LOCUS       sequence-20943-e         664 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG305GAL-ccdB-DsRed
FEATURES             Location/Qualifiers
     source          1..664
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(22..146)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(154..170)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(154..170)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(185..626)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(191..214)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    509..626
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(645..664)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(646..664)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggggtgcct aatgcggccg ccatagtgac tggatatgtt gtgttttaca gtattatgta
       61 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct
      121 cgttcagctt ttttgtacaa acttgtgatg ggggatccac tagttctaga atccggggtt
      181 ttttctcctt gacgttaaag tatagaggta tattaacaat tttttgttga tacttttatt
      241 acatttgaat aagaagtaat acaaaccgaa aatgttgaaa gtattagtta aagtggttat
      301 gcagtttttg catttatata tctgttaata gatcaaaaat catcgcttcg ctgattaatt
      361 accccagaaa taaggctaaa aaactaatcg cattatcatc ctatggttgt taatttgatt
      421 cgttcatttg aaggtttgtg gggccaggtt actgccaatt tttcctcttc ataaccataa
      481 aagctagtat tgtagaatct ttattgttcg gagcagtgcg gcgcgaggca catctgcgtt
      541 tcaggaacgc gaccggtgaa gacgaggacg cacggaggag agtcttcctt cggagggctg
      601 tcacccgctc ggcggcttct aatccgtact agacctccaa ttcgccctat agtgagtcgt
      661 atta
//
