LOCUS       sequence-209563-        9868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a plasma membrane calcium pump hPMCA2x/b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Yu X, Taylor AMW, Nagai J, Golshani P, Evans CJ, Coppola G, Khakh BS
  TITLE     Reducing Astrocyte Calcium Signaling In Vivo Alters Striatal 
            Microcircuits and Causes Repetitive Behavior.
  JOURNAL   Neuron. 2018 Aug 27. pii: S0896-6273(18)30688-3. doi: 
            10.1016/j.neuron.2018.08.015.
  PUBMED    30174118
REFERENCE   2  (bases 1 to 9868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2018 Aug 27. pii: S0896-6273(18)30688-3. doi: 
            10.1016/j.neuron.2018.08.015."
COMMENT     Sequence Label: pcDNA3.1-CMV-mCherry-hPMCA2x/b
FEATURES             Location/Qualifiers
     source          1..9868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        925..943
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             949..1656
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1094..1112)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1362..1381
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1672..1698
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1699..1716
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1717..1734
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      1786..1795
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2293..2304)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      2761..2770
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5462..5479)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5468..5692
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5738..6166
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5825..5844)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6035..6056
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6175..6195)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6180..6509
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6360..6495
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6422..6441
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6576..7370
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6630..6649)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7240..7259
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7544..7665
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7581..7600)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7635..7654
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7714..7730)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7714..7730)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7727..7749)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7738..7754
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7762..7768,7769..7786,7787..7792))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7807..7828
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7945..7962)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8116..8701)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8196..8215)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8872..9663,9664..9732))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9495..9514
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9733..9837)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aaggctagag tacttaatac gactcactat aggctagcat ggtgagcaag
      961 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     1021 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1081 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     1141 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     1201 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     1261 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     1321 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     1381 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     1441 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     1501 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     1561 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     1621 cgccactcca ccggcggcat ggacgagctg tacaagtccg gactcagatc ctacccctat
     1681 gatgtgcctg actatgccca ccaccaccac caccacctgg tgcccagggg cagcagatct
     1741 cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg tggcggccgc catgggtgac
     1801 atgaccaaca gcgactttta ctccaaaaac caaagaaatg agtcgagcca tgggggcgag
     1861 ttcgggtgca caatggagga gctccgctcc ctcatggagc tgcggggcac tgaggctgtg
     1921 gtcaagatca aggagactta tggggacacc gaagccatct gccggcgcct caaaacctca
     1981 cctgttgaag gtttgccgga caccgctcca gacctggaaa agagaaagca aatttttggg
     2041 caaaacttta tacctccaaa gaagccaaaa accttcctgc agctcgtgtg ggaggcgctg
     2101 caggacgtga cgctcatcat cctggagatt gccgccatca tctccctggg gctgtccttc
     2161 taccacccgc ccggcgaggg caacgaagga tgtgcgacgg cccagggtgg ggcagaggat
     2221 gaaggagagg cagaggcagg ttggatcgag ggggccgcca ttctcctctc agttatctgt
     2281 gtggtcctgg tcacggcctt caatgactgg agcaaagaga aacagttccg gggcctgcag
     2341 agccgcatcg agcaggaaca gaaatttacc gtagtccggg ctggccaggt ggtgcagatc
     2401 cctgtggctg agatcgtggt tggggacata gcccaggtca aatatggtga cctcctccct
     2461 gccgacggcc tcttcatcca gggcaatgac ctcaagattg atgaaagctc cctaactgga
     2521 gagtctgacc aggtgcgcaa gtccgtggac aaggacccca tgctgctgtc aggaacccac
     2581 gtgatggagg gctcaggacg gatgttggtg actgctgtgg gtgtgaactc tcagactggc
     2641 atcatcttta ccctcctggg ggctggtggt gaagaggaag agaagaaaga caaaaaaggt
     2701 aaaatgcagg atggcaatgt ggacgccagc cagagcaaag ccaaacaaca ggacggggca
     2761 gccgccatgg agatgcagcc cctcaagagt gccgagggcg gcgacgctga cgacaggaag
     2821 aaggccagca tgcacaagaa ggagaagtcc gtgctgcagg gcaagctcac caagctggct
     2881 gtgcagatcg ggaaggcggg cttggtgatg tcagccatca cggtgatcat cctggtgctc
     2941 tacttcactg tggacacctt cgtggtcaac aagaagccgt ggctgcctga gtgcacgccc
     3001 gtctacgtgc agtactttgt caagttcttc atcattggcg tgacggtgct ggtggtcgcc
     3061 gtgcccgagg ggctccctct ggccgtcacc atctcgttgg cctattcggt gaagaaaatg
     3121 atgaaggaca acaacctggt acgccacctg gatgcctgtg agaccatggg caatgccaca
     3181 gccatctgct cagacaagac aggcacgctg accaccaatc gcatgacagt ggtacaggcc
     3241 tatgtcggcg acgtccacta taaagagatc cccgacccca gctccatcaa caccaagacc
     3301 atggagctgc tgatcaatgc catcgccatc aacagcgcct acaccaccaa gattctgccc
     3361 ccagagaagg agggcgccct gcctcggcag gtgggcaaca agacggagtg cggcctgctg
     3421 ggcttcgtgc tggacctgaa gcaggactac gagcccgtgc gcagccagat gccagaggag
     3481 aagttgtaca aagtgtacac cttcaactcc gtgcgcaagt ccatgagcac tgtcatcaag
     3541 ctgcccgacg agagcttccg catgtacagc aagggggctt ctgagatcgt gctcaagaag
