LOCUS       sequence-209505-       11508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For PixELL system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11508)
  AUTHORS   Dine E, Gil AA, Uribe G, Brangwynne CP, Toettcher JE
  TITLE     Protein Phase Separation Provides Long-Term Memory of Transient 
            Spatial Stimuli.
  JOURNAL   Cell Syst. 2018 May 24. pii: S2405-4712(18)30190-X. doi: 
            10.1016/j.cels.2018.05.002.
  PUBMED    29859829
REFERENCE   2  (bases 1 to 11508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 May 24. pii: S2405-4712(18)30190-X. doi: 
            10.1016/j.cels.2018.05.002."
COMMENT     Sequence Label: FUS-Citrine-PixE
FEATURES             Location/Qualifiers
     source          1..11508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             219..263
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    546..663
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        817..1225
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1680..1691
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             join(1951..1953,1954..1956,1957..2667)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1996..2017)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2257..2276)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2604..2625
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3920..4508
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3973..3993)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4391..4402)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4571..4804
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4829..4847)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4830..4847)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5137..5156)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     5490..5512
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5550..5568)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5636..5740
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5741..5809,5810..6601)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5959..5978)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6772..7360
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7261..7280
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7514..7531
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7601..7621)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7606..7935
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7786..7921
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9069..9134
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9264..9284
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9709..9843
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9746..9765)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9800..9819
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10012..10645
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10692..10817
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(11309..11508,1..34)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
       61 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
      121 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
      181 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
      241 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
      301 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
      361 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
      421 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
      481 gagacagatc cattcgatta gtgaacggat ctcgacggtc gccaaatggc agtattcatc
      541 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
      601 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
      661 tttcgggttt attacaggga cagcagagat ccagtttgga tcgataagct tgatatcgaa
      721 ttcctgcagc cccgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
      781 aagaccccac ctgtaggtta tggcaagcta gctgcagtaa cgccattatt gcaaggcatg
      841 gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg gcgggtacat gaaaatagct
      901 aacgtagggc caaacaggat atctgcggtg agcagtttcg gccccggccc ggggccaaga
      961 acagatggtc accgcagttt cggccccggc ccgaggccaa gagcagatgg tccccagata
     1021 tggcccaacc ctcagcagtt tcttaagacc catcagatgt ttccaggctc ccccaaggac
     1081 ctgaaatgac cctgcgcctt atttgaatta accaatcagc ctgcttctcg cttctgttcg
     1141 cgcgcttctg cttcccgagc tctataaaag agctcacaac ccctcactcg gcgcgccagt
     1201 cctccgacag actgagtcgc ccggggggga tctggagctc tcgagaattc tcacgcgtca
     1261 agtggagcaa ggcaggtgga cagtggatcc ggagctacca tggcctcaaa cgattatacc
     1321 caacaagcaa cccaaagcta tggggcctac cccacccagc ccgggcaggg ctattcccag
     1381 cagagcagtc agccctacgg acagcagagt tacagtggtt atagccagtc cacggacact
     1441 tcaggctatg gccagagcag ctattcttct tatggccaga gccagaacac aggctatgga
     1501 actcagtcaa ctccccaggg atatggctcg actggcggct atggcagtag ccagagctcc
     1561 caatcgtctt acgggcagca gtcctcctat cctggctatg gccagcagcc agctcccagc
     1621 agcacctcgg gaagttacgg tagcagttct cagagcagca gctatgggca gccccagagt
     1681 gggagctaca gccagcagcc tagctatggt ggacagcagc aaagctatgg acagcagcaa
     1741 agctataatc cccctcaggg ctatggacag cagaaccagt acaacagcag cagtggtggt
     1801 ggaggtggag gtggaggtgg aggtaactat ggccaagatc aatcctccat gagtagtggt
     1861 ggtggcagtg gtggcggtta tggcaatcaa gaccagagtg gtggaggtgg cagcggtggc
     1921 tatggacagc aggaccgtgg aggtggagga atggtgagca agggcgagga gctgttcacc
     1981 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     2041 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     2101 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgatg
     2161 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     2221 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     2281 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     2341 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     2401 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     2461 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     2521 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa
     2581 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2641 actctcggca tggacgagct gtacaaggcg gccacgcgta tgagcaattc agttttgtcc
     2701 acttcttccc caggtaatat ccaaactcgg caggcgattc cggcccaact cctctattcc
     2761 ctcgtcaacc aaaaagccag cggcaaactt gtggtacaaa acccctttga cgaatttgtt
     2821 tattggcaag tttatttggg ggatggtcgt attcacttgg ccaacagcgc caatggttgt
     2881 tccgaaagac ttaattatct gattggtagc acccttcgtc agaagggcat tgaactgcca
     2941 tcaagaattg ctgacgatta tatttttctt tccgggctct ggaaaaggga tgttttttcc
     3001 tttcagcaaa cccgctcgat tctgacccaa gctagccaag aagccttagt tcaggtactt
     3061 tccttaccta aaacatccta tcgttttaat caagaaaaag aactgagcga actatttttg
     3121 aatttagagt tagaaaaaac tattaccccc atcaaacata aaattcgcta ttggtgggaa
     3181 ttgaggtcct atattaactc gcctttccag cgacccctag tggaaaactg gcttagtctg
     3241 cgccaaaatt tggtgaaaaa taacctccat ggccaacatt ggctaaaacg cttccacact
     3301 tgcttggaaa atctaaattg cttatatgaa attgccgggc atttggaaat cagtactttg
     3361 gagttggcat taatgctaca gcccatggtc aagactggcg aaatcaagat gctgccttat
     3421 caagaaattc aaacggatga ccgccctttg gtggcttatg ttgaccatag ggaagtccag
     3481 cagcgcatga tgacctattc tttggaaaaa gaaggctatc gagccttagt actggatgat
     3541 ccctttcggg ctttaacggt gttgttgaat tctcccccca atctgattat cataaatact
     3601 gacttgccgg acatgaacgg ttatcaactc tgttcgtttt gccgtaagtc caatcaactt
     3661 aaagatgtgc cgatcctact attggggaaa ggtgacaatt tgatcagcgg tattcgtatc
     3721 aaactttgtc aagcttctgg gtacttaagt gagccttttg ttccagaaga attactcgat
     3781 cgggtggcaa aactccttcc caccaataaa accaactcct gagcggccgc aactcccacc
     3841 tgcaacatgc gtgactgact gaggccgcga ctctagagtc gacctgcagg catgcaagct
