LOCUS       sequence-209502-       10203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter of Erk activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10203)
  AUTHORS   Dine E, Gil AA, Uribe G, Brangwynne CP, Toettcher JE
  TITLE     Protein Phase Separation Provides Long-Term Memory of Transient 
            Spatial Stimuli.
  JOURNAL   Cell Syst. 2018 May 24. pii: S2405-4712(18)30190-X. doi: 
            10.1016/j.cels.2018.05.002.
  PUBMED    29859829
REFERENCE   2  (bases 1 to 10203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 May 24. pii: S2405-4712(18)30190-X. doi: 
            10.1016/j.cels.2018.05.002."
COMMENT     Sequence Label: ErkKTR-irFP
FEATURES             Location/Qualifiers
     source          1..10203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             249..293
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    576..693
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        847..1255
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      1594..1603
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1600..2547
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     misc_feature    2645..3233
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2698..2718)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3116..3127)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3296..3529
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3554..3572)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3555..3572)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3862..3881)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4215..4237
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4275..4293)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4361..4465
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4466..4534,4535..5326)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4684..4703)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5497..6085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5986..6005
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6239..6256
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6326..6346)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6331..6660
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6511..6646
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7794..7859
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7989..8009
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8434..8568
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8471..8490)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8525..8544
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8737..9370
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9417..9542
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(10034..10203,1..64)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
       61 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
      121 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
      181 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
      241 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
      301 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
      361 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
      421 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
      481 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
      541 ctcgacggtc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
      601 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
      661 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
      721 ccagtttgga tcgataagct tgatatcgaa ttcctgcagc cccgataaaa taaaagattt
      781 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggtta tggcaagcta
      841 gctgcagtaa cgccattatt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt
      901 cagatcaagg gcgggtacat gaaaatagct aacgtagggc caaacaggat atctgcggtg
      961 agcagtttcg gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc
     1021 ccgaggccaa gagcagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc
     1081 catcagatgt ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta
     1141 accaatcagc ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag
     1201 agctcacaac ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccggggggga
     1261 tctggagctc tcgagaattc tcacgcgtca agtggagcaa ggcaggtgga cagtggatcc
     1321 ggctccacca tgaagggccg aaagcctcgg gacctggaac ttccacttag cccaagcctg
     1381 ctgggtggcc agggacctga acggactcca ggatcaggaa caagctctgg tcttcaggca
     1441 ccggggccag cgctttcgcc cagcaagcgt agcggtctcg aggacccagc gacgccatcg
     1501 aagaagccac gtaccccatc ggtgtcgtca aggctggagc gcctgaccct ccagtccagc
     1561 tttcaattcc catccactag tccggatccg gtcgccacca tggctgaagg ctccgtcgcc
     1621 aggcagcctg acctcttgac ctgcgacgat gagccgatcc atatccccgg tgccatccaa
     1681 ccgcatggac tgctgctcgc cctcgccgcc gacatgacga tcgttgccgg cagcgacaac
     1741 cttcccgaac tcaccggact ggcgatcggc gccctgatcg gccgctctgc ggccgatgtc
     1801 ttcgactcgg agacgcacaa ccgtctgacg atcgccttgg ccgagcccgg ggcggccgtc
     1861 ggagcaccga tcactgtcgg cttcacgatg cgaaaggacg caggcttcat cggctcctgg
     1921 catcgccatg atcagctcat cttcctcgag ctcgagcctc cccagcggga cgtcgccgag
     1981 ccgcaggcgt tcttccgccg caccaacagc gccatccgcc gcctgcaggc cgccgaaacc
     2041 ttggaaagcg cctgcgccgc cgcggcgcaa gaggtgcgga agattaccgg cttcgatcgg
     2101 gtgatgatct atcgcttcgc ctccgacttc agcggcgaag tgatcgcaga ggatcggtgc
     2161 gccgaggtcg agtcaaaact aggcctgcac tatcctgcct caaccgtgcc ggcgcaggcc
     2221 cgtcggctct ataccatcaa cccggtacgg atcattcccg atatcaatta tcggccggtg
     2281 ccggtcaccc cagacctcaa tccggtcacc gggcggccga ttgatcttag cttcgccatc
     2341 ctgcgcagcg tctcgcccgt ccatctggaa ttcatgcgca acataggcat gcacggcacg
     2401 atgtcgatct cgattttgcg cggcgagcga ctgtggggat tgatcgtttg ccatcaccga
     2461 acgccgtact acgtcgatct cgatggccgc caagcctgcg agctagtcgc ccaggttctg
     2521 gcctggcaga tcggcgtgat ggaagaggcg gccgcaactc ccacctgcaa catgcgtgac
     2581 tgactgaggc cgcgactcta gagtcgacct gcaggcatgc aagcttgata tcaagcttat
     2641 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     2701 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     2761 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     2821 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     2881 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     2941 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     3001 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     3061 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     3121 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     3181 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     3241 ccgtcgacca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     3301 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     3361 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     3421 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     3481 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagaat
     3541 taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt
     3601 attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct
     3661 tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac
     3721 gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca
     3781 gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg
     3841 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
     3901 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
     3961 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
     4021 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa
     4081 tggtgcactc tcagtacaat ctagctctga tgccgcatag ttaagccagc cccgacaccc
     4141 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     4201 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     4261 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     4321 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4381 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4441 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4501 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4561 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4621 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4681 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     4741 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4801 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4861 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4921 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4981 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5041 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5101 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5161 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5221 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5281 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5341 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5401 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5461 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5521 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5581 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5641 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5701 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5761 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5821 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5881 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5941 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6001 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6061 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6121 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6181 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     6241 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     6301 ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     6361 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     6421 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6481 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     6541 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     6601 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     6661 aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc
     6721 agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc
     6781 agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa
     6841 acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat
     6901 ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc
     6961 cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat
     7021 gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg
     7081 aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt
     7141 ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca
     7201 aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg
     7261 attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct
     7321 tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt
     7381 tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt
     7441 caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc
     7501 acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat
     7561 taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc
     7621 acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg
     7681 ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact
     7741 tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata
     7801 taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga
     7861 ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc
     7921 tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt
     7981 ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc
     8041 taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca
     8101 ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct
     8161 ttataagtag gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc
     8221 atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt
     8281 gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc
     8341 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     8401 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     8461 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8521 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
     8581 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
     8641 ctagtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
     8701 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
     8761 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     8821 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     8881 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     8941 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     9001 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     9061 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     9121 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     9181 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     9241 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     9301 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     9361 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     9421 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     9481 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     9541 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     9601 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     9661 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     9721 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     9781 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     9841 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggtgat cttcagacct
     9901 ggacgatata tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     9961 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
    10021 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
    10081 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
    10141 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
    10201 ggg
//
