LOCUS       sequence-20951-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG306GPD-ccdB-DsRed
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(51..175)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(183..199)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(183..199)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(205..468)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(288..311)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 cgaagccgga agcataaagt gtaaagcctg ggggtgccta atgcggccgc catagtgact
       61 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
      121 tattgatatt tatatcattt tacgtttctc gttcagcttt tttgtacaaa cttgtgatgg
      181 gggatccact agttctagaa tccgtcgaaa ctaagttctg gtgttttaaa actaaaaaaa
      241 agactaacta taaaagtaga atttaagaag tttaagaaat agatttacag aattacaatc
      301 aatacctacc gtctttatat acttattagt caagtagggg aataatttca gggaactggt
      361 ttcaaccttt tttttcagct ttttccaaat cagagagagc agaaggtaat agaaggtgta
      421 agaaaatgag atagatacat gcgtgggtca attgccttgt gtcatcattt actccaggca
      481 ggttgcatca ctccattgag gttgtgcccg ttttttgcct gtttgtgccc ctgttctctg
      541 tagttgcgct aagagaatgg acctatgaac tgatggttgg tgaagaaaac aatattttgg
      601 tgctgggatt cttttttttt ctggatgcca gcttaaaaag cgggctccat tatatttagt
      661 ggatgccagg aataaactgt tcacccagac acctacgatg ttatatattc tgtgtaaccc
      721 gccccctatt ttgggcatgt acgggttaca gcagaa
//
