LOCUS       sequence-209392-       15313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ABAleon2.15 in plant expression vector (Hygromycin resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15313)
  AUTHORS   Waadt R, Hitomi K, Nishimura N, Hitomi C, Adams SR, Getzoff ED, 
            Schroeder JI
  TITLE     FRET-based reporters for the direct visualization of abscisic acid 
            concentration changes and distribution in Arabidopsis.
  JOURNAL   Elife. 2014 Apr 15;3:e01739. doi: 10.7554/eLife.01739.
  PUBMED    24737861
REFERENCE   2  (bases 1 to 15313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-04-15"; volume: "3:e01739. doi"; pages: " 
            10.7554/eLife.01739"
COMMENT     Sequence Label: hygII-UT-ABAleon2.15
FEATURES             Location/Qualifiers
     source          1..15313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2320..3054
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     misc_feature    3478..3502
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3967..4617
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(4805..4822)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5056..5075)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            5298..5407
                     /note="incP origin of transfer"
     CDS             5440..5811
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      5938..6649
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  7272..8039
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             8231..9025
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             9324..10472
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    12068..12092
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(12339..12360)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12551..12570
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12695..12717
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12709..12726
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12710..12726
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(12739..12939)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     CDS             complement(12978..14003)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(14057..14236)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacat cagcgacaac gtctatatca ccgccgacaa gcagaagaac
      481 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggg
      721 cccatgcctt cggagttaac accagaagaa cgatcggaac taaaaaactc aatcgccgag
      781 ttccacacat accaactcga tccaggaagc tgttcatcac tccacgcgca acgaatccac
      841 gcgcctccgg aactcgtctg gtcaatcgta cgacgattcg acaaaccaca aacatacaaa
      901 cacttcatca aatcctgctc cgtcgaacaa aacttcgaga tgcgcgtcgg atgcacgcgc
      961 gacgtgatcg tcatcagtgg attaccggcg aacacatcaa cggaaagact cgatatactc
     1021 gacgacgaac ggagagttac cggattcagt atcatcggag gcgaagctag gctgacgaat
     1081 tacaaatccg ttacgacggt gcatcggttc gagaaagaga atcggatctg gacggtggtt
     1141 ttggaatctt acgtcgttga tatgccggaa ggtaactcgg aggatgatac tcgtatgttt
     1201 gctgatacgg ttgtgaagct taatttgcag aaactcgcga cggttgctga agctatggct
     1261 cgtaactccg gtgacggaag tggttctcag gtgacggcta gcggtggcag cggtggcact
     1321 agtggtggag gcggatccgg tggaggaggc tctggtggcg gaggtagcgg aggcggtgga
     1381 tccagtgtgc ctttgtatgg ttttacttcg atttgtggaa gaagacctga gatggaagat
     1441 gctgtttcga ctataccaag attccttcaa tcttcctctg gttcgatgtt agatggtcgg
     1501 tttgatcctc aatccgccgc tcatttcttc ggtgtttacg acggccatgg cggttctcag
     1561 gtagcgaact attgtagaga gaggatgcat ttggctttgg cggaggagat agctaaggag
     1621 aaaccgatgc tctgcgatgg tgatacgtgg ctggagaagt ggaagaaagc tcttttcaac
     1681 tcgttcctga gagttgactc ggagattgag tcagttgcgc cggagacggt tgggtcaacg
     1741 tcggtggttg ccgttgtttt cccgtctcac atcttcgtcg ctaactgcgg tgactcaaga
     1801 gccgttcttt gccgcggcaa aactgcactt ccattatccg ttgaccataa accggataga
     1861 gaagatgaag ctgcgaggat tgaagccgca ggagggaaag tgattcagtg gaatggtgct
     1921 cgtgttttcg gtgttctcgc catgtcgaga tccattggcg atagatactt gaaaccatcc
     1981 atcattcctg atccggaagt gacggctgtg aagagagtaa aagaagatga ttgtctgatt
