LOCUS       sequence-209370-       10465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses polyubiquitin GCaMP6s, a genetically encoded calcium 
            indicator, for live calcium imaging in Aedes aegypti mosquitoes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Bui M, Shyong J, Lutz EK, Yang T, Li M, Truong K, Arvidson R, 
            Buchman A, Riffell JA, Akbari OS
  TITLE     Live calcium imaging of Aedes aegypti neuronal tissues reveals 
            differential importance of chemosensory systems for 
            life-history-specific foraging strategies.
  JOURNAL   BMC Neurosci. 2019 Jun 17;20(1):27. doi: 10.1186/s12868-019-0511-y.
  PUBMED    31208328
REFERENCE   2  (bases 1 to 10465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Neurosci."; date: "2019-06-17"; volume: "20(1):27. doi"; pages: " 
            10.1186/s12868-019-0511-y"
COMMENT     Sequence Label: AE-Polyubiquitin-GCaMP6s
FEATURES             Location/Qualifiers
     source          1..10465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..70
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             join(1665..1673,1674..1691,1692..1694,1695..1727,1728..1730,
                     1731..1754,1755..1781,1782..1838,1839..1844,1845..2114,
                     2115..2132,2133..2567,2568..2573,2574..3017)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      1761..1770
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2051..2072
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2178..2199)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2439..2458)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             join(4252..4254,4255..4257,4258..4932)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(4435..4455)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     4653..4672
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4847..4870
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    5046..5167
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5083..5102)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5137..5156
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5835..5869
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6050..6066)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6050..6066)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6063..6085)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6074..6090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6098..6104,6105..6122,6123..6128))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6143..6164
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6281..6298)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6452..7040)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6532..6551)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7211..8002,8003..8071))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7834..7853
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8072..8176)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8244..8262
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8300..8322)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8422..8441
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8635..8657
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8649..8666
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8650..8666
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   9370..9432
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ctcgaagccg cggtgcgggt gccagggcgt gcccttgggc tccccgggcg cgtactccac
       61 ctcacccatc tggtccatca tgatgaacgg gtcgaggtgg cggtagttga tcccggcgaa
      121 cgcgcggcgc accgggaagc cctcgccctc gaaaccgctg ggcgcggtgg tcacggtgag
      181 cacgggacgt gcgacggcgt cggcgggtgc ggatacgcgg ggcagcgtca gcgggttctc
      241 gacggtcacg gcgggcatgt cgacgcggcc gctatcttta catgtagctt gtgcattgaa
      301 tccaattata atttgccttg gcaccagctg agccagacaa gaaagaaagc ttcccagaag
      361 tatatcgatt tagaagggtt gacgtcactt tgctgactgc actaatacag caaatgatac
