LOCUS       sequence-209507-       11902 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For membrane localized optoDroplets.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11902)
  AUTHORS   Dine E, Gil AA, Uribe G, Brangwynne CP, Toettcher JE
  TITLE     Protein Phase Separation Provides Long-Term Memory of Transient 
            Spatial Stimuli.
  JOURNAL   Cell Syst. 2018 May 24. pii: S2405-4712(18)30190-X. doi: 
            10.1016/j.cels.2018.05.002.
  PUBMED    29859829
REFERENCE   2  (bases 1 to 11902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 May 24. pii: S2405-4712(18)30190-X. doi: 
            10.1016/j.cels.2018.05.002."
COMMENT     Sequence Label: Myristoylated optoDroplet
FEATURES             Location/Qualifiers
     source          1..11902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        312..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             754..774
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             1179..1190
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1450..2145
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     CDS             2164..3657
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    3809..4397
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3862..3882)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4280..4291)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4460..4693
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4718..4736)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4719..4736)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5026..5045)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     5379..5401
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5439..5457)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5525..5629
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5630..5698,5699..6490)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5848..5867)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6661..7249
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7150..7169
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7403..7420
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7490..7510)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7495..7824
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7675..7810
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8958..9023
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9153..9173
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9598..9732
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9635..9654)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9689..9708
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9901..10534
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10581..10706
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11198..11431
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11616..11660
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
      241 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggttatggca
      301 agctagctgc agtaacgcca ttattgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt agggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagagca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg cgtatgggtt cctccaaatc caagcccaag
      781 gacgcatctc agcgacgtcg cgcctcaaac gattataccc aacaagcaac ccaaagctat
      841 ggggcctacc ccacccagcc cgggcagggc tattcccagc agagcagtca gccctacgga
      901 cagcagagtt acagtggtta tagccagtcc acggacactt caggctatgg ccagagcagc
      961 tattcttctt atggccagag ccagaacaca ggctatggaa ctcagtcaac tccccaggga
     1021 tatggctcga ctggcggcta tggcagtagc cagagctccc aatcgtctta cgggcagcag
     1081 tcctcctatc ctggctatgg ccagcagcca gctcccagca gcacctcggg aagttacggt
     1141 agcagttctc agagcagcag ctatgggcag ccccagagtg ggagctacag ccagcagcct
     1201 agctatggtg gacagcagca aagctatgga cagcagcaaa gctataatcc ccctcagggc
     1261 tatggacagc agaaccagta caacagcagc agtggtggtg gaggtggagg tggaggtgga
     1321 ggtaactatg gccaagatca atcctccatg agtagtggtg gtggcagtgg tggcggttat
     1381 ggcaatcaag accagagtgg tggaggtggc agcggtggct atggacagca ggaccgtgga
     1441 ggtggaggaa tggtgagcga gctgattaag gagaacatgc ccatgaagct gtacatggag
     1501 ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
     1561 ggcacccaga ccatgagaat caaggtcgtc gagggcggcc ctctcccctt cgccttcgac
     1621 atcctggcta ccagcttcat gtacggcagc agaaccttca tcaagcaccc tccgggcatc
     1681 cccgacttct ttaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
     1741 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     1801 tacaacgtca aggttagagg ggtgaacttc ccagccaacg gccctgtgat gcagaagaaa
     1861 acactcggct gggaggcctc caccgagacg atgtaccccg ctgacggcgg cctggaaggc
     1921 gcatgtgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa ccttgagacc
     1981 acatacagat ccaagaaacc cgctacgaac ctcaagatgc ccggcgtcta caacgtggac
     2041 cacagactgg aaagaatcaa ggaggccgac gatgagacct acgtcgagca gcacgaggtg
     2101 gctgtggcca gatactctac tggtggcgct ggtgatggag gtaaagcggc cacgcgtacg
     2161 cgtatgaaga tggacaaaaa gactatagtt tggtttagaa gagacctaag gattgaggat
     2221 aatcctgcat tagcagcagc tgctcacgaa ggatctgttt ttcctgtctt catttggtgt
     2281 cctgaagaag aaggacagtt ttatcctgga agagcttcaa gatggtggat gaaacaatca
     2341 cttgctcact tatctcaatc cttgaaggct cttggatctg acctcacttt aatcaaaacc
     2401 cacaacacga tttcagcgat cttggattgt atccgcgtta ccggtgctac aaaagtcgtc
     2461 tttaaccacc tctatgatcc tgtttcgtta gttcgggacc ataccgtaaa ggagaagctg
     2521 gtggaacgtg ggatctctgt gcaaagctac aatggagatc tattgtatga accgtgggag
     2581 atatactgcg aaaagggcaa accttttacg agtttcaatt cttactggaa gaaatgctta
     2641 gatatgtcga ttgaatccgt tatgcttcct cctccttggc ggttgatgcc aataactgca
     2701 gcggctgaag cgatttgggc gtgttcgatt gaagaactag ggctggagaa tgaggccgag
     2761 aaaccgagca atgcgttgtt aactagagct tggtctccag gatggagcaa tgctgataag
     2821 ttactaaatg agttcatcga gaagcagttg atagattatg caaagaacag caagaaagtt
     2881 gttgggaatt ctacttcact actttctccg tatctccatt tcggggaaat aagcgtcaga
     2941 cacgttttcc agtgtgcccg gatgaaacaa attatatggg caagagataa gaacagtgaa
     3001 ggagaagaaa gtgcagatct ttttcttagg ggaatcggtt taagagagta ttctcggtat
     3061 atatgtttca acttcccgtt tactcacgag caatcgttgt tgagtcatct tcggtttttc
     3121 ccttgggatg ctgatgttga taagttcaag gcctggagac aaggcaggac cggttatccg
     3181 ttggtggatg ccggaatgag agagctttgg gctaccggat ggatgcataa cagaataaga
     3241 gtgattgttt caagctttgc tgtgaagttt cttctccttc catggaaatg gggaatgaag
     3301 tatttctggg atacactttt ggatgctgat ttggaatgtg acatccttgg ctggcagtat
     3361 atctctggga gtatccccga tggccacgag cttgatcgct tggacaatcc cgcgttacaa
     3421 ggcgccaaat atgacccaga aggtgagtac ataaggcaat ggcttcccga gcttgcgaga
     3481 ttgccaactg aatggatcca tcatccatgg gacgctcctt taaccgtact caaagcttct
     3541 ggtgtggaac tcggaacaaa ctatgcgaaa cccattgtag acatcgacac agctcgtgag
     3601 ctactagcta aagctatttc aagaacccgt gaagcacaga tcatgatcgg agcagcagcc
     3661 cgggatccac cggtcgccac cggttcaggg agcggatccg gctcagggag tgcggccgca
     3721 actcccacct gcaacatgcg tgactgactg aggccgcgac tctagagtcg acctgcaggc
     3781 atgcaagctt gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     3841 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     3901 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     3961 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     4021 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     4081 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     4141 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     4201 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     4261 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     4321 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     4381 ttgggccgcc tccccgcatc gataccgtcg accaaggcag ctgtagatct tagccacttt
