LOCUS       sequence-209412-        1500 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Improved genetic encoded calcium sensor based on GCaMP6m. Also 
            called GCaMP-X.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Yang Y, Liu N, He Y, Liu Y, Ge L, Zou L, Song S, Xiong W, Liu X
  TITLE     Improved calcium sensor GCaMP-X overcomes the calcium channel 
            perturbations induced by the calmodulin in GCaMP.
  JOURNAL   Nat Commun. 2018 Apr 17;9(1):1504. doi: 10.1038/s41467-018-03719-6.
  PUBMED    29666364
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-04-17"; volume: "9(1):1504. doi"; pages: " 
            10.1038/s41467-018-03719-6"
COMMENT     Sequence Label: pN1-GCaMP6m-XC
FEATURES             Location/Qualifiers
     source          1..1500
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(112..120,121..138,139..141,142..174,175..177,178..201,
                     202..228,229..285,286..291,292..561,562..579,580..1014,
                     1015..1020,1021..1461)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6m
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKGSYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      208..217
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     498..519
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(625..646)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(886..905)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgggcatgg acgagctgta caagggtacc gctgcaacca aaattcaggc tgccttccgt
       61 ggacacataa caaggaaaaa gctcaaagac gagaagaagg gtgctgaatt catgggttct
      121 catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat gggtcgggat
      181 ctgtacgacg atgacgataa ggatctcgcc accatggtcg actcatcacg tcgtaagtgg
      241 aataagacag gtcacgcagt cagagctata ggtcggctga gctcactcga gaacgtctat
      301 atcaaggccg acaagcagaa gaacggcatc aaggcgaact tcaagatccg ccacaacatc
      361 gaggacggcg gcgtgcagct cgcctaccac taccagcaga acacccccat cggcgacggc
      421 cccgtgctgc tgcccgacaa ccactacctg agcgtgcagt ccaaactttc gaaagacccc
      481 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      541 ggcatggacg agctgtacaa gggcggtacc ggagggagca tggtgagcaa gggcgaggag
      601 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      661 ttcagcgtgt ccggcgaggg tgagggcgat gccacctacg gcaagctgac cctgaagttc
      721 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
      781 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      841 gccatgcccg aaggctacat ccaggagcgc accatcttct tcaaggacga cggcaactac
      901 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      961 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacctgccg
     1021 gaccaactga ctgaagagca gatcgcagaa tttaaagagg ctttctccct atttgacaag
     1081 gacggggatg ggacaataac aaccaaggag ctggggacgg tgatgcggtc tctggggcag
     1141 aaccccacag aagcagagct gcaggacatg atcaatgaag tagatgccga cggtgacggc
     1201 acaatcgact tccctgagtt cctgacaatg atggcaagaa aagggagcta cagggacacg
     1261 gaagaagaaa ttagagaagc gttcggtgtg tttgataagg atggcaatgg ctacatcagt
     1321 gcagcagagc ttcgccacgt gatgacaaac cttggagaga agttaacaga tgaagaggtt
     1381 gatgaaatga tcagggaagc agacatcgat ggggatggtc aggtaaacta cgaagagttt
     1441 gtacaaatga tgacagcgaa gcttgctctt aagttggctg gacttgatat tggttcttaa
//
