LOCUS       sequence-209420-       10010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of Paxillin-mTurqoise.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10010)
  AUTHORS   Kenific CM, Stehbens SJ, Goldsmith J, Leidal AM, Faure N, Ye J, 
            Wittmann T, Debnath J
  TITLE     NBR1 enables autophagy-dependent focal adhesion turnover.
  JOURNAL   J Cell Biol. 2016 Feb 29;212(5):577-590. Epub 2016 Feb 22.
  PUBMED    26903539
REFERENCE   2  (bases 1 to 10010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2016-02-29"; volume: "212(5)"; pages: "577-590. Epub 
            2016 Feb 22"
COMMENT     Sequence Label: pLentiblast-Paxillin-mTurqoise
FEATURES             Location/Qualifiers
     source          1..10010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1919
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1965..2475
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2394..2412
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    4164..4216
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      4224..4233
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4230..4232,4233..4235,4236..4949)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4275..4296)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4536..4555)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4883..4904
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4957..5545
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5010..5030)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5428..5439)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5596..5616)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5601..5930
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5781..5916
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5978..6025
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6044..6442
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6529..6762
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6834..6955
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6871..6890)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6925..6944
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6995..7130
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7150..7169)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7151..7169)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7179..7196)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7179..7195)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7188..7210)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7337..7792
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7424..7443)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7634..7655
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7818..7922
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7923..7991,7992..8783)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8141..8160)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8954..9542
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9443..9462
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9696..9713
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9830..9851
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9866..9871,9872..9889,9890..9896)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9904..9920
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9909..9931
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9928..9944
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9928..9944
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9963..9983
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9965..9983
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta
     1921 tcgatcacga gactagcctc gagaagcttg atatcgaatt ccacggggtt ggggttgcgc
     1981 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
     2041 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
     2101 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
     2161 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
     2221 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
     2281 gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg
     2341 gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc
     2401 gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc
     2461 gacctctctc cccaggggga tctaccatgg acgacctcga tgccttactg gcagacctgg
     2521 aatccaccac ctcgcatatc tctaagcgac cggtgtttct aacagaggaa acgccttact
     2581 cctacccaac tggaaaccat acgtaccagg agattgctgt gccaccgccg gtgcccccgc
     2641 cgccttccag tgaggcccta aatggcactg tgattgaccc cttagaccag tggcaaccca
     2701 gcgtgtccag atacggccac cagcaacctc agtcccagtc tccgatatac agctctagtg
     2761 ccaaaagctc cagtgcctct gttcctagag acgggctcag ctccccttct ccccgtgcca
     2821 gtgaggagga acacgtgtac agcttcccaa acaagcagaa gtctgcagaa ccatctccca
     2881 caatgaccag cacctccctg ggcagcaacc tctcggaact ggacaggctt ctcctggaac
     2941 tgaatgctgt tcaacataat ccccccagtg gcttctcagc agatgaagtc agcagaagcc
     3001 catcactgcc caatgtgacc ggacctcact atgtcatccc agagagcagc agctctgcag
     3061 gagggaaggc tgcaccccct acaaaagaaa aaccaaagcg gaatggtggg cgtgggattg
     3121 aagatgtgcg tcccagtgtg gagagcctgc tggatgagct ggagagctct gtgccaagcc
     3181 cagtccctgc aatcactgtg agccaagggg aggtgagcag cccccagcgt gtcaacgcca
     3241 gtcagcagca gacccgtatc tctgcttctt cagctacacg agaactggat gagctgatgg
     3301 cgtccctctc tgactttaag ttcatggcac aggggaaagc cggcggcagc agctccccac
     3361 catccaccac cccaaagcct ggcagtcagc tggacaccat gctgggaagt ctgcagtctg
     3421 acctgaacaa actgggggta gcgacggtcg ccaagggtgt gtgcggagcc tgcaagaagc
     3481 cgattgctgg gcaggtagtt acagccatgg ggaagacctg gcaccctgag cacttcgtct
     3541 gcacccactg ccaggaggag attggatcac ggaacttctt tgagcgggat gggcagccct
     3601 actgtgagaa ggactatcac aacctcttct ctcctcgctg ctactactgc aatgggccaa
     3661 tccttgataa agtggtgacg gctttggaca ggacgtggca ccctgaacac tttttctgtg
     3721 cccagtgtgg agttttcttt ggacctgaag gatttcatga gaaggatggc aaagcctatt
     3781 gccgcaagga ctactttgac atgtttgctc ccaagtgtgg aggctgtgcc cgggctatcc
     3841 tggaaaatta catttctgcc ttaaacaccc tgtggcaccc tgagtgcttt gtctgtcggg
     3901 aatgtttcac accgttcatc aacggcagct tcttcgagca cgacgggcag ccctactgcg
     3961 aggtgcatta ccacgagcgc cgcggctcgc tgtgctctgg ctgccagaag cccatcacgg
     4021 gacgctgcat cactgccatg ggcaagaaat ttcaccccga acactttgtc tgtgccttct
     4081 gcctcaagca gctcaacaaa ggaaccttca aagaacagaa cgacaagccc tactgtcaga
     4141 actgctttct caaactcttc tgtctcgagc tcaagcttcg aattctgcag tcgacggtac
     4201 cgcgggcccg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
     4261 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     4321 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     4381 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgtcctgg ggcgtgcagt
     4441 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     4501 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     4561 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     4621 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc agcgacaacg
     4681 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca
     4741 acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     4801 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag
     4861 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     4921 ctctcggcat ggacgagctg tacaagtaag ctcgacaatc aacctctgga ttacaaaatt
     4981 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     5041 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     5101 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     5161 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     5221 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     5281 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     5341 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     5401 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     5461 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     5521 atctcccttt gggccgcctc cccgcctgga attctgcaga tatccggtta gtaatgagtt
     5581 tggaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     5641 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     5701 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     5761 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5821 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     5881 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     5941 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     6001 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agcctttgtc
     6061 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
     6121 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
     6181 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
     6241 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
     6301 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
     6361 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
     6421 tggttatgtg tgggagggct aagcacaatt cgagctcggt acctttaaga ccaatgactt
     6481 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctag
     6541 ctcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     6601 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     6661 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     6721 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     6781 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     6841 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6901 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6961 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     7021 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7081 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     7141 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     7201 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     7261 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     7321 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     7381 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     7441 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     7501 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     7561 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     7621 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     7681 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     7741 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     7801 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7861 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7921 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7981 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     8041 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     8101 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     8161 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     8221 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     8281 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     8341 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     8401 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     8461 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8521 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8581 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8641 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8701 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8761 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8821 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8881 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8941 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     9001 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     9061 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     9121 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     9181 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     9241 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9301 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     9361 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     9421 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9481 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9541 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     9601 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     9661 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     9721 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     9781 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     9841 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     9901 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     9961 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
//
