LOCUS       sequence-209394-       15280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ABAleon2.1 in plant expression vector (Hygromycin resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15280)
  AUTHORS   Waadt R, Hitomi K, Nishimura N, Hitomi C, Adams SR, Getzoff ED, 
            Schroeder JI
  TITLE     FRET-based reporters for the direct visualization of abscisic acid 
            concentration changes and distribution in Arabidopsis.
  JOURNAL   Elife. 2014 Apr 15;3:e01739. doi: 10.7554/eLife.01739.
  PUBMED    24737861
REFERENCE   2  (bases 1 to 15280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-04-15"; volume: "3:e01739. doi"; pages: " 
            10.7554/eLife.01739"
COMMENT     Sequence Label: hygII-UT-ABAleon2.1
FEATURES             Location/Qualifiers
     source          1..15280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(641..643,644..646,647..1357)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2960..3694
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     misc_feature    4118..4142
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4607..5257
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(5445..5462)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5696..5715)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            5938..6047
                     /note="incP origin of transfer"
     CDS             6080..6451
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      6578..7289
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  7912..8679
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             8871..9665
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             9964..11112
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    12708..12732
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(12979..13000)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13191..13210
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13335..13357
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13349..13366
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13350..13366
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(13379..13579)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     CDS             complement(13618..14643)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(14697..14876)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg agtcgtgttt
       61 atcaactcaa agcacaaata cttttcctca acctaaaaat aaggcaatta gccaaaaaca
      121 actttgcgtg taaacaacgc tcaatacacg tgtcatttta ttattagcta ttgcttcacc
      181 gccttagctt tctcgtgacc tagtcgtcct cgtcttttct tcttcttctt ctataaaaca
      241 atacccaaag acctcttctt cttcacaatt cagatttcaa tttctcaaaa tcttaaaaac
      301 tttctctcaa ttctctctac cgtgatcaag gtaaatttct gtgttcctta ttctctcaaa
      361 atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat gttctttggt ttagattctg
      421 ttaatcttag atcgaagacg attttctggg tttgatcgtt agatatcatc ttaattctcg
      481 attagggttt catagatatc atccgatttg ttcaaataat ttgagttttg tcgaataatt
      541 actcttcgat ttgtgatttc tatctagatc tggtgttagt ttctagtttg tgcgatcgaa
      601 tttgtcgatt aatctgagtt tttctgatta acagactaga atggtgagca agggcgagga
      661 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      721 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      781 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgtcctg
      841 gggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
      901 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
      961 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     1021 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacat
     1081 cagcgacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa
     1141 gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac
     1201 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa
     1261 gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1321 cgccgggatc actctcggca tggacgagct gtacaagggg cccatgcctt cggagttaac
     1381 accagaagaa cgatcggaac taaaaaactc aatcgccgag ttccacacat accaactcga
     1441 tccaggaagc tgttcatcac tccacgcgca acgaatccac gcgcctccgg aactcgtctg
     1501 gtcaatcgta cgacgattcg acaaaccaca aacatacaaa cacttcatca aatcctgctc
