtg
     6001 tgtttttttt gtttatattc aagtggttat aatttataga ataaagaaag aataaaaaaa
     6061 gataaaaaga atagatccca gccctgtgta taactcacta ctttagtcag ttccgcagta
     6121 ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt aaaaccctaa
     6181 aggcttaagt agcaccctcg caagctcggg caaatcgctg aatattcctt ttgtctccga
     6241 ccatcaggca cctgagtcgc tgtctttttc gtgacattca gttcgctgcg ctcacggctc
     6301 tggcagtgaa tgggggtaaa tggcactaca ggcgcctttt atggattcat gcaaggaaac
     6361 tacccataat acaagaaaag cccgtcacgg gcttctcagg gcgttttatg gcgggtctgc
     6421 tatgtggtgc tatctgactt tttgctgttc agcagttcct gccctctgat tttccagtct
     6481 gaccacttcg gattatcccg tgacaggtca ttcagactgg ctaatgcacc cagtaaggca
     6541 gcggtatcat caacaggctt acccgtctta ctgtccctag tgcttggatt ctcaccaata
     6601 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt
     6661 actggatcta tcaacaggag tccaagcgag ctcgtaaact tggtctgaca gttaccaatg
     6721 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6781 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     6841 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     6901 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6961 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     7021 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     7081 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7141 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7201 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7261 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7321 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7381 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7441 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7501 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7561 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7621 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7681 atttcccatg gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     7741 aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga
     7801 tgatttctgg aattcgcggc cgcttctaga g
//