     2041 ttggcgagtg acggggtttg ggatgtaatg acggatgaag aagcgtgtga gatggcaagg
     2101 aagcggattc tcttgtggca caagaaaaac gcggtggctg gggatgcatc gttgctcgcg
     2161 gatgagcgga gaaaggaagg gaaagatcct gcggcgatgt ccgcggctga gtatttgtca
     2221 aagctggcga tacagagagg aagcaaactc aacataagtg tggtggtggt tgatttgaag
     2281 cctcggagga aactcaagag caaacccttg aaccccggga tggacggcgg cgtgcagctc
     2341 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     2401 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     2461 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     2521 ggtggcagcg gtggcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     2581 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     2641 ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc
     2701 gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac
     2761 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     2821 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     2881 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     2941 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc
     3001 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgagtaggag
     3061 ctcatatgaa gatgaagatg aaatatttgg tgtgtcaaat aaatagcttg tgtgcttaag
     3121 tttgtgtttt tttcttggct tgttgtgtta tgaatttgtg gctttttcta atattaaatg
     3181 aatgtaagat ctcattataa tgaataaaca aatgtttcta taatccattg tgaatgtttt
     3241 gttggatctc ttctgcagca tataactact gtatgtgcta tggtatggac tatggaatat
     3301 gattaaagat aaggaattca gatcggctga gtggctcctt caacgttgcg gttctgtcag
     3361 ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt gactccctta
     3421 attctccgct catgatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga
     3481 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
     3541 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
     3601 ttccccagat ctggcgccgg ccagcgagac gagcaagatt ggccgccgcc cgaaacgatc
     3661 cgacagcgcg cccagcacag gtgcgcaggc aaattgcacc aacgcataca gcgccagcag
     3721 aatgccatag tgggcggtga cgtcgttcga gtgaaccaga tcgcgcagga ggcccggcag
     3781 caccggcata atcaggccga tgccgacagc gtcgagcgcg acagtgctca gaattacgat
     3841 caggggtatg ttgggtttca cgtctggcct ccggaccagc ctccgctggt ccgattgaac
     3901 gcgcggattc tttatcactg ataagttggt ggacatatta tgtttatcag tgataaagtg
     3961 tcaagcatga caaagttgca gccgaataca gtgatccgtg ccgccctgga cctgttgaac
     4021 gaggtcggcg tagacggtct gacgacacgc aaactggcgg aacggttggg ggttcagcag
     4081 ccggcgcttt actggcactt caggaacaag cgggcgctgc tcgacgcact ggccgaagcc
     4141 atgctggcgg agaatcatac gcattcggtg ccgagagccg acgacgactg gcgctcattt
     4201 ctgatcggga atgcccgcag cttcaggcag gcgctgctcg cctaccgcga tggcgcgcgc
     4261 atccatgccg gcacgcgacc gggcgcaccg cagatggaaa cggccgacgc gcagcttcgc
     4321 ttcctctgcg aggcgggttt ttcggccggg gacgccgtca atgcgctgat gacaatcagc
     4381 tacttcactg ttggggccgt gcttgaggag caggccggcg acagcgatgc cggcgagcgc
     4441 ggcggcaccg ttgaacaggc tccgctctcg ccgctgttgc gggccgcgat agacgccttc
     4501 gacgaagccg gtccggacgc agcgttcgag cagggactcg cggtgattgt cgatggattg
     4561 gcgaaaagga ggctcgttgt caggaacgtt gaaggaccga gaaagggtga cgattgatca
     4621 ggaccgctgc cggagcgcaa cccactcact acagcagagc catgtagaca acatcccctc
     4681 cccctttcca ccgcgtcaga cgcccgtagc agcccgctac gggctttttc atgccctgcc
     4741 ctagcgtcca agcctcacgg ccgcgctcgg cctctctggc ggccttctgg cgctcttccg
     4801 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4861 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4921 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4981 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5041 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5101 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5161 cgcttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca
     5221 tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt
     5281 atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc
     5341 ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc
     5401 tggccggcta ccgccggcgt aacagatgag ggcaagcgga tggctgatga aaccaagcca
     5461 accaggaagg gcagcccacc tatcaaggtg tactgccttc cagacgaacg aagagcgatt
