LOCUS       sequence-209503-       13183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For optogenetic clustering of FGFR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13183)
  AUTHORS   Dine E, Gil AA, Uribe G, Brangwynne CP, Toettcher JE
  TITLE     Protein Phase Separation Provides Long-Term Memory of Transient 
            Spatial Stimuli.
  JOURNAL   Cell Syst. 2018 May 24. pii: S2405-4712(18)30190-X. doi: 
            10.1016/j.cels.2018.05.002.
  PUBMED    29859829
REFERENCE   2  (bases 1 to 13183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 May 24. pii: S2405-4712(18)30190-X. doi: 
            10.1016/j.cels.2018.05.002."
COMMENT     Sequence Label: FGFR1-optoDroplet
FEATURES             Location/Qualifiers
     source          1..13183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        312..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             754..774
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             1179..1190
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1450..2145
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     CDS             2164..3657
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    5090..5678
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5143..5163)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5561..5572)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5741..5974
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5999..6017)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6000..6017)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6307..6326)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     6660..6682
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6720..6738)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6806..6910
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6911..6979,6980..7771)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7129..7148)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7942..8530
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8431..8450
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8684..8701
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8771..8791)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8776..9105
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8956..9091
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10239..10304
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10434..10454
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10879..11013
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10916..10935)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10970..10989
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11182..11815
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11862..11987
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12479..12712
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12897..12941
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
      241 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggttatggca
      301 agctagctgc agtaacgcca ttattgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt agggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagagca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg cgtatgggtt cctccaaatc caagcccaag
      781 gacgcatctc agcgacgtcg cgcctcaaac gattataccc aacaagcaac ccaaagctat
      841 ggggcctacc ccacccagcc cgggcagggc tattcccagc agagcagtca gccctacgga
      901 cagcagagtt acagtggtta tagccagtcc acggacactt caggctatgg ccagagcagc
      961 tattcttctt atggccagag ccagaacaca ggctatggaa ctcagtcaac tccccaggga
     1021 tatggctcga ctggcggcta tggcagtagc cagagctccc aatcgtctta cgggcagcag
     1081 tcctcctatc ctggctatgg ccagcagcca gctcccagca gcacctcggg aagttacggt
     1141 agcagttctc agagcagcag ctatgggcag ccccagagtg ggagctacag ccagcagcct
     1201 agctatggtg gacagcagca aagctatgga cagcagcaaa gctataatcc ccctcagggc
     1261 tatggacagc agaaccagta caacagcagc agtggtggtg gaggtggagg tggaggtgga
     1321 ggtaactatg gccaagatca atcctccatg agtagtggtg gtggcagtgg tggcggttat
     1381 ggcaatcaag accagagtgg tggaggtggc agcggtggct atggacagca ggaccgtgga
     1441 ggtggaggaa tggtgagcga gctgattaag gagaacatgc ccatgaagct gtacatggag
     1501 ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
     1561 ggcacccaga ccatgagaat caaggtcgtc gagggcggcc ctctcccctt cgccttcgac
     1621 atcctggcta ccagcttcat gtacggcagc agaaccttca tcaagcaccc tccgggcatc
     1681 cccgacttct ttaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
     1741 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     1801 tacaacgtca aggttagagg ggtgaacttc ccagccaacg gccctgtgat gcagaagaaa
     1861 acactcggct gggaggcctc caccgagacg atgtaccccg ctgacggcgg cctggaaggc
     1921 gcatgtgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa ccttgagacc
     1981 acatacagat ccaagaaacc cgctacgaac ctcaagatgc ccggcgtcta caacgtggac
     2041 cacagactgg aaagaatcaa ggaggccgac gatgagacct acgtcgagca gcacgaggtg
     2101 gctgtggcca gatactctac tggtggcgct ggtgatggag gtaaagcggc cacgcgtacg
     2161 cgtatgaaga tggacaaaaa gactatagtt tggtttagaa gagacctaag gattgaggat
     2221 aatcctgcat tagcagcagc tgctcacgaa ggatctgttt ttcctgtctt catttggtgt
     2281 cctgaagaag aaggacagtt ttatcctgga agagcttcaa gatggtggat gaaacaatca
     2341 cttgctcact tatctcaatc cttgaaggct cttggatctg acctcacttt aatcaaaacc
     2401 cacaacacga tttcagcgat cttggattgt atccgcgtta ccggtgctac aaaagtcgtc
     2461 tttaaccacc tctatgatcc tgtttcgtta gttcgggacc ataccgtaaa ggagaagctg
     2521 gtggaacgtg ggatctctgt gcaaagctac aatggagatc tattgtatga accgtgggag
     2581 atatactgcg aaaagggcaa accttttacg agtttcaatt cttactggaa gaaatgctta
     2641 gatatgtcga ttgaatccgt tatgcttcct cctccttggc ggttgatgcc aataactgca
     2701 gcggctgaag cgatttgggc gtgttcgatt gaagaactag ggctggagaa tgaggccgag
