LOCUS       sequence-209509-       14693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with 6 cassettes .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14693)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 14693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: ColorfulCell
FEATURES             Location/Qualifiers
     source          1..14693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        86..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             join(720..722,723..725,726..1436)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    1455..1679
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        1745..2048
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2049..2252
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2298..2307
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2340..2342,2343..2345,2346..3056)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3063..3065,3066..3068,3069..3779)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3810..3812,3813..4526)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    4533..4757
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        4823..5126
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5127..5330
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      5376..5385
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5388..5408
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7512..7736
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        7802..8105
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8106..8309
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             8436..9113
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQA"
     CDS             8436..9113
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQA"
     polyA_signal    9120..9344
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        9410..9713
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9714..9917
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             9963..10895
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     polyA_signal    10914..11138
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        11204..11507
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11508..11711
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             11822..12529
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(11967..11985)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    12554..12778
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        12801..12905
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12906..12974,12975..13766)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13124..13143)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13937..14525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14426..14445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac tcgagtagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
      601 tgaaccgtca gatccgctag ggatctcagc tggtcgagat gaagtcgccg cccctcgtgc
      661 tggccgccct ggtggcctgc atcatcgtct tgggcttcaa ctactggatt gcggtcgaga
      721 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      781 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      841 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      901 tcgtgaccac cttcggctac ggcctgatgt gcttcgcccg ctaccccgac cacatgaagc
      961 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     1021 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     1081 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     1141 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     1201 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     1261 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     1321 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     1381 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagacag
     1441 atccatgagt cgagctgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     1501 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     1561 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     1621 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggt
     1681 cgagtagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     1741 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     1801 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     1861 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     1921 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     1981 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     2041 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     2101 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     2161 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     2221 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagg
     2281 gatctcagct ggtcgaggcc accatgggct gtatcaagag caagaggaag gacgtcgaga
     2341 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     2401 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     2461 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     2521 tcgtgaccac cctgacctgg ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc
     2581 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     2641 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     2701 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     2761 agctggagta caacgccatc agcgacaacg tctatatcac cgccgacaag cagaagaacg
     2821 gcatcaaggc caacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     2881 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     2941 acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     3001 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggtcg
     3061 agatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     3121 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     3181 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     3241 ccctcgtgac caccctgacc tggggcgtgc agtgcttcgc ccgctacccc gaccacatga
     3301 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     3361 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     3421 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     3481 acaagctgga gtacaacgcc atcagcgaca acgtctatat caccgccgac aagcagaaga
     3541 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     3601 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     3661 actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     3721 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     3781 tcgagagctt tgtgatctct ggggtcgagg tgagcaaggg cgaggagctg ttcaccgggg
     3841 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     3901 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     3961 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc gtgcagtgct
     4021 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     4081 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     4141 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     4201 aggaggacgg caacatcctg gggcacaagc tggagtacaa cgccatcagc gacaacgtct
     4261 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
     4321 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     4381 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc
     4441 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     4501 tcggcatgga cgagctgtac aagtaagtcg agctgtgcct tctagttgcc agccatctgt
     4561 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4621 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     4681 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     4741 tgcggtgggc tctatggtcg agtagttatt aatagtaatc aattacgggg tcattagttc
     4801 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     4861 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     4921 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     4981 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     5041 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     5101 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     5161 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     5221 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     5281 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga
     5341 accgtcagat ccgctaggga tctcagctgg tcgaggccac catggctcct aagaagaaga
     5401 ggaaggtggt cgagagcgag ctgattaagg agaacatgca catgaagctg tacatggagg
     5461 gcaccgtgga caaccatcac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg
     5521 gcacccagac catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca
     5581 tcctggctac tagcttcctc tacggcagca agaccttcat caaccacacc cagggcatcc
     5641 ccgacttctt caagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg
     5701 aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct
     5761 acaacgtcaa gatcagaggg gtgaacttca catccaacgg ccctgtgatg cagaagaaaa
     5821 cactcggctg ggaggccttc accgagacgc tgtaccccgc tgacggcggc ctggaaggca
     5881 gaaacgacat ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac atcaagacca
     5941 catatagatc caagaaaccc gctaagaacc tcaagatgcc tggcgtctac tatgtggact
     6001 acagactgga aagaatcaag gaggccaaca acgagaccta cgtcgagcag cacgaggtgg
     6061 cagtggccag atacgcgggc ctgggcggcg gcctgaacgt cgagagcgag ctgattaagg
     6121 agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac ttcaagtgca
     6181 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg
     6241 agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc tacggcagca
     6301 agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc ttccctgagg
