LOCUS       sequence-209562-        9961 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a plasma membrane calcium pump hPMCA2w/b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9961)
  AUTHORS   Yu X, Taylor AMW, Nagai J, Golshani P, Evans CJ, Coppola G, Khakh BS
  TITLE     Reducing Astrocyte Calcium Signaling In Vivo Alters Striatal 
            Microcircuits and Causes Repetitive Behavior.
  JOURNAL   Neuron. 2018 Aug 27. pii: S0896-6273(18)30688-3. doi: 
            10.1016/j.neuron.2018.08.015.
  PUBMED    30174118
REFERENCE   2  (bases 1 to 9961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2018 Aug 27. pii: S0896-6273(18)30688-3. doi: 
            10.1016/j.neuron.2018.08.015."
COMMENT     Sequence Label: pcDNA3.1-CMV-mCherry-hPMCA2w/b
FEATURES             Location/Qualifiers
     source          1..9961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        925..943
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             949..1656
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1094..1112)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1362..1381
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1672..1698
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1699..1716
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1717..1734
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      1786..1795
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2293..2304)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      2854..2863
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5555..5572)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5561..5785
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5831..6259
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5918..5937)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6128..6149
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6268..6288)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6273..6602
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6453..6588
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6515..6534
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6669..7463
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6723..6742)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7333..7352
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7637..7758
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7674..7693)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7728..7747
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7807..7823)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7807..7823)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7820..7842)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7831..7847
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7855..7861,7862..7879,7880..7885))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7900..7921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8038..8055)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8209..8794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8289..8308)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8965..9756,9757..9825))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9588..9607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9826..9930)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aaggctagag tacttaatac gactcactat aggctagcat ggtgagcaag
      961 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     1021 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1081 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     1141 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     1201 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     1261 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     1321 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     1381 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     1441 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     1501 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     1561 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     1621 cgccactcca ccggcggcat ggacgagctg tacaagtccg gactcagatc ctacccctat
     1681 gatgtgcctg actatgccca ccaccaccac caccacctgg tgcccagggg cagcagatct
     1741 cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg tggcggccgc catgggtgac
     1801 atgaccaaca gcgactttta ctccaaaaac caaagaaatg agtcgagcca tgggggcgag
     1861 ttcgggtgca caatggagga gctccgctcc ctcatggagc tgcggggcac tgaggctgtg
     1921 gtcaagatca aggagactta tggggacacc gaagccatct gccggcgcct caaaacctca
     1981 cctgttgaag gtttgccgga caccgctcca gacctggaaa agagaaagca aatttttggg
     2041 caaaacttta tacctccaaa gaagccaaaa accttcctgc agctcgtgtg ggaggcgctg
     2101 caggacgtga cgctcatcat cctggagatt gccgccatca tctccctggg gctgtccttc
     2161 taccacccgc ccggcgaggg caacgaagga tgtgcgacgg cccagggtgg ggcagaggat
     2221 gaaggagagg cagaggcagg ttggatcgag ggggccgcca ttctcctctc agttatctgt
     2281 gtggtcctgg tcacggcctt caatgactgg agcaaagaga aacagttccg gggcctgcag
     2341 agccgcatcg agcaggaaca gaaatttacc gtagtccggg ctggccaggt ggtgcagatc
     2401 cctgtggctg agatcgtggt tggggacata gcccaggtca aatatggtga cctcctccct
     2461 gccgacggcc tcttcatcca gggcaatgac ctcaagattg atgaaagctc cctaactgga
     2521 gagtctgacc aggtgcgcaa gtccgtggac aaggacccca tgctgctgtc aggaacccac
     2581 gtgatggagg gctcaggacg gatgttggtg actgctgtgg gtgtgaactc tcagactggc
     2641 atcatcttta ccctcctggg ggctggtggt gaagaggaag agaagaaaga caaaaaaggt
     2701 gtgaagaagg gggatggcct tcagctacca gcagcagacg gtgcggcagc ttcaaatgct
     2761 gcagatagtg cgaatgccag cctagtcaat ggtaaaatgc aggatggcaa tgtggacgcc
     2821 agccagagca aagccaaaca gcaggacggg gcagccgcca tggagatgca gcccctcaag
     2881 agtgccgagg gcggcgacgc tgacgacagg aagaaggcca gcatgcacaa gaaggagaag
     2941 tccgtgctgc agggcaagct caccaagctg gctgtgcaga tcgggaaggc gggcttggtg
     3001 atgtcagcca tcacggtgat catcctggtg ctctacttca ctgtggacac cttcgtggtc
     3061 aacaagaagc cgtggctgcc tgagtgcacg cccgtctacg tgcagtactt tgtcaagttc
     3121 ttcatcattg gcgtgacggt gctggtggtc gccgtgcccg aggggctccc tctggccgtc
     3181 accatctcgt tggcctattc ggtgaagaaa atgatgaagg acaacaacct ggtacgccac
     3241 ctggatgcct gtgagaccat gggcaatgcc acagccatct gctcagacaa gacaggcacg
     3301 ctgaccacca atcgcatgac agtggtacag gcctatgtcg gcgacgtcca ctataaagag
     3361 atccccgacc ccagctccat caacaccaag accatggagc tgctgatcaa tgccatcgcc
     3421 atcaacagcg cctacaccac caagattctg cccccagaga aggagggcgc cctgcctcgg
     3481 caggtgggca acaagacgga gtgcggcctg ctgggcttcg tgctggacct gaagcaggac
     3541 tacgagcccg tgcgcagcca gatgccagag gagaagttgt acaaagtgta caccttcaac
     3601 tccgtgcgca agtccatgag cactgtcatc aagctgcccg acgagagctt ccgcatgtac
     3661 agcaaggggg cttctgagat cgtgctcaag aagtgctgca aaatcctcaa tggggcggga
