LOCUS       sequence-209490-        2877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the expression of the construct SNCA-5Lys. 
            Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 
            Lys and 7 Gly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Afitska K, Fucikova A, Shvadchak VV, Yushchenko DA
  TITLE     Modification of C Terminus Provides New Insights into the Mechanism 
            of alpha-Synuclein Aggregation.
  JOURNAL   Biophys J. 2017 Nov 21;113(10):2182-2191. doi: 
            10.1016/j.bpj.2017.08.027. Epub 2017 Sep 20.
  PUBMED    28939194
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2017.08.027"; journalName: "Biophys J"; date: 
            "2017-11-21- 21"; volume: "113"; issue: "10"; pages: "2182-2191"
COMMENT     Sequence Label: t2. pT7-7-SNCA-5Lys
FEATURES             Location/Qualifiers
     source          1..2877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     132..151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     385..402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     637..659
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     724..743
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        724..742
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             774..796
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(join(1340..2131,2132..2200))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1963..1982
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2201..2305)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2373..2391
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      join(2520..2877,1..231)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
       61 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
      421 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatagg aagatcttcc
      481 ggaagatctt cctatggtgc actctcagta caatctgctc tgatgcgcta cgtgactggg
      541 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      601 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      661 tttcaccgtc atcaccgaaa cgcgcgaggc ccagcgattc gaacttctga tagacttcga
      721 aattaatacg actcactata gggagaccac aacggtttcc ctctagaaat aattttgttt
      781 aactttaaga aggagatata catatggatg tattcatgaa aggactttca aaggccaagg
      841 agggagttgt ggctgctgct gagaaaacca aacagggtgt ggcagaagca gcaggaaaga
      901 caaaagaggg tgttctctat gtaggctcca aaaccaagga gggagtggtg catggtgtgg
      961 caacagtggc tgagaagacc aaagagcaag tgacaaatgt tggaggagca gtggtgacgg
     1021 gtgtgacagc agtagcccag aagacagtgg agggagcagg gagcattgca gcagccactg
     1081 gctttgtcaa aaaggaccag ttgggcaaga atgaagaagg agccccacag gaaggaattc
     1141 tggaagatat gcctgtggat cctgacaatg aggcttatga aatgccttct gaggaagggt
     1201 atcaagacta tgaacctgaa gccggcggca aaggcaaagg caaaggcaaa ggcaaaggct
     1261 aaaagcttat cgatgataag ctgtcaaaca tgagaattaa atcaatctaa agtatatatg
     1321 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     1381 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     1441 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     1501 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1561 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1621 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1681 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1741 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1801 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1861 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1921 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     1981 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     2041 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     2101 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     2161 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     2221 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     2281 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     2341 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     2401 ttcaagaata aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     2461 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2521 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2581 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2641 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     2701 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2761 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2821 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacg
//