     5521 gaggaaaagg cggcggcggc cggcatgagc ctgtcggcct acctgctggc cgtcggccag
     5581 ggctacaaaa tcacgggcgt cgtggactat gagcacgtcc gcgagctggc ccgcatcaat
     5641 ggcgacctgg gccgcctggg cggcctgctg aaactctggc tcaccgacga cccgcgcacg
     5701 gcgcggttcg gtgatgccac gatcctcgcc ctgctggcga agatcgaaga gaagcaggac
     5761 gagcttggca aggtcatgat gggcgtggtc cgcccgaggg cagagccatg acttttttag
     5821 ccgctaaaac ggccgggggg tgcgcgtgat tgccaagcac gtccccatgc gctccatcaa
     5881 gaagagcgac ttcgcggagc tggtgaagta catcaccgac gagcaaggca agaccgagcg
     5941 cctttccgac gctcaccggg ctggttgccc tcgccgctgg gctggcggcc gtctatggcc
     6001 ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag acaccgcggc cgccggcgtt
     6061 gtggatacct cgcggaaaac ttggccctca ctgacagatg aggggcggac gttgacactt
     6121 gaggggccga ctcacccggc gcggcgttga cagatgaggg gcaggctcga tttcggccgg
     6181 cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc
     6241 cacagatgat gtggacaagc ctggggataa gtgccctgcg gtattgacac ttgaggggcg
     6301 cgactactga cagatgaggg gcgcgatcct tgacacttga ggggcagagt gctgacagat
     6361 gaggggcgca cctattgaca tttgaggggc tgtccacagg cagaaaatcc agcatttgca
     6421 agggtttccg cccgtttttc ggccaccgct aacctgtctt ttaacctgct tttaaaccaa
     6481 tatttataaa ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga
     6541 aggggggtgc ccccccttct cgaaccctcc cggcccgcta acgcgggcct cccatccccc
     6601 caggggctgc gcccctcggc cgcgaacggc ctcaccccaa aaatggcagc gctggcagtc
     6661 cttgccattg ccgggatcgg ggcagtaacg ggatgggcga tcagcccgag cgcgacgccc
     6721 ggaagcattg acgtgccgca ggtgctggca tcgacattca gcgaccaggt gccgggcagt
     6781 gagggcggcg gcctgggtgg cggcctgccc ttcacttcgg ccgtcggggc attcacggac
     6841 ttcatggcgg ggccggcaat ttttaccttg ggcattcttg gcatagtggt cgcgggtgcc
     6901 gtgctcgtgt tcgggggtgc gataaaccca gcgaaccatt tgaggtgata ggtaagatta
     6961 taccgaggta tgaaaacgag aattggacct ttacagaatt actctatgaa gcgccatatt
     7021 taaaaagcta ccaagacgaa gaggatgaag aggatgagga ggcagattgc cttgaatata
     7081 ttgacaatac tgataagata atatatcttt tatatagaag atatcgccgt atgtaaggat
     7141 ttcagggggc aaggcatagg cagcgcgctt atcaatatat ctatagaatg ggcaaagcat
     7201 aaaaacttgc atggactaat gcttgaaacc caggacaata accttatagc ttgtaaattc
     7261 tatcataatt gggtaatgac tccaacttat tgatagtgtt ttatgttcag ataatgcccg
     7321 atgactttgt catgcagctc caccgatttt gagaacgaca gcgacttccg tcccagccgt
     7381 gccaggtgct gcctcagatt caggttatgc cgctcaattc gctgcgtata tcgcttgctg
     7441 attacgtgca gctttccctt caggcgggat tcatacagcg gccagccatc cgtcatccat
     7501 atcaccacgt caaagggtga cagcaggctc ataagacgcc ccagcgtcgc catagtgcgt
     7561 tcaccgaata cgtgcgcaac aaccgtcttc cggagactgt catacgcgta aaacagccag
     7621 cgctggcgcg atttagcccc gacatagccc cactgttcgt ccatttccgc gcagacgatg
     7681 acgtcactgc ccggctgtat gcgcgaggtt accgactgcg gcctgagttt tttaagtgac
     7741 gtaaaatcgt gttgaggcca acgcccataa tgcgggctgt tgcccggcat ccaacgccat
     7801 tcatggccat atcaatgatt ttctggtgcg taccgggttg agaagcggtg taagtgaact
     7861 gcagttgcca tgttttacgg cagtgagagc agagatagcg ctgatgtccg gcggtgcttt
     7921 tgccgttacg caccaccccg tcagtagctg aacaggaggg acagctgata gaaacagaag
     7981 ccactggagc acctcaaaaa caccatcata cactaaatca gtaagttggc agcatcaccc
     8041 ataattgtgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac tttgaaaaca
     8101 actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac agtgaattgg
     8161 agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga aaagaggaag
     8221 gaaataataa atggctaaaa tgagaatatc accggaattg aaaaaactga tcgaaaaata
     8281 ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc tggtgggaga
     8341 aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca cctatgatgt
     8401 ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc caaaggtcct
     8461 gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg atggcgtcct
     8521 ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc tgtatgcgga
     8581 gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga atagcttaga
     8641 cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg tggattgcga
