LOCUS       sequence-209421-         633 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRT integration of beta-globin reporter with premature termination 
            codon (PTC39); enables detection of 5'-3' decay intermediates 
            (xrFrag) and allows detection via northern blot (4H binding sites).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Boehm V, Gerbracht JV, Marx MC, Gehring NH
  TITLE     Interrogating the degradation pathways of unstable mRNAs with 
            XRN1-resistant sequences.
  JOURNAL   Nat Commun. 2016 Dec 5;7:13691. doi: 10.1038/ncomms13691.
  PUBMED    27917860
REFERENCE   2  (bases 1 to 633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-12-5"; volume: "7:13691. doi"; pages: " 
            10.1038/ncomms13691"
COMMENT     Sequence Label: pcDNA5/FRT/TO-globin_del5UTR_PTC39-xrRNA-4H
FEATURES             Location/Qualifiers
     source          1..633
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctctgcggcc gcccgggtcg acaaattgga cagcaagaaa gcgagcttag tgatacttgt
       61 gggccagggc attagccaca ccagccacca ctttctgata ggcagcctgc actggtgggg
      121 tgctcgacaa attggacagc aagaaagcga gcttagtgat acttgtgggc cagggcatta
      181 gccacaccag ccaccacttt ctgataggca gcctgcactg gtggggtgct cgacaaattg
      241 gacagcaaga aagcgagctt agtgatactt gtgggccagg gcattagcca caccagccac
      301 cactttctga taggcagcct gcactggtgg ggtgctcgac aaattggaca gcaagaaagc
      361 gagcttagtg atacttgtgg gccagggcat tagccacacc agccaccact ttctgatagg
      421 cagcctgcac tggtggggtg ctcgactgtt tacccagtcc tcctggggcc tcacagattg
      481 cactcctcgg cgaagtggcg ctttcgcgat ctgacacggg cttcatctcc attgttggca
      541 gggactcctc ttccgagacg gttctgggag gctttccaac cgtggagaat cagctttggt
      601 aacccagtcc tcctggggtt ggtcgcaggc agc
//