      421 aattagaatg attcaagtga aattcccaaa ttactgcttt gtctctggat ttggttatca
      481 gattacattc gaagctaaga atagctaccg aaattgtcga tcaaatcagg aaatcctttc
      541 tctatcgaaa aaggcattcg cacatctttc tctgtatgcc atatacacga atggtaggta
      601 cattgacgtc tttgccagaa gttgaactgc atcgttcaag gtacagaatg aacgactaac
      661 agacacaagc acgttttgct gtccattcag acacagggat ggtacccata gtcgatcgat
      721 ttagagccat ccaaccgaac agaggtatat gtatgaatgg attgcagaaa ttttctagaa
      781 gtacaaccac cactacggca gtgtctataa aacgcccctg caaaggcaaa accagctcaa
      841 tcgaatacgt ttcctagtgg agtgaacatt acgcggtcca agtaagcagt gccagtgcaa
      901 gtgaagtgaa gtctctagtg aaaaagagtg atccaattag ccagaggaga aaatttcaga
      961 gtgaacaaag ctttgttcaa aggacaatta ctattaaatt tgtgaaagtg catttcggtg
     1021 aagggaatct tctagtgaag gtaggtaaat taaatgatga aattatagct atgagcgaaa
     1081 actagtttgg tgaatgattc ctttgtcttt gaatgagcaa actattttcc aagatggcga
     1141 ctattgagct ttgagtgatt agtgaaaatt tgcaacgcag tttcatcatc attgataaaa
     1201 cccaattgtg attcacggcg ataatcatat ttcgttgaat catcgctgct aattgaatta
     1261 aatttctaga gcaagcgcga attcgccata tttctaaaat taaatattgt ggtgataatt
     1321 acccattaag gtaatattaa cacatatcga gaaaaacctt gaggaaatcg tgaaaacttg
     1381 aagatacgca atttccaaac tacgtagttc aaagtcgaaa acaagttaat ttttcactaa
     1441 aaagtagggc gttgttgtga cgtcatcacc ttcaagtgta tatttttcac ttggcctgcg
     1501 actgcaaacg cagacaaagc aaaacaagtt taaaacctgt cgtgtcgtgc tcgaagccaa
     1561 aggcaatgaa tcaatatcaa atgagagttt gcatttcaca accaattact gaagcgtttc
     1621 ctcgtttctt tttctgctca acagagattt caacgaatct cgagatgggt tctcatcatc
     1681 atcatcatca tggtatggct agcatgactg gtggacagca aatgggtcgg gatctgtacg
     1741 acgatgacga taaggatctc gccaccatgg tcgactcatc acgtcgtaag tggaataaga
     1801 caggtcacgc agtcagagct ataggtcggc tgagctcact cgagaacgtc tatatcaagg
     1861 ccgacaagca gaagaacggc atcaaggcga acttccacat ccgccacaac atcgaggacg
     1921 gcggcgtgca gctcgcctac cactaccagc agaacacccc catcggcgac ggccccgtgc
     1981 tgctgcccga caaccactac ctgagcgtgc agtccaaact ttcgaaagac cccaacgaga
     2041 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     2101 acgagctgta caagggcggt accggaggga gcatggtgag caagggcgag gagctgttca
     2161 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     2221 tgtccggcga gggtgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     2281 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     2341 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     2401 ccgaaggcta catccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     2461 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     2521 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacctg ccggaccaac
     2581 tgactgaaga gcagatcgca gaatttaaag aggctttctc cctatttgac aaggacgggg
     2641 atgggacaat aacaaccaag gagctgggga cggtgatgcg gtctctgggg cagaacccca
     2701 cagaagcaga gctgcaggac atgatcaatg aagtagatgc cgacggtgac ggcacaatcg
     2761 acttccctga gttcctgaca atgatggcaa gaaaaatgaa atacagggac acggaagaag
     2821 aaattagaga agcgttcggt gtgtttgata aggatggcaa tggctacatc agtgcagcag
     2881 agcttcgcca cgtgatgaca aaccttggag agaagttaac agatgaagag gttgatgaaa
     2941 tgatcaggga agcagacatc gatggggatg gtcaggtaaa ctacgaagag tttgtacaaa
     3001 tgatgacagc gaagtgatta attaactaga atgaatcgtt tttaaaataa caaatcaatt
     3061 gttttataat attcgtacga ttctttgatt atgtaataaa atgtgatcat taggaagatt
     3121 acgaaaaata taaaaaatat gagttctgtg tgtataacaa atgctgtaaa cgccacaatt
     3181 gtgtttgttg caaataaacc catgattatt tgattaaaat tgttgttttc tttgttcata
     3241 gacaatagtg tgttttgcct aaacgtgtac tgcataaact ccatgcgagt gtatagcgag
     3301 ctagtggcta acgcttgccc caccaaagta gattcgtcaa aatcctcaat ttcatcaccc
     3361 tcctccaagt ttaacatttg gccgtcggaa ttaacttcta aagatgccac ataatctaat
     3421 aaatgaaata gagattcaaa cgtggcgtca tcgtccgttt cgaccatttc cgaaaagaac
     3481 tcgggcataa actctatgat ttctctggac gtggtgttgt cgaaactctc aaagtacgca
     3541 gtcaggaacg tgcgcgacat gtcgtcggga aactcgcgcg gaaacatgtt gttgtaaccg