     8701 aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt ttttaaagac
     8761 ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca gcaacatctt
     8821 tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca gggcggacaa
     8881 gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg aagaacagta
     8941 tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatta
     9001 tattttactg gatgaattgt tttagtacct agatgtggcg caacgatgcc ggcgacaagc
     9061 aggagcgcac cgacttcttc cgcatcaagt gttttggctc tcaggccgag gcccacggca
     9121 agtatttggg caaggggtcg ctggtattcg tgcagggcaa gattcggaat accaagtacg
     9181 agaaggacgg ccagacggtc tacgggaccg acttcattgc cgataaggtg gattatctgg
     9241 acaccaaggc accaggcggg tcaaatcagg aataagggca cattgccccg gcgtgagtcg
     9301 gggcaatccc gcaaggaggg tgaatgaatc ggacgtttga ccggaaggca tacaggcaag
     9361 aactgatcga cgcggggttt tccgccgagg atgccgaaac catcgcaagc cgcaccgtca
     9421 tgcgtgcgcc ccgcgaaacc ttccagtccg tcggctcgat ggtccagcaa gctacggcca
     9481 agatcgagcg cgacagcgtg caactggctc cccctgccct gcccgcgcca tcggccgccg
     9541 tggagcgttc gcgtcgtctc gaacaggagg cggcaggttt ggcgaagtcg atgaccatcg
     9601 acacgcgagg aactatgacg accaagaagc gaaaaaccgc cggcgaggac ctggcaaaac
     9661 aggtcagcga ggccaagcag gccgcgttgc tgaaacacac gaagcagcag atcaaggaaa
     9721 tgcagctttc cttgttcgat attgcgccgt ggccggacac gatgcgagcg atgccaaacg
     9781 acacggcccg ctctgccctg ttcaccacgc gcaacaagaa aatcccgcgc gaggcgctgc
     9841 aaaacaaggt cattttccac gtcaacaagg acgtgaagat cacctacacc ggcgtcgagc
     9901 tgcgggccga cgatgacgaa ctggtgtggc agcaggtgtt ggagtacgcg aagcgcaccc
     9961 ctatcggcga gccgatcacc ttcacgttct acgagctttg ccaggacctg ggctggtcga
    10021 tcaatggccg gtattacacg aaggccgagg aatgcctgtc gcgcctacag gcgacggcga
    10081 tgggcttcac gtccgaccgc gttgggcacc tggaatcggt gtcgctgctg caccgcttcc
    10141 gcgtcctgga ccgtggcaag aaaacgtccc gttgccaggt cctgatcgac gaggaaatcg
    10201 tcgtgctgtt tgctggcgac cactacacga aattcatatg ggagaagtac cgcaagctgt
    10261 cgccgacggc ccgacggatg ttcgactatt tcagctcgca ccgggagccg tacccgctca
    10321 agctggaaac cttccgcctc atgtgcggat cggattccac ccgcgtgaag aagtggcgcg
    10381 agcaggtcgg cgaagcctgc gaagagttgc gaggcagcgg cctggtggaa cacgcctggg
    10441 tcaatgatga cctggtgcat tgcaaacgct agggccttgt ggggtcagtt ccggctgggg
    10501 gttcagcagc cagcgcttta ctggcatttc aggaacaagc gggcactgct cgacgcactt
    10561 gcttcgctca gtatcgctcg ggacgcacgg cgcgctctac gaactgccga taaacagagg
    10621 attaaaattg acaattgtga ttaaggctca gattcgacgg cttggagcgg ccgacgtgca
    10681 ggatttccgc gagatccgat tgtcggccct gaagaaagct ccagagatgt tcgggtccgt
    10741 ttacgagcac gaggagaaaa agcccatgga ggcgttcgct gaacggttgc gagatgccgt
    10801 ggcattcggc gcctacatcg acggcgagat cattgggctg tcggtcttca aacaggagga
    10861 cggccccaag gacgctcaca aggcgcatct gtccggcgtt ttcgtggagc ccgaacagcg
    10921 aggccgaggg gtcgccggta tgctgctgcg ggcgttgccg gcgggtttat tgctcgtgat
    10981 gatcgtccga cagattccaa cgggaatctg gtggatgcgc atcttcatcc tcggcgcact
    11041 taatatttcg ctattctgga gcttgttgtt tatttcggtc taccgcctgc cgggcggggt
    11101 cgcggcgacg gtaggcgctg tgcagccgct gatggtcgtg ttcatctctg ccgctctgct
    11161 aggtagcccg atacgattga tggcggtcct gggggctatt tgcggaactg cgggcgtggc
    11221 gctgttggtg ttgacaccaa acgcagcgct agatcctgtc ggcgtcgcag cgggcctggc
    11281 gggggcggtt tccatggcgt tcggaaccgt gctgacccgc aagtggcaac ctcccgtgcc
    11341 tctgctcacc tttaccgcct ggcaactggc ggccggagga cttctgctcg ttccagtagc
    11401 tttagtgttt gatccgccaa tcccgatgcc tacaggaacc aatgttctcg gcctggcgtg
    11461 gctcggcctg atcggagcgg gtttaaccta cttcctttgg ttccggggga tctcgcgact
    11521 cgaacctaca gttgtttcct tactgggctt tctcagcccc agatctgggg tcgatcagcc
    11581 ggggatgcat caggccgaca gtcggaactt cgggtccccg acctgtacca ttcggtgagc
    11641 aatggatagg ggagttgata tcgtcaacgt tcacttctaa agaaatagcg ccactcagct
    11701 tcctcagcgg ctttatccag cgatttccta ttatgtcggc atagttctca agatcgacag
    11761 cctgtcacgg ttaagcgaga aatgaataag aaggctgata attcggatct ctgcgaggga
    11821 gatgatattt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc
    11881 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg
