LOCUS       sequence-209400-        2119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Input B for pNP1 AND gate sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Huang A, Nguyen PQ, Stark JC, Takahashi MK, Donghia N, Ferrante T, 
            Dy AJ, Hsu KJ, Dubner RS, Pardee K, Jewett MC, Collins JJ
  TITLE     BioBits Explorer: A modular synthetic biology education kit.
  JOURNAL   Sci Adv. 2018 Aug 1;4(8):eaat5105. doi: 10.1126/sciadv.aat5105. 
            eCollection 2018 Aug.
  PUBMED    30083608
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-08-1"; volume: "4(8):eaat5105. doi"; pages: " 
            10.1126/sciadv.aat5105. eCollection 2018 Aug"
COMMENT     Sequence Label: pNP1 AND input B
FEATURES             Location/Qualifiers
     source          1..2119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     26..45
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      111..158
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(376..964)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(456..475)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1136..1927,1928..1996))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1759..1778
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1997..2101)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gctagtggtg ctagccccgc gaaattaata cgactcacta tagggtcagt gccacagcag
       61 gcactgagca caattctact cctatcttta caaattacac tcataagtcc ctagcataac
      121 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      181 gaagcttggc actggccgac cggggtcgag cactgactcg ctgcgctcgg tcgttcggct
      241 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      301 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      361 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      421 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      481 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      541 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      601 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      661 cgccttatcc ggtaactatc gtcttgagtc caacccgcta agacacgact tatcgccact
      721 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      781 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      841 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      901 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      961 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcaca
     1021 gatccgggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1081 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     1141 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1201 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     1261 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     1321 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1381 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1441 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1501 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1561 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     1621 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     1681 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     1741 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     1801 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     1861 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     1921 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     1981 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     2041 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     2101 gcacatttcc ccgaaaagt
//