     3601 aacgggtccc atagcgccaa aaccaaatct gccagcgtca atagaatgag cacgatgccg
     3661 acaatggagc tggcttggat agcgattcga gttaacggcc ggggcgcgcc catactcggt
     3721 ggcctcccca ccaccaactt ttttgcactg caaaaaaaca cgcttttgca cgcgggccca
     3781 tacatagtac aaactctacg tttcgtagac tattttacat aaatagtcta caccgttgta
     3841 tacgctccaa atacactacc acacattgaa cctttttgca gtgcaaaaaa gtacgtgtcg
     3901 gcagtcacgt aggccggcct tatcgggtcg cgtcctgtca cgtacgaatc acattatcgg
     3961 accggacgag tgttgtctta tcgtgacagg acgccagctt cctgtgttgc taaccgcagc
     4021 cggacgcaac tccttatcgg aacaggacgc gcctccatat cagccgcgcg ttatctcatg
     4081 cgcgtgaccg gacacgaggc gcccgtcccg cttatcgcgc ctataaatac agcccgcaac
     4141 gatctggtaa acacagttga acagcatctg ttacagcgac acaacatgag ccggtccaac
     4201 aacgccaacg cgcccacgcc atccaaccgc cgccgcaacc tgtctctggt gatggtgcgc
     4261 tcctccaaga acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggcaccgtg
     4321 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggccacaac
     4381 accgtgaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
     4441 ccccagttcc agtacggctc caaggtgtac gtgaagcacc ccgccgacat ccccgactac
     4501 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     4561 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gctgcttcat ctacaaggtg
     4621 aagttcatcg gcgtgaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     4681 tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac
     4741 aaggccctga agctgaagga cggcggccac tacctggtgg agttcaagtc catctacatg
     4801 gccaagaagc ccgtgcagct gcccggctac tactacgtgg actccaagct ggacatcacc
     4861 tcccacaacg aggactacac catcgtggag cagtacgagc gcaccgaggg ccgccaccac
     4921 ctgttcctgt aggactctag atcataatca gccataccac atttgtagag gttttacttg
     4981 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     5041 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5101 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5161 tatcttaaag cttctaggcc ggccgatctc ggatctgaca atgttcagtg cagagactcg
     5221 gctacgcctc gtggactttg aagttgacca acaatgttta ttcttacctc taatagtcct
     5281 ctgtggcaag gtcaagattc tgttagaagc caatgaagaa cctggttgtt caataacatt
     5341 ttgttcgtct aatatttcac taccgcttga cgttggctgc acttcatgta cctcatctat
     5401 aaacgcttct tctgtatcgc tctggacgtc atcttcactt acgtgatctg atatttcact
     5461 gtcagaatcc tcaccaacaa gctcgtcatc gctttgcaga agagcagaga ggatatgctc
     5521 atcgtctaaa gaactaccca ttttattata tattagtcac gatatctata acaagaaaat
     5581 atatatataa taagttatca cgtaagtaga acatgaaata acaatataat tatcgtatga
     5641 gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca cgcggtcgtt
     5701 atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg agctccaagc
     5761 ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa agagagagca
     5821 atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt taaaaaagat
     5881 ttgcgcttta ctcgacctaa actttaaaca cgtcatagaa tcttcgtttg acaaaaacca
     5941 cattgtggcc aagctgtgtg acgcgacgcg cgctaaagaa tggcaaacca agtcgcgcga
     6001 gcgtcgactc tagaggatcc ccgggtaccg agctcgaatt cgtaatcatg tcatagctgt
     6061 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6121 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6181 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6241 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6301 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6361 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6421 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6481 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6541 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6601 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6661 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6721 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6781 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6841 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6901 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6961 