     2761 aaaccgagca atgcgttgtt aactagagct tggtctccag gatggagcaa tgctgataag
     2821 ttactaaatg agttcatcga gaagcagttg atagattatg caaagaacag caagaaagtt
     2881 gttgggaatt ctacttcact actttctccg tatctccatt tcggggaaat aagcgtcaga
     2941 cacgttttcc agtgtgcccg gatgaaacaa attatatggg caagagataa gaacagtgaa
     3001 ggagaagaaa gtgcagatct ttttcttagg ggaatcggtt taagagagta ttctcggtat
     3061 atatgtttca acttcccgtt tactcacgag caatcgttgt tgagtcatct tcggtttttc
     3121 ccttgggatg ctgatgttga taagttcaag gcctggagac aaggcaggac cggttatccg
     3181 ttggtggatg ccggaatgag agagctttgg gctaccggat ggatgcataa cagaataaga
     3241 gtgattgttt caagctttgc tgtgaagttt cttctccttc catggaaatg gggaatgaag
     3301 tatttctggg atacactttt ggatgctgat ttggaatgtg acatccttgg ctggcagtat
     3361 atctctggga gtatccccga tggccacgag cttgatcgct tggacaatcc cgcgttacaa
     3421 ggcgccaaat atgacccaga aggtgagtac ataaggcaat ggcttcccga gcttgcgaga
     3481 ttgccaactg aatggatcca tcatccatgg gacgctcctt taaccgtact caaagcttct
     3541 ggtgtggaac tcggaacaaa ctatgcgaaa cccattgtag acatcgacac agctcgtgag
     3601 ctactagcta aagctatttc aagaacccgt gaagcacaga tcatgatcgg agcagcagcc
     3661 cgggatccac cggtcgccac cggttcaggg agcggatccg gctcagggag tgcggccgac
     3721 aagatgaaga gtggtaccaa gaagagtgac ttccacagcc agatggctgt gcacaagctg
     3781 gccaagagca tccctctgcg cagacaggtg tctgctgact ccagtgcatc catgaactct
     3841 ggggttcttc tggttcggcc atcacggctc tcctccagtg ggactcccat gctagcaggg
     3901 gtctctgagt atgagcttcc cgaagaccct cgctgggagc tgcctcggga cagactggtc
     3961 ttaggcaaac ccctgggaga gggctgcttt gggcaggtgg tgttggcaga ggctatcggg
     4021 ctggacaagg acaaacccaa ccgtgtgacc aaagtggctg tgaagatgtt gaagtcggac
     4081 gcaacagaga aagacttgtc agacctgatc tcagaaatgg agatgatgaa gatgatcggg
     4141 aagcataaga atatcatcaa cctgctgggg gcctgcacgc aggatggtcc cttgtatgtc
     4201 atcgtggagt atgcctccaa gggcaacctg cgggagtacc tgcaggcccg gaggccccca
     4261 gggctggaat actgctacaa ccccagccac aacccagagg agcagctctc ctccaaggac
     4321 ctggtgtcct gcgcctacca ggtggcccga ggcatggagt atctggcctc caagaagtgc
     4381 atacaccgag acctggcagc caggaatgtc ctggtgacag aggacaatgt gatgaagata
     4441 gcagactttg gcctcgcacg ggacattcac cacatcgact actataaaaa gacaaccaac
     4501 ggccgactgc ctgtgaagtg gatggcaccc gaggcattat ttgaccggat ctacacccac
     4561 cagagtgatg tgtggtcttt cggggtgctc ctgtgggaga tcttcactct gggcggctcc
     4621 ccataccccg gtgtgcctgt ggaggaactt ttcaagctgc tgaaggaggg tcaccgcatg
     4681 gacaagccca gtaactgcac caacgagctg tacatgatga tgcgggactg ctggcatgca
     4741 gtgccctcac agagacccac cttcaagcag ctggtggaag acctggaccg catcgtggcc
     4801 ttgacctcca accaggagta cctggacctg tccatgcccc tggaccagta ctcccccagc
     4861 tttcccgaca cccggagctc tacgtgctcc tcaggggagg attccgtctt ctctcatgag
     4921 ccgctgcccg aggagccctg cctgccccga cacccagccc agcttgccaa tggcggactc
     4981 aaacgccgct gagcggccgc aactcccacc tgcaacatgc gtgactgact gaggccgcga
     5041 ctctagagtc gacctgcagg catgcaagct tgatatcaag cttatcgata atcaacctct
     5101 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     5161 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     5221 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     5281 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     5341 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     5401 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     5461 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     5521 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     5581 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     5641 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gaccaaggca
     5701 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     5761 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     5821 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     5881 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     5941 agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc
     6001 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
     6061 gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc
     6121 tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt
     6181 ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta
     6241 gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
     6301 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
     6361 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
     6421 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
     6481 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt
     6541 acaatctagc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     6601 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     6661 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     6721 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     6781 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     6841 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     6901 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     6961 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7021 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7081 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7141 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7201 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7261 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7321 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7381 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7441 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7501 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7561 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7621 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7681 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7741 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7801 