LOCUS       sequence-209364-        3154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phagemid for producing DNA origami scaffolds, derived from pUC18 and
            M13mp18.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM
  TITLE     Construction of a novel phagemid to produce custom DNA origami 
            scaffolds.
  JOURNAL   Synth Biol (Oxf). 2018 Jan;3(1). doi: 10.1093/synbio/ysy015. Epub 
            2018 Aug 9.
  PUBMED    30984875
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2018-01"; volume: "3(1). doi"; pages: " 
            10.1093/synbio/ysy015. Epub 2018 Aug 9"
COMMENT     Sequence Label: pScaf
FEATURES             Location/Qualifiers
     source          1..3154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..25
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      44..424
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(788..804)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(788..804)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(801..823)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    812..828
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(836..842,843..860,861..866))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    881..902
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1019..1036)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1190..1778)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1270..1289)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1949..2740,2741..2809))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2572..2591
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2810..2914)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2982..3000
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3038..3060)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgacgcgcc ctgtagcggc
       61 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      121 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      181 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      241 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      301 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
      361 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
      421 tcggggtacc aaaaaaggat ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      481 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
      541 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
      601 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
      661 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
      721 gagtccacgt tctttaatag tggactcttg ttccaaactg gtttaacact cagaattcgt
      781 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
      841 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
      901 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      961 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     1021 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     1081 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     1141 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1201 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     1261 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     1321 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1381 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1441 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1501 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1561 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1621 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     1681 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1741 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1801 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     1861 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     1921 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     1981 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     2041 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     2101 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     2161 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     2221 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     2281 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     2341 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     2401 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     2461 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     2521 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     2581 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     2641 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     2701 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     2761 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     2821 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     2881 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     2941 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     3001 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     3061 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     3121 cagcgggtgt tggcgggtgt cggggctggc ttaa
//