    11941 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg
    12001 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
    12061 tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca
    12121 cattgcggac gtttttaatg tactggggtg gtttttcttt tcaccagtga gacgggcaac
    12181 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt
    12241 tgccccagca ggcgaaaatc ctgtttgatg gtggttccga aatcggcaaa atcccttata
    12301 aatcaaaaga atagcccgag atagggttga gtgttgttcc agtttggaac aagagtccac
    12361 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
    12421 cactacgtga accatcaccc aaatcaagtt ttttggggtc gaggtgccgt aaagcactaa
    12481 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
    12541 cgagaaagga agggaagaaa gcgaaaggag cgggcgccat tcaggctgcg caactgttgg
    12601 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
    12661 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
    12721 gccagtgaat taattcccat cttgaaagaa atatagttta aatatttatt gataaaataa
    12781 caagtcaggt attatagtcc aagcaaaaac ataaatttat tgatgcaagt ttaaattcag
    12841 aaatatttca ataactgatt atatcagctg gtacattgcc gtagatgaaa gactgagtgc
    12901 gatattatgt gtaatacata aattgatgat atagctagct tagctcatcg ggggatcgat
    12961 cccggtcggc atctactcta ttcctttgcc ctcggacgag tgctggggcg tcggtttcca
    13021 ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt
    13081 tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc
    13141 caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg
    13201 cggagcatat acgcccggag ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag
    13261 tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc
    13321 tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca
    13381 ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg
    13441 cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg
    13501 tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca
    13561 cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg
    13621 ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga ccggctgcag aacagcgggc
    13681 agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag
    13741 gtcaggctct cgctgaatgc cccaatgtca agcacttccg gaatcgggag cgcggccgat
    13801 gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc
    13861 aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag
    13921 agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc
    13981 gcggtgagtt caggcttttt catatcttat tgccccccta gagtcgagat ctggattgag
    14041 agtgaatatg agactctaat tggataccga ggggaattta tggaacgtca gtggagcatt
    14101 tttgacaaga aatatttgct agctgatagt gaccttaggc gacttttgaa cgcgcaataa
    14161 tggtttctga cgtatgtgct tagctcatta aactccagaa acccgcggct gagtggctcc
    14221 ttcaacgttg cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg
    14281 gggtcataac gtgactccct taattctccg ctcatgatct tgatcccctg cgccatcaga
    14341 tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg
    14401 gcgccccagc tggcaattcc ggttcgcttg ctgtccataa aaccgcccag tctagctatc
    14461 gccatgtaag cccactgcaa gctacctgct ttctctttgc gcttgcgttt tcccttgtcc
    14521 agatagccca gtagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct
    14581 acgtgttccg cttcctttag cagcccttgc gccctgagtg cttgcggcag cgtgaagctt
    14641 cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg agtcgtgttt
    14701 atcaactcaa agcacaaata cttttcctca acctaaaaat aaggcaatta gccaaaaaca
    14761 actttgcgtg taaacaacgc tcaatacacg tgtcatttta ttattagcta ttgcttcacc
    14821 gccttagctt tctcgtgacc tagtcgtcct cgtcttttct tcttcttctt ctataaaaca
    14881 atacccaaag acctcttctt cttcacaatt cagatttcaa tttctcaaaa tcttaaaaac
    14941 tttctctcaa ttctctctac cgtgatcaag gtaaatttct gtgttcctta ttctctcaaa
    15001 atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat gttctttggt ttagattctg
    15061 ttaatcttag atcgaagacg attttctggg tttgatcgtt agatatcatc ttaattctcg
    15121 attagggttt catagatatc atccgatttg ttcaaataat ttgagttttg tcgaataatt
    15181 actcttcgat ttgtgatttc tatctagatc tggtgttagt ttctagtttg tgcgatcgaa
    15241 tttgtcgatt aatctgagtt tttctgatta acagactaga gttaaccggg ctcaggcctg
    15301 gcgcgccact aga
//