     1561 cgtcgaacaa aacttcgaga tgcgcgtcgg atgcacgcgc gacgtgatcg tcatcagtgg
     1621 attaccggcg aacacatcaa cggaaagact cgatatactc gacgacgaac ggagagttac
     1681 cggattcagt atcatcggag gcgaacatag gctgacgaat tacaaatccg ttacgacggt
     1741 gcatcggttc gagaaagaga atcggatctg gacggtggtt ttggaatctt acgtcgttga
     1801 tatgccggaa ggtaactcgg aggatgatac tcgtatgttt gctgatacgg ttgtgaagct
     1861 taatttgcag aaactcgcga cggttgctga agctatggct cgtaactccg gtgacggaag
     1921 tggttctcag gtgacggcta gcggtggcag cggtggcact agtggtggag gcggatccgg
     1981 tggaggaggc tctggtggcg gaggtagcgg aggcggtgga tccagtgtgc ctttgtatgg
     2041 ttttacttcg atttgtggaa gaagacctga gatggaagat gctgtttcga ctataccaag
     2101 attccttcaa tcttcctctg gttcgatgtt agatggtcgg tttgatcctc aatccgccgc
     2161 tcatttcttc ggtgtttacg acggccatgg cggttctcag gtagcgaact attgtagaga
     2221 gaggatgcat ttggctttgg cggaggagat agctaaggag aaaccgatgc tctgcgatgg
     2281 tgatacgtgg ctggagaagt ggaagaaagc tcttttcaac tcgttcctga gagttgactc
     2341 ggagattgag tcagttgcgc cggagacggt tgggtcaacg tcggtggttg ccgttgtttt
     2401 cccgtctcac atcttcgtcg ctaactgcgg tgactcaaga gccgttcttt gccgcggcaa
     2461 aactgcactt ccattatccg ttgaccataa accggataga gaagatgaag ctgcgaggat
     2521 tgaagccgca ggagggaaag tgattcagtg gaatggtgct cgtgttttcg gtgttctcgc
     2581 catgtcgaga tccattggcg atagatactt gaaaccatcc atcattcctg atccggaagt
     2641 gacggctgtg aagagagtaa aagaagatga ttgtctgatt ttggcgagtg acggggtttg
     2701 ggatgtaatg acggatgaag aagcgtgtga gatggcaagg aagcggattc tcttgtggca
     2761 caagaaaaac gcggtggctg gggatgcatc gttgctcgcg gatgagcgga gaaaggaagg
     2821 gaaagatcct gcggcgatgt ccgcggctga gtatttgtca aagctggcga tacagagagg
     2881 aagcaaactc aacataagtg tggtggtggt tgatttgaag cctcggagga aactcaagag
     2941 caaacccttg aaccccggga tggacggcgg cgtgcagctc gccgaccact accagcagaa
     3001 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
     3061 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3121 cgccgccggg atcactctcg gcatggacga gctgtacaag ggtggcagcg gtggcatggt
     3181 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     3241 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     3301 gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     3361 gaccaccctg ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     3421 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     3481 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     3541 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     3601 ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat
     3661 caaggccaac ttcaagatcc gccacaacat cgagtaggag ctcatatgaa gatgaagatg
     3721 aaatatttgg tgtgtcaaat aaatagcttg tgtgcttaag tttgtgtttt tttcttggct
     3781 tgttgtgtta tgaatttgtg gctttttcta atattaaatg aatgtaagat ctcattataa
     3841 tgaataaaca aatgtttcta taatccattg tgaatgtttt gttggatctc ttctgcagca
     3901 tataactact gtatgtgcta tggtatggac tatggaatat gattaaagat aaggaattca
     3961 gatcggctga gtggctcctt caacgttgcg gttctgtcag ttccaaacgt aaaacggctt
     4021 gtcccgcgtc atcggcgggg gtcataacgt gactccctta attctccgct catgatcaga
     4081 ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa
     4141 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
     4201 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttccccagat ctggcgccgg
     4261 ccagcgagac gagcaagatt ggccgccgcc cgaaacgatc cgacagcgcg cccagcacag
     4321 gtgcgcaggc aaattgcacc aacgcataca gcgccagcag aatgccatag tgggcggtga
     4381 cgtcgttcga gtgaaccaga tcgcgcagga ggcccggcag caccggcata atcaggccga
     4441 tgccgacagc gtcgagcgcg acagtgctca gaattacgat caggggtatg ttgggtttca
     4501 cgtctggcct ccggaccagc ctccgctggt ccgattgaac gcgcggattc tttatcactg
     4561 ataagttggt ggacatatta tgtttatcag tgataaagtg tcaagcatga caaagttgca
     4621 gccgaataca gtgatccgtg ccgccctgga cctgttgaac gaggtcggcg tagacggtct
     4681 gacgacacgc aaactggcgg aacggttggg ggttcagcag ccggcgcttt actggcactt
     4741 caggaacaag cgggcgctgc tcgacgcact ggccgaagcc atgctggcgg agaatcatac
     4801 gcattcggtg ccgagagccg acgacgactg gcgctcattt ctgatcggga atgcccgcag
     4861 cttcaggcag gcgctgctcg cctaccgcga tggcgcgcgc atccatgccg gcacgcgacc
     4921 gggcgcaccg cagatggaaa cggccgacgc gcagcttcgc ttcctctgcg aggcgggttt