     4441 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     4501 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     4561 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     4621 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     4681 aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta
     4741 tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca
     4801 agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg
     4861 ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc
     4921 cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac
     4981 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
     5041 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
     5101 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
     5161 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg
     5221 agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctagct ctgatgccgc
     5281 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     5341 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     5401 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     5461 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     5521 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5581 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5641 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5701 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5761 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5821 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5881 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5941 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6001 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6061 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6121 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6181 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6241 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6301 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6361 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6421 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6481 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6541 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6601 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6661 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6721 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6781 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     6841 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6901 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6961 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7021 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7081 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7141 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7201 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7261 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7321 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7381 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     7441 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
     7501 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7561 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7621 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     7681 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7741 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7801 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
     7861 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     7921 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     7981 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     8041 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     8101 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
     8161 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     8221 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     8281 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     8341 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     8401 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
     8461 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
     8521 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
     8581 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
     8641 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac
     8701 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
     8761 acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga
     8821 tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc
     8881 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     8941 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     9001 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     9061 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     9121 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     9181 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     9241 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     9301 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
     9361 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
     9421 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
     9481 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     9541 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     9601 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     9661 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     9721 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     9781 taccctatta ccactgccaa ttacctagtg gtttcattta ctctaaacct gtgattcctc
     9841 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
     9901 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
     9961 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
    10021 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
    10081 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
    10141 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
    10201 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
    10261 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
    10321 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
    10381 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    10441 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
    10501 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
    10561 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
    10621 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
    10681 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
    10741 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
    10801 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
    10861 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
    10921 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
    10981 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
    11041 aagcggccgg tgatcttcag acctggacga tatatatgag ggacaattgg agaagtgaat
    11101 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
    11161 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
    11221 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
    11281 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
    11341 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
    11401 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
    11461 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
    11521 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
    11581 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
    11641 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
    11701 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
    11761 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
    11821 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
    11881 acagatccat tcgattagtg aa
//