     3721 gagcctcgtg tcttccggcc ccgcgaccgg gacgagatgg taaagaaggt gattgagccc
     3781 atggcttgcg atgggctccg cactatctgc gtggcctacc gcgacttccc cagcagcccg
     3841 gagccggact gggacaatga gaatgacatc ctcaacgaac tcacctgcat ctgcgtggtg
     3901 ggcatcgagg acccggtgcg gccagaggtc ccagaagcca tccgcaagtg ccagcgggca
     3961 ggcatcacgg tccgcatggt cactggcgac aatatcaaca cggctcgggc catcgccatc
     4021 aagtgtggca tcatccatcc tggggaggac tttctgtgcc tcgagggcaa ggagttcaac
     4081 aggaggatcc gcaacgagaa gggggagatt gagcaggagc gaattgacaa gatctggcca
     4141 aagctgcggg tgctggctcg ctcctcccca acggacaagc ataccctggt taaaggcatc
     4201 atcgacagca cacacactga gcagcggcag gtggtggccg tgacggggga cgggaccaac
     4261 gacgggcctg cactcaagaa ggccgacgtg ggcttcgcca tgggcatcgc aggcactgac
     4321 gtggccaagg aggcctcaga catcatcctg acagacgaca atttcagcag catcgtcaag
     4381 gcagtgatgt ggggccgcaa cgtctatgac agcatctcca aattcttgca gttccagctc
     4441 accgtcaacg tggtggccgt gattgtggcc ttcacaggcg cctgcatcac gcaggactcc
     4501 cctctgaagg ccgtgcagat gctctgggtg aacctcatca tggacacgtt tgcctcgctg
     4561 gcactggcca ctgagccgcc cacggagacc ctgctgctga ggaagccgta cggccgcaac
     4621 aagccgctca tctccaggac catgatgaag aacatcctgg gccatgctgt ctaccagctt
     4681 gccctcatct tcaccctgct ctttgttggc gagaagatgt tccagatcga cagcgggagg
     4741 aacgcgcccc tgcattcgcc accctcagaa cattacacca tcatcttcaa caccttcgtc
     4801 atgatgcagc tcttcaacga gatcaacgcc cgcaagatcc acggcgagcg caatgtcttt
     4861 gacggcatct tccggaaccc catcttctgc accatcgtgc tgggcacctt tgccatccag
     4921 atagtgatcg tgcagtttgg agggaagcca ttcagctgct ctccactgca gctggaccag
     4981 tggatgtggt gcatattcat tgggttagga gagctcgttt ggggccaggt catcgccacc
     5041 atcccgacca gcagactcaa gttcctcaag gaggcaggca ggctcacaca gaaggaggag
     5101 atcccggagg aggagctcaa cgaggacgtg gaggagatcg accacgcgga gcgggagctg
     5161 cggcggggcc agatcctgtg gttccgaggc ctgaatcgga tccagacaca gatccgcgtc
     5221 gtgaaggcgt tccgtagctc tctctatgaa ggtttagaaa agcctgaatc tcgaacctcc
     5281 atccataact tcatggctca tcctgaattc cggatcgaag attcccagcc ccacatcccc
     5341 ctcattgatg acaccgacct ggaagaagat gccgcgctca agcagaactc gagcccgccg
     5401 tcatccctca acaagaacaa cagcgccatc gacagtggga tcaacctgac gaccgacaca
     5461 agcaaatcag ctacctcttc aagtccaggg agccccatcc acagcctgga gacgtcgctt
     5521 tagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     5581 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     5641 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     5701 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     5761 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     5821 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5881 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5941 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6001 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6061 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6121 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6181 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6241 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     6301 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     6361 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6421 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     6481 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     6541 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     6601 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     6661 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     6721 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     6781 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     6841 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     6901 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     6961 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     7021 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     7081 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     7141 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     7201 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     7261 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     7321 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     7381 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     7441 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     7501 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     7561 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     7621 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7681 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7741 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     7801 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7861 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7921 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7981 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8041 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8101 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8161 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8221 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8281 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8341 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8401 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8461 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8521 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8581 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8641 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8701 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa
     8761 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8821 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8881 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8941 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9001 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9061 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9121 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9181 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9241 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9301 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9361 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9421 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9481 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9541 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9601 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9661 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9721 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9781 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9841 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9901 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9961 c
//