     4981 ttcggccggg gacgccgtca atgcgctgat gacaatcagc tacttcactg ttggggccgt
     5041 gcttgaggag caggccggcg acagcgatgc cggcgagcgc ggcggcaccg ttgaacaggc
     5101 tccgctctcg ccgctgttgc gggccgcgat agacgccttc gacgaagccg gtccggacgc
     5161 agcgttcgag cagggactcg cggtgattgt cgatggattg gcgaaaagga ggctcgttgt
     5221 caggaacgtt gaaggaccga gaaagggtga cgattgatca ggaccgctgc cggagcgcaa
     5281 cccactcact acagcagagc catgtagaca acatcccctc cccctttcca ccgcgtcaga
     5341 cgcccgtagc agcccgctac gggctttttc atgccctgcc ctagcgtcca agcctcacgg
     5401 ccgcgctcgg cctctctggc ggccttctgg cgctcttccg cttcctcgct cactgactcg
     5461 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     5521 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5581 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5641 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5701 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5761 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgcttttccg ctgcataacc
     5821 ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac
     5881 aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg
     5941 caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc
     6001 gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccggcta ccgccggcgt
     6061 aacagatgag ggcaagcgga tggctgatga aaccaagcca accaggaagg gcagcccacc
     6121 tatcaaggtg tactgccttc cagacgaacg aagagcgatt gaggaaaagg cggcggcggc
     6181 cggcatgagc ctgtcggcct acctgctggc cgtcggccag ggctacaaaa tcacgggcgt
     6241 cgtggactat gagcacgtcc gcgagctggc ccgcatcaat ggcgacctgg gccgcctggg
     6301 cggcctgctg aaactctggc tcaccgacga cccgcgcacg gcgcggttcg gtgatgccac
     6361 gatcctcgcc ctgctggcga agatcgaaga gaagcaggac gagcttggca aggtcatgat
     6421 gggcgtggtc cgcccgaggg cagagccatg acttttttag ccgctaaaac ggccgggggg
     6481 tgcgcgtgat tgccaagcac gtccccatgc gctccatcaa gaagagcgac ttcgcggagc
     6541 tggtgaagta catcaccgac gagcaaggca agaccgagcg cctttccgac gctcaccggg
     6601 ctggttgccc tcgccgctgg gctggcggcc gtctatggcc ctgcaaacgc gccagaaacg
     6661 ccgtcgaagc cgtgtgcgag acaccgcggc cgccggcgtt gtggatacct cgcggaaaac
     6721 ttggccctca ctgacagatg aggggcggac gttgacactt gaggggccga ctcacccggc
     6781 gcggcgttga cagatgaggg gcaggctcga tttcggccgg cgacgtggag ctggccagcc
     6841 tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc cacagatgat gtggacaagc
     6901 ctggggataa gtgccctgcg gtattgacac ttgaggggcg cgactactga cagatgaggg
     6961 gcgcgatcct tgacacttga ggggcagagt gctgacagat gaggggcgca cctattgaca
     7021 tttgaggggc tgtccacagg cagaaaatcc agcatttgca agggtttccg cccgtttttc
     7081 ggccaccgct aacctgtctt ttaacctgct tttaaaccaa tatttataaa ccttgttttt
     7141 aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc ccccccttct
     7201 cgaaccctcc cggcccgcta acgcgggcct cccatccccc caggggctgc gcccctcggc
     7261 cgcgaacggc ctcaccccaa aaatggcagc gctggcagtc cttgccattg ccgggatcgg
     7321 ggcagtaacg ggatgggcga tcagcccgag cgcgacgccc ggaagcattg acgtgccgca
     7381 ggtgctggca tcgacattca gcgaccaggt gccgggcagt gagggcggcg gcctgggtgg
     7441 cggcctgccc ttcacttcgg ccgtcggggc attcacggac ttcatggcgg ggccggcaat
     7501 ttttaccttg ggcattcttg gcatagtggt cgcgggtgcc gtgctcgtgt tcgggggtgc
     7561 gataaaccca gcgaaccatt tgaggtgata ggtaagatta taccgaggta tgaaaacgag
     7621 aattggacct ttacagaatt actctatgaa gcgccatatt taaaaagcta ccaagacgaa
     7681 gaggatgaag aggatgagga ggcagattgc cttgaatata ttgacaatac tgataagata
     7741 atatatcttt tatatagaag atatcgccgt atgtaaggat ttcagggggc aaggcatagg
     7801 cagcgcgctt atcaatatat ctatagaatg ggcaaagcat aaaaacttgc atggactaat
     7861 gcttgaaacc caggacaata accttatagc ttgtaaattc tatcataatt gggtaatgac
     7921 tccaacttat tgatagtgtt ttatgttcag ataatgcccg atgactttgt catgcagctc
     7981 caccgatttt gagaacgaca gcgacttccg tcccagccgt gccaggtgct gcctcagatt
     8041 caggttatgc cgctcaattc gctgcgtata tcgcttgctg attacgtgca gctttccctt
     8101 caggcgggat tcatacagcg gccagccatc cgtcatccat atcaccacgt caaagggtga
     8161 cagcaggctc ataagacgcc ccagcgtcgc catagtgcgt tcaccgaata cgtgcgcaac
     8221 aaccgtcttc cggagactgt catacgcgta aaacagccag cgctggcgcg atttagcccc
     8281 gacatagccc cactgttcgt ccatttccgc gcagacgatg acgtcactgc ccggctgtat
     8341 gcgcgaggtt accgactgcg gcctgagttt tttaagtgac gtaaaatcgt gttgaggcca
