LOCUS       sequence-209506-       10788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For PixELL system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10788)
  AUTHORS   Dine E, Gil AA, Uribe G, Brangwynne CP, Toettcher JE
  TITLE     Protein Phase Separation Provides Long-Term Memory of Transient 
            Spatial Stimuli.
  JOURNAL   Cell Syst. 2018 May 24. pii: S2405-4712(18)30190-X. doi: 
            10.1016/j.cels.2018.05.002.
  PUBMED    29859829
REFERENCE   2  (bases 1 to 10788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 May 24. pii: S2405-4712(18)30190-X. doi: 
            10.1016/j.cels.2018.05.002."
COMMENT     Sequence Label: FUS-FusionRed-PixD
FEATURES             Location/Qualifiers
     source          1..10788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             253..297
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    580..697
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        851..1259
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1705..1716
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1976..2671
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     misc_feature    3234..3822
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3287..3307)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3705..3716)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3885..4118
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4143..4161)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4144..4161)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4451..4470)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4804..4826
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4864..4882)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4950..5054
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5055..5123,5124..5915)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5273..5292)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6086..6674
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6575..6594
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6828..6845
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6915..6935)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6920..7249
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7100..7235
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8383..8448
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8578..8598
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9023..9157
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9060..9079)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9114..9133
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9326..9959
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10006..10131
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(10623..10788,1..68)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
       61 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
      121 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
      181 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
      241 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
      301 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
      361 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
      421 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
      481 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
      541 ggatctcgac ggtcgccaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
      601 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
      661 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
      721 agatccagtt tggatcgata agcttgatat cgaattcctg cagccccgat aaaataaaag
      781 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gttatggcaa
      841 gctagctgca gtaacgccat tattgcaagg catggaaaaa taccaaacca agaatagaga
      901 agttcagatc aagggcgggt acatgaaaat agctaacgta gggccaaaca ggatatctgc
      961 ggtgagcagt ttcggccccg gcccggggcc aagaacagat ggtcaccgca gtttcggccc
     1021 cggcccgagg ccaagagcag atggtcccca gatatggccc aaccctcagc agtttcttaa
     1081 gacccatcag atgtttccag gctcccccaa ggacctgaaa tgaccctgcg ccttatttga
     1141 attaaccaat cagcctgctt ctcgcttctg ttcgcgcgct tctgcttccc gagctctata
     1201 aaagagctca caacccctca ctcggcgcgc cagtcctccg acagactgag tcgcccgggg
     1261 gggatctgga gctctcgaga attctcacgc gtcaagtgga gcaaggcagg tggacagtgg
     1321 atccatggcc tcaaacgatt atacccaaca agcaacccaa agctatgggg cctaccccac
     1381 ccagcccggg cagggctatt cccagcagag cagtcagccc tacggacagc agagttacag
     1441 tggttatagc cagtccacgg acacttcagg ctatggccag agcagctatt cttcttatgg
     1501 ccagagccag aacacaggct atggaactca gtcaactccc cagggatatg gctcgactgg
     1561 cggctatggc agtagccaga gctcccaatc gtcttacggg cagcagtcct cctatcctgg
     1621 ctatggccag cagccagctc ccagcagcac ctcgggaagt tacggtagca gttctcagag
     1681 cagcagctat gggcagcccc agagtgggag ctacagccag cagcctagct atggtggaca
     1741 gcagcaaagc tatggacagc agcaaagcta taatccccct cagggctatg gacagcagaa
     1801 ccagtacaac agcagcagtg gtggtggagg tggaggtgga ggtggaggta actatggcca
     1861 agatcaatcc tccatgagta gtggtggtgg cagtggtggc ggttatggca atcaagacca
     1921 gagtggtgga ggtggcagcg gtggctatgg acagcaggac cgtggaggtg gaggaatggt
     1981 gagcgagctg attaaggaga acatgcccat gaagctgtac atggagggca ccgtgaacaa
     2041 ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat
     2101 gagaatcaag gtcgtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctaccag
     2161 cttcatgtac ggcagcagaa ccttcatcaa gcaccctccg ggcatccccg acttctttaa
     2221 gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt
     2281 gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaaggt
     2341 tagaggggtg aacttcccag ccaacggccc tgtgatgcag aagaaaacac tcggctggga
     2401 ggcctccacc gagacgatgt accccgctga cggcggcctg gaaggcgcat gtgacatggc
     2461 cctgaagctc gtgggcgggg gccacctgat ctgcaacctt gagaccacat acagatccaa
     2521 gaaacccgct acgaacctca agatgcccgg cgtctacaac gtggaccaca gactggaaag
     2581 aatcaaggag gccgacgatg agacctacgt cgagcagcac gaggtggctg tggccagata
     2641 ctctactggt ggcgctggtg atggaggtaa agcggccacg cgtatgagtt tgtaccgttt
     2701 gatttacagc agtcagggca ttcccaattt acaaccccag gatttaaaag atatcttaga
     2761 atcttcccaa agaaataatc cggccaatgg cattaccgga ctgctctgct acagcaagcc
     2821 ggcttttctg caggtattgg aaggagagtg tgaacaggtt aatgaaacct accaccggat
     2881 tgtgcaagat gaacgccacc acagccccca gatcattgag tgtatgccga tcagaagaag
     2941 aaacttcgag gtttggtcta tgcaagcgat cacggtgaat gatttgtcaa cggaacaggt
     3001 taagactttg gtattgaaat attccggctt caccacccta cggcccagtg ccatggaccc
     3061 ggaacaatgc ctaaactttt tgctagacat tgccaagatt tacgagttgt ccgataactt
     3121 tttcctcgac ctctaagcgg ccgcaactcc cacctgcaac atgcgtgact gactgaggcc
     3181 gcgactctag agtcgacctg caggcatgca agcttgatat caagcttatc gataatcaac
     3241 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3301 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3361 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     3421 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     3481 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     3541 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     3601 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     3661 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     3721 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     3781 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgaccaa
