LOCUS       sequence-20940-e         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG304GPD-ccdB-DsRed
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(31..155)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(163..179)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(163..179)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(185..448)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(268..291)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 gaaaagcctg ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag
       61 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
      121 tacgtttctc gttcagcttt tttgtacaaa cttgtgatgg gggatccact agttctagaa
      181 tccgtcgaaa ctaagttctg gtgttttaaa actaaaaaaa agactaacta taaaagtaga
      241 atttaagaag tttaagaaat agatttacag aattacaatc aatacctacc gtctttatat
      301 acttattagt caagtagggg aataatttca gggaactggt ttcaaccttt tttttcagct
      361 ttttccaaat cagagagagc agaaggtaat agaaggtgta agaaaatgag atagatacat
      421 gcgtgggtca attgccttgt gtcatcattt actccaggca ggttgcatca ctccattgag
      481 gttgtgcccg ttttttgcct gtttgtgccc ctgttctctg tagttgcgct aagagaatgg
      541 acctatgaac tgatggttgg tgaagaaaac aatattttgg tgctgggatt cttttttttt
      601 ctggatgcca gctt
//