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     7861 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7921 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7981 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8041 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     8101 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8161 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8221 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8281 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8341 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8401 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8461 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8521 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8581 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     8641 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     8701 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     8761 atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     8821 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     8881 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     8941 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     9001 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     9061 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag
     9121 acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc
     9181 gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct
     9241 cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact
     9301 tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact
     9361 cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg
     9421 tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt
     9481 atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc
     9541 gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg
     9601 attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt
     9661 ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg
     9721 gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca
     9781 ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct
     9841 ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa
     9901 acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca
     9961 gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg
    10021 gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag
    10081 cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa
    10141 ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat
    10201 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
    10261 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
    10321 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
    10381 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
    10441 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
    10501 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
    10561 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
    10621 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
    10681 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
    10741 aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
    10801 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
    10861 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    10921 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    10981 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
    11041 catcccaaac ttcccacccc ataccctatt accactgcca attacctagt ggtttcattt
    11101 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
    11161 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
    11221 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
    11281 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
    11341 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
    11401 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
    11461 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
    11521 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
    11581 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
    11641 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
    11701 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
    11761 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
    11821 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
    11881 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
    11941 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
    12001 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
    12061 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
    12121 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
    12181 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
    12241 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
    12301 aaagtaagac caccgcacag caagcggccg gtgatcttca gacctggacg atatatatga
    12361 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
    12421 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
    12481 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
    12541 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
    12601 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
    12661 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
    12721 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
    12781 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
    12841 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
    12901 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
    12961 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
    13021 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
    13081 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
    13141 aaggtggaga gagagacaga gacagatcca ttcgattagt gaa
//