     3841 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     3901 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     3961 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     4021 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     4081 cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt
     4141 attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag
     4201 ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag
     4261 accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga
     4321 gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc
     4381 gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     4441 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     4501 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     4561 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     4621 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct
     4681 cagtacaatc tagctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     4741 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     4801 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     4861 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     4921 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4981 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5041 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     5101 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     5161 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     5221 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     5281 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     5341 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     5401 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     5461 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     5521 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     5581 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     5641 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     5701 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     5761 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5821 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5881 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5941 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6001 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6061 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6121 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6181 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     6241 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6301 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6361 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6421 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6481 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6541 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6601 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6661 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6721 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6781 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     6841 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6901 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     6961 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     7021 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     7081 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7141 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     7201 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca
     7261 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca
     7321 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
     7381 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga
     7441 cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta
     7501 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg
     7561 gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt
     7621 ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga
     7681 aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt
     7741 tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg
     7801 tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc
     7861 gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct
     7921 ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta
     7981 ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct
     8041 ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg
     8101 tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga
     8161 tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga
     8221 tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg
     8281 gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg
     8341 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     8401 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     8461 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
     8521 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
     8581 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
     8641 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
     8701 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
     8761 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
     8821 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
     8881 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
     8941 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     9001 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     9061 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9121 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
     9181 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tagtggtttc
     9241 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
     9301 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
     9361 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     9421 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     9481 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     9541 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     9601 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     9661 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     9721 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     9781 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     9841 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     9901 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     9961 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
    10021 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
    10081 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
    10141 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
    10201 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
    10261 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
    10321 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
    10381 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
    10441 aacaaaagta agaccaccgc acagcaagcg gccggtgatc ttcagacctg gacgatatat
    10501 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
    10561 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
    10621 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
    10681 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
    10741 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtc
//