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7021 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7081 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7141 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7201 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7261 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7321 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7381 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7441 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7501 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7561 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7621 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7681 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7741 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7801 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7861 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7921 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7981 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8041 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8101 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8161 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     8221 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     8281 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     8341 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     8401 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     8461 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
     8521 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     8581 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     8641 cacgacgttg taaaacgacg gccagtgcca agctttgttt aaaaatataa caaaattgtg
     8701 atcccacaaa atgaagtggg gcaaaatcaa ataattaata gtgtccgtaa acttgttggt
     8761 cttcaacttt ttgaggaaca cgttggacgg caaatccgtg actataacac aagttgattt
     8821 aataatttta gccaacacgt cgggctgcgt gttttttgcc gacgcgtctg tgtacacgtt
     8881 gattaactgg tcgattaaac tgttgaaata atttaatttt tggttcttct ttaaatctgt
     8941 gatgaaattt tttaaaataa ctttaaattc ttcattggta aaaaatgcca cgttttgcaa
     9001 cttgtgaggg tctaatatga ggtcaaactc agtaggagtt ttatccaaaa aagaaaacat
     9061 gattacgtct gtacacgaac gcgtattaac gcagagtgca aagtataaga gggttaaaaa
     9121 atatatttta cgcaccatat acgcatcggg ttgatatcgt taatatggat caatttgaac
     9181 agttgattaa cgtgtctctg ctcaagtctt tgatcaaaac gcaaatcgac gaaaatgtgt
     9241 cggacaatat caagtcgatg agcgaaaaac taaaaaggct agaatacgac aatctcacag
     9301 acagcgttga gatatacggt attcacgaca gcaggctgaa taataaaaaa attagaaact
     9361 attatttaac cctagaaaga taatcatatt gtgacgtacg ttaaagataa tcatgcgtaa
     9421 aattgacgca tgtgttttat cggtctgtat atcgaggttt atttattaat ttgaatagat
     9481 attaagtttt attatattta cacttacata ctaataataa attcaacaaa caatttattt
     9541 atgtttattt atttattaaa aaaaaacaaa aactcaaaat ttcttctata aagtaacaaa
     9601 acttttaaac attctctctt ttacaaaaat aaacttattt tgtactttaa aaacagtcat
     9661 gttgtattat aaaataagta attagcttaa cttatacata atagaaacaa attatactta
     9721 ttagtcagtc agaaacaact ttggcacata tcaatattat gctctcgaca aataactttt
     9781 ttgcattttt tgcacgatgc atttgccttt cgccttattt tagaggggca gtaagtacag
     9841 taagtacgtt ttttcattac tggctcttca gtactgtcat ctgatgtacc aggcacttca
     9901 tttggcaaaa tattagagat attatcgcgc aaatatctct tcaaagtagg agcttctaaa
     9961 cgcttacgca taaacgatga cgtcaggctc atgtaaaggt ttctcataaa ttttttgcga
    10021 ctttgaacct tttctccctt gctactgaca ttatggctgt atataataaa agaatttatg
    10081 caggcaatgt ttatcattcc gtacaataat gccataggcc acctattcgt cttcctactg
    10141 caggtcatca cagaacacat ttggtctagc gtgtccactc cgcctttagt ttgattataa
    10201 tacataacca tttgcggttt accggtactt tcgttgatag aagcatcctc atcacaagat
    10261 gataataagt ataccatctt agctggcttc ggtttatatg agacgagagt aaggggtccg
    10321 tcaaaacaaa acatcgatgt tcccactggc ctggagcgac tgtttttcag tacttccggt
    10381 atctcgcgtt tgtttgatcg cacggttccc acaatggtta attcgagctc gcccggggtc
    10441 ctaggtcgac gatgtaggtc acggt
//