     8401 acgcccataa tgcgggctgt tgcccggcat ccaacgccat tcatggccat atcaatgatt
     8461 ttctggtgcg taccgggttg agaagcggtg taagtgaact gcagttgcca tgttttacgg
     8521 cagtgagagc agagatagcg ctgatgtccg gcggtgcttt tgccgttacg caccaccccg
     8581 tcagtagctg aacaggaggg acagctgata gaaacagaag ccactggagc acctcaaaaa
     8641 caccatcata cactaaatca gtaagttggc agcatcaccc ataattgtgg tttcaaaatc
     8701 ggctccgtcg atactatgtt atacgccaac tttgaaaaca actttgaaaa agctgttttc
     8761 tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta
     8821 gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa
     8881 tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg
     8941 aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa
     9001 aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga
     9061 tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg
     9121 gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag
     9181 atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact
     9241 ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg
     9301 attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa gaagacactc
     9361 catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac
     9421 ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa
     9481 gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct
     9541 gcgtccggtc gatcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact
     9601 tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt
     9661 tttagtacct agatgtggcg caacgatgcc ggcgacaagc aggagcgcac cgacttcttc
     9721 cgcatcaagt gttttggctc tcaggccgag gcccacggca agtatttggg caaggggtcg
     9781 ctggtattcg tgcagggcaa gattcggaat accaagtacg agaaggacgg ccagacggtc
     9841 tacgggaccg acttcattgc cgataaggtg gattatctgg acaccaaggc accaggcggg
     9901 tcaaatcagg aataagggca cattgccccg gcgtgagtcg gggcaatccc gcaaggaggg
     9961 tgaatgaatc ggacgtttga ccggaaggca tacaggcaag aactgatcga cgcggggttt
    10021 tccgccgagg atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc ccgcgaaacc
    10081 ttccagtccg tcggctcgat ggtccagcaa gctacggcca agatcgagcg cgacagcgtg
    10141 caactggctc cccctgccct gcccgcgcca tcggccgccg tggagcgttc gcgtcgtctc
    10201 gaacaggagg cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg aactatgacg
    10261 accaagaagc gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga ggccaagcag
    10321 gccgcgttgc tgaaacacac gaagcagcag atcaaggaaa tgcagctttc cttgttcgat
    10381 attgcgccgt ggccggacac gatgcgagcg atgccaaacg acacggcccg ctctgccctg
    10441 ttcaccacgc gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt cattttccac
    10501 gtcaacaagg acgtgaagat cacctacacc ggcgtcgagc tgcgggccga cgatgacgaa
    10561 ctggtgtggc agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga gccgatcacc
    10621 ttcacgttct acgagctttg ccaggacctg ggctggtcga tcaatggccg gtattacacg
    10681 aaggccgagg aatgcctgtc gcgcctacag gcgacggcga tgggcttcac gtccgaccgc
    10741 gttgggcacc tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga ccgtggcaag
    10801 aaaacgtccc gttgccaggt cctgatcgac gaggaaatcg tcgtgctgtt tgctggcgac
    10861 cactacacga aattcatatg ggagaagtac cgcaagctgt cgccgacggc ccgacggatg
    10921 ttcgactatt tcagctcgca ccgggagccg tacccgctca agctggaaac cttccgcctc
    10981 atgtgcggat cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg cgaagcctgc
    11041 gaagagttgc gaggcagcgg cctggtggaa cacgcctggg tcaatgatga cctggtgcat
    11101 tgcaaacgct agggccttgt ggggtcagtt ccggctgggg gttcagcagc cagcgcttta
    11161 ctggcatttc aggaacaagc gggcactgct cgacgcactt gcttcgctca gtatcgctcg
    11221 ggacgcacgg cgcgctctac gaactgccga taaacagagg attaaaattg acaattgtga
    11281 ttaaggctca gattcgacgg cttggagcgg ccgacgtgca ggatttccgc gagatccgat
    11341 tgtcggccct gaagaaagct ccagagatgt tcgggtccgt ttacgagcac gaggagaaaa
    11401 agcccatgga ggcgttcgct gaacggttgc gagatgccgt ggcattcggc gcctacatcg
    11461 acggcgagat cattgggctg tcggtcttca aacaggagga cggccccaag gacgctcaca
    11521 aggcgcatct gtccggcgtt ttcgtggagc ccgaacagcg aggccgaggg gtcgccggta
    11581 tgctgctgcg ggcgttgccg gcgggtttat tgctcgtgat gatcgtccga cagattccaa
    11641 cgggaatctg gtggatgcgc atcttcatcc tcggcgcact taatatttcg ctattctgga
    11701 gcttgttgtt tatttcggtc taccgcctgc cgggcggggt cgcggcgacg gtaggcgctg