     3901 tgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     3961 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     4021 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     4081 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     4141 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     4201 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     4261 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     4321 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4381 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4441 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4501 ctccccgcat cgataccgtc gaccaaggca gctgtagatc ttagccactt tttaaaagaa
     4561 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     4621 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     4681 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     4741 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     4801 agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga
     4861 tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt
     4921 gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag
     4981 tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg
     5041 tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg
     5101 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     5161 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     5221 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
     5281 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
     5341 agggagagcg tcgaatggtg cactctcagt acaatctagc tctgatgccg catagttaag
     5401 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     5461 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     5521 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5581 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5641 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5701 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5761 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5821 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5881 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5941 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     6001 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     6061 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     6121 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6181 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     6241 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6301 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6361 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6421 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6481 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6541 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6601 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6661 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6721 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6781 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6841 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6901 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6961 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7021 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7081 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7141 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7201 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7261 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7321 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7381 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     7441 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     7501 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     7561 gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg
     7621 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7681 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7741 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     7801 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7861 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7921 ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc
     7981 actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact
     8041 ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt
     8101 ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg
     8161 ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg
     8221 gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa
     8281 ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc
     8341 gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac
     8401 ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt
     8461 gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc
     8521 ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc
     8581 tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg
     8641 cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct
     8701 gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg
     8761 ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca
     8821 ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat
     8881 cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata
     8941 cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg
     9001 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
     9061 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
     9121 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
     9181 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
     9241 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
     9301 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
     9361 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
     9421 aatattctgt aacctttata agtaggcata acagttataa tcataacata ctgttttttc
     9481 ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct
     9541 ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta
     9601 gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     9661 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     9721 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     9781 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     9841 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
     9901 accactgcca attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt
     9961 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc
    10021 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
    10081 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
    10141 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
    10201 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
    10261 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
    10321 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
    10381 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
    10441 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
    10501 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
    10561 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
    10621 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
    10681 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
    10741 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
    10801 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
    10861 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
    10921 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
    10981 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
    11041 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
    11101 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
    11161 gtgatcttca gacctggacg atatatatga gggacaattg gagaagtgaa ttatataaat
    11221 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
    11281 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
    11341 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
    11401 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
    11461 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctgg
//