     6361 gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg
     6421 acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttca
     6481 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc accgagacgc
     6541 tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg
     6601 ggagccatct gatcgcaaac atcaagacca catatagatc caagaaaccc gctaagaacc
     6661 tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag gaggccaaca
     6721 acgagaccta cgtcgagcag cacgaggtgg cagtggccag atacgcgggc ctgggcggcg
     6781 gcctgaacgt cgaggtgagc tctatctttg gggtcgagag cgagctgatt aaggagaaca
     6841 tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg
     6901 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
     6961 gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct
     7021 tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca
     7081 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
     7141 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca
     7201 acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag acgctgtacc
     7261 ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc
     7321 atctgatcgc aaacatcaag accacatata gatccaagaa acccgctaag aacctcaaga
     7381 tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga
     7441 cctacgtcga gcagcacgag gtggcagtgg ccagatacgc gggcctgggc ggcggcctga
     7501 actaagtcga gctgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     7561 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     7621 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     7681 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggtcga
     7741 gtagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     7801 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     7861 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     7921 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     7981 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     8041 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     8101 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     8161 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     8221 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     8281 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagggat
     8341 ctcagctggt cgagatgctg agcctgcggc agagcatccg gttcttcaag cccgccaccc
     8401 ggaccctgtg cagcagccgg tacctgctgg tcgagatggt gagcgtgatc aagcccgaga
     8461 tgaagatcaa gctgtgcatg aggggcaccg tgaacggcca caacttcgtg atcgagggcg
     8521 agggcaaggg caacccctac gagggcaccc agatcctgga cctgaacgtg accgagggcg
     8581 cccccctgcc cttcgcctac gacatcctga ccaccgtgtt ccagtacggc aacagggcct
     8641 tcaccaagta ccccgccgac atccaggact acttcaagca gaccttcccc gagggctacc
     8701 actgggagag gagcatgacc tacgaggacc agggcatctg caccgccacc agcaacatca
     8761 gcatgagggg cgactgcttc ttctacgaca tcaggttcga cggcaccaac ttccccccca
     8821 acggccccgt gatgcagaag aagaccctga agtgggagcc cagcaccgag aagatgtacg
     8881 tggaggacgg cgtgctgaag ggcgacgtga acatgaggct gctgctggag ggcggcggcc
     8941 actacaggtg cgacttcaag accacctaca aggccaagaa ggaggtgagg ctgcccgacg
     9001 cccacaagat cgaccacagg atcgagatcc tgaagcacga caaggactac aacaaggtga
     9061 agctgtacga gaacgccgtg gccaggtact ccatgctgcc cagccaggcc taagtcgagc
     9121 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     9181 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     9241 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     9301 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggtcgagt agttattaat
     9361 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     9421 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     9481 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     9541 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     9601 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     9661 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     9721 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     9781 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     9841 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     9901 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagggatct cagctggtcg
     9961 agatggcgcg taaggtcgat ctcacctcct gcgatcgcga gccgatccac atccccggca
    10021 gcattcagcc gtgcggctgc ctgctagcct gcgacgcgca ggcggtgcgg atcacgcgca
    10081 ttacggaaaa tgccggcgcg ttctttggac gcgaaactcc gcgggtcggt gagctactcg
    10141 ccgattactt cggcgagacc gaagcccatg cgctgcgcaa cgcactggcg cagtcctccg
    10201 atccaaagcg accggcgctg atcttcggtt ggcgcgacgg cctgaccggc cgcaccttcg
    10261 acatctcact gcatcgccat gacggtacat cgatcatcga gttcgagcct gcggcggccg
    10321 aacaggccga caatccgctg cggctgacgc ggcagatcat cgcgcgcacc aaagaactga
    10381 agtcgctcga agagatggcc gcacgggtgc cgcgctatct gcaggcgatg ctcggctatc
    10441 accgcgtgat gttgtaccgc ttcgcggacg acggctccgg gatggtgatc ggcgaggcga
    10501 agcgcagcga cctggagagc tttctcggtc agcactttcc ggcgtcgctg gtcccgcagc
    10561 aggcgcggct actgtacttg aagaacgcga tccgcgtggt ctcggattcg cgcggcatca
    10621 gcagccggat cgtgcccgag cacgacgcct ccggcgccgc gctcgatctg tcgttcgcgc
    10681 acctgcgcag catctcgccc tgccatctcg aatttctgcg gaacatgggc gtcagcgcct
    10741 cgatgtcgct gtcgatcatc attgacggca cgctatgggg attgatcatc tgtcatcatt
    10801 acgagccgcg tgccgtgccg atggcgcagc gcgtcgcggc cgaaatgttc gccgacttct
    10861 tatcgctgca cttcaccgcc gcccaccacc aacgcagcaa gctgtgagtc gagctgtgcc
    10921 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
    10981 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
    11041 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
    11101 caatagcagg catgctgggg atgcggtggg ctctatggtc gagtagttat taatagtaat
    11161 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
    11221 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
    11281 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
    11341 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
    11401 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
    11461 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
    11521 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
    11581 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
    11641 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
    11701 taagcagagc tggtttagtg aaccgtcaga tccgctaggg atctcagctg gtcgagtcgc
    11761 caccatgctg ctatccgtgc cgttgctgct cggcctcctc ggcctggccg tcgccgtcga
    11821 gatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
    11881 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
    11941 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
    12001 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
    12061 ccccgccgac attcccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
    12121 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
    12181 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
    12241 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
    12301 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
    12361 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
    12421 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
    12481 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt ctaaggacga
    12541 gctgtgagtc gagctgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
    12601 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
    12661 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
    12721 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggtc
    12781 gaccaattcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    12841 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    12901 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    12961 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    13021 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    13081 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    13141 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    13201 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    13261 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    13321 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    13381 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    13441 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    13501 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    13561 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    13621 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    13681 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    13741 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    13801 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    13861 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    13921 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    13981 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    14041 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    14101 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    14161 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    14221 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    14281 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    14341 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    14401 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    14461 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    14521 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    14581 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    14641 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agc
//