    11761 tgcagccgct gatggtcgtg ttcatctctg ccgctctgct aggtagcccg atacgattga
    11821 tggcggtcct gggggctatt tgcggaactg cgggcgtggc gctgttggtg ttgacaccaa
    11881 acgcagcgct agatcctgtc ggcgtcgcag cgggcctggc gggggcggtt tccatggcgt
    11941 tcggaaccgt gctgacccgc aagtggcaac ctcccgtgcc tctgctcacc tttaccgcct
    12001 ggcaactggc ggccggagga cttctgctcg ttccagtagc tttagtgttt gatccgccaa
    12061 tcccgatgcc tacaggaacc aatgttctcg gcctggcgtg gctcggcctg atcggagcgg
    12121 gtttaaccta cttcctttgg ttccggggga tctcgcgact cgaacctaca gttgtttcct
    12181 tactgggctt tctcagcccc agatctgggg tcgatcagcc ggggatgcat caggccgaca
    12241 gtcggaactt cgggtccccg acctgtacca ttcggtgagc aatggatagg ggagttgata
    12301 tcgtcaacgt tcacttctaa agaaatagcg ccactcagct tcctcagcgg ctttatccag
    12361 cgatttccta ttatgtcggc atagttctca agatcgacag cctgtcacgg ttaagcgaga
    12421 aatgaataag aaggctgata attcggatct ctgcgaggga gatgatattt gatcacaggc
    12481 agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc
    12541 aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc
    12601 cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt
    12661 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat
    12721 tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg
    12781 tactggggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
    12841 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
    12901 ctgtttgatg gtggttccga aatcggcaaa atcccttata aatcaaaaga atagcccgag
    12961 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
    13021 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
    13081 aaatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
    13141 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
    13201 gcgaaaggag cgggcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    13261 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
    13321 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat taattcccat
    13381 cttgaaagaa atatagttta aatatttatt gataaaataa caagtcaggt attatagtcc
    13441 aagcaaaaac ataaatttat tgatgcaagt ttaaattcag aaatatttca ataactgatt
    13501 atatcagctg gtacattgcc gtagatgaaa gactgagtgc gatattatgt gtaatacata
    13561 aattgatgat atagctagct tagctcatcg ggggatcgat cccggtcggc atctactcta
    13621 ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac
    13681 acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc
    13741 ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt
    13801 gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag
    13861 ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat
    13921 acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa
    13981 catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt
    14041 ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat
    14101 cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca
    14161 gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc
    14221 gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat
    14281 cgcatccatg gcctccgcga ccggctgcag aacagcgggc agttcggttt caggcaggtc
    14341 ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaatgc
    14401 cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata
    14461 acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc
    14521 tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac
    14581 gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt
    14641 catatcttat tgccccccta gagtcgagat ctggattgag agtgaatatg agactctaat
    14701 tggataccga ggggaattta tggaacgtca gtggagcatt tttgacaaga aatatttgct
    14761 agctgatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga cgtatgtgct
    14821 tagctcatta aactccagaa acccgcggct gagtggctcc ttcaacgttg cggttctgtc
    14881 agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac gtgactccct
    14941 taattctccg ctcatgatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc
    15001 atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc
    15061 ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag cccactgcaa
    15121 gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca
    15181 ttcatccggg gtcagcaccg tttctgcgga ctggctttct acgtgttccg cttcctttag
    15241 cagcccttgc gccctgagtg cttgcggcag cgtgaagctt
//
