LOCUS       sequence-209395-        2116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Trigger sequence for pNP1 sfGFP toehold switch.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Huang A, Nguyen PQ, Stark JC, Takahashi MK, Donghia N, Ferrante T, 
            Dy AJ, Hsu KJ, Dubner RS, Pardee K, Jewett MC, Collins JJ
  TITLE     BioBits Explorer: A modular synthetic biology education kit.
  JOURNAL   Sci Adv. 2018 Aug 1;4(8):eaat5105. doi: 10.1126/sciadv.aat5105. 
            eCollection 2018 Aug.
  PUBMED    30083608
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-08-1"; volume: "4(8):eaat5105. doi"; pages: " 
            10.1126/sciadv.aat5105. eCollection 2018 Aug"
COMMENT     Sequence Label: pNP1 sfGFP trigger
FEATURES             Location/Qualifiers
     source          1..2116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     26..45
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      108..155
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(373..961)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(453..472)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1133..1924,1925..1993))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1756..1775
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1994..2098)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gctagtggtg ctagccccgc gaaattaata cgactcacta tagggacgtc gattcaccgt
       61 cgaatcgacg tagatacaga catacaaaga cgacaataag tgatagacta gcataacccc
      121 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggaa
      181 gcttggcact ggccgaccgg ggtcgagcac tgactcgctg cgctcggtcg ttcggctgcg
      241 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      301 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      361 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      421 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      481 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      541 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      601 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      661 cttatccggt aactatcgtc ttgagtccaa cccgctaaga cacgacttat cgccactggc
      721 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      781 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
      841 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
      901 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
      961 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacagat
     1021 ccgggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1081 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     1141 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1201 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1261 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1321 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1381 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1441 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1501 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     1561 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     1621 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     1681 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     1741 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     1801 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     1861 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     1921 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     1981 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2041 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2101 catttccccg aaaagt
//
