LOCUS       sequence-209467-        2859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the expression of the construct SNCA-5Asp. 
            Alpha-Synuclein with 6 aa C-terminal moiety, which consists of 2 Asn
            and 4 Gly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Afitska K, Fucikova A, Shvadchak VV, Yushchenko DA
  TITLE     Modification of C Terminus Provides New Insights into the Mechanism 
            of alpha-Synuclein Aggregation.
  JOURNAL   Biophys J. 2017 Nov 21;113(10):2182-2191. doi: 
            10.1016/j.bpj.2017.08.027. Epub 2017 Sep 20.
  PUBMED    28939194
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2017.08.027"; journalName: "Biophys J"; date: 
            "2017-11-21- 21"; volume: "113"; issue: "10"; pages: "2182-2191"
COMMENT     Sequence Label: r-6. pT7-7-SNCA-2Asn
FEATURES             Location/Qualifiers
     source          1..2859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     132..151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     385..402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     637..659
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     724..743
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        724..742
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             774..796
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(join(1322..2113,2114..2182))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1945..1964
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2183..2287)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2355..2373
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      join(2502..2859,1..231)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
       61 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
      421 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatagg aagatcttcc
      481 ggaagatctt cctatggtgc actctcagta caatctgctc tgatgcgcta cgtgactggg
      541 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      601 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      661 tttcaccgtc atcaccgaaa cgcgcgaggc ccagcgattc gaacttctga tagacttcga
      721 aattaatacg actcactata gggagaccac aacggtttcc ctctagaaat aattttgttt
      781 aactttaaga aggagatata catatggatg tattcatgaa aggactttca aaggccaagg
      841 agggagttgt ggctgctgct gagaaaacca aacagggtgt ggcagaagca gcaggaaaga
      901 caaaagaggg tgttctctat gtaggctcca aaaccaagga gggagtggtg catggtgtgg
      961 caacagtggc tgagaagacc aaagagcaag tgacaaatgt tggaggagca gtggtgacgg
     1021 gtgtgacagc agtagcccag aagacagtgg agggagcagg gagcattgca gcagccactg
     1081 gctttgtcaa aaaggaccag ttgggcaaga atgaagaagg agccccacag gaaggaattc
     1141 tggaagatat gcctgtggat cctgacaatg aggcttatga aatgccttct gaggaagggt
     1201 atcaagacta tgaacctgaa gccggcggca acggcaacgg ctaaaagctt atcgatgata
     1261 agctgtcaaa catgagaatt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1321 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1381 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1441 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     1501 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1561 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1621 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1681 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1741 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1801 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     1861 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     1921 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     1981 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     2041 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     2101 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2161 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     2221 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2281 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     2341 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa taaaaggatc
     2401 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2461 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2521 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2581 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2641 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2701 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2761 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2821 acggggggtt cgtgcacaca gcccagcttg gagcgaacg
//