     3601 tgctgcaaaa tcctcaatgg ggcgggagag cctcgtgtct tccggccccg cgaccgggac
     3661 gagatggtaa agaaggtgat tgagcccatg gcttgcgatg ggctccgcac tatctgcgtg
     3721 gcctaccgcg acttccccag cagcccggag ccggactggg acaatgagaa tgacatcctc
     3781 aacgaactca cctgcatctg cgtggtgggc atcgaggacc cggtgcggcc agaggtccca
     3841 gaagccatcc gcaagtgcca gcgggcaggc atcacggtcc gcatggtcac tggcgacaat
     3901 atcaacacgg ctcgggccat cgccatcaag tgtggcatca tccatcctgg ggaggacttt
     3961 ctgtgcctcg agggcaagga gttcaacagg aggatccgca acgagaaggg ggagattgag
     4021 caggagcgaa ttgacaagat ctggccaaag ctgcgggtgc tggctcgctc ctccccaacg
     4081 gacaagcata ccctggttaa aggcatcatc gacagcacac acactgagca gcggcaggtg
     4141 gtggccgtga cgggggacgg gaccaacgac gggcctgcac tcaagaaggc cgacgtgggc
     4201 ttcgccatgg gcatcgcagg cactgacgtg gccaaggagg cctcagacat catcctgaca
     4261 gacgacaatt tcagcagcat cgtcaaggca gtgatgtggg gccgcaacgt ctatgacagc
     4321 atctccaaat tcttgcagtt ccagctcacc gtcaacgtgg tggccgtgat tgtggccttc
     4381 acaggcgcct gcatcacgca ggactcccct ctgaaggccg tgcagatgct ctgggtgaac
     4441 ctcatcatgg acacgtttgc ctcgctggca ctggccactg agccgcccac ggagaccctg
     4501 ctgctgagga agccgtacgg ccgcaacaag ccgctcatct ccaggaccat gatgaagaac
     4561 atcctgggcc atgctgtcta ccagcttgcc ctcatcttca ccctgctctt tgttggcgag
     4621 aagatgttcc agatcgacag cgggaggaac gcgcccctgc attcgccacc ctcagaacat
     4681 tacaccatca tcttcaacac cttcgtcatg atgcagctct tcaacgagat caacgcccgc
     4741 aagatccacg gcgagcgcaa tgtctttgac ggcatcttcc ggaaccccat cttctgcacc
     4801 atcgtgctgg gcacctttgc catccagata gtgatcgtgc agtttggagg gaagccattc
     4861 agctgctctc cactgcagct ggaccagtgg atgtggtgca tattcattgg gttaggagag
     4921 ctcgtttggg gccaggtcat cgccaccatc ccgaccagca gactcaagtt cctcaaggag
     4981 gcaggcaggc tcacacagaa ggaggagatc ccggaggagg agctcaacga ggacgtggag
     5041 gagatcgacc acgcggagcg ggagctgcgg cggggccaga tcctgtggtt ccgaggcctg
     5101 aatcggatcc agacacagat ccgcgtcgtg aaggcgttcc gtagctctct ctatgaaggt
     5161 ttagaaaagc ctgaatctcg aacctccatc cataacttca tggctcatcc tgaattccgg
     5221 atcgaagatt cccagcccca catccccctc attgatgaca ccgacctgga agaagatgcc
     5281 gcgctcaagc agaactcgag cccgccgtca tccctcaaca agaacaacag cgccatcgac
     5341 agtgggatca acctgacgac cgacacaagc aaatcagcta cctcttcaag tccagggagc
     5401 cccatccaca gcctggagac gtcgctttag tctagagggc ccgtttaaac ccgctgatca
     5461 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     5521 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     5581 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     5641 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     5701 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     5761 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5821 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5881 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5941 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6001 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6061 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6121 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     6181 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6241 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6301 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     6361 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     6421 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     6481 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     6541 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     6601 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     6661 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     6721 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     6781 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     6841 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     6901 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     6961 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     7021 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     7081 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     7141 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     7201 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     7261 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     7321 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     7381 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     7441 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     7501 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     7561 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7621 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     7681 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7741 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7801 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     7861 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7921 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7981 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8041 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8101 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8161 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8221 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8281 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8341 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8401 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8461 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8521 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     8581 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8641 cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8701 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8761 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8821 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8881 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8941 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9001 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9061 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9121 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9181 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9241 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9301 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9361 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9421 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9481 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9541 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9601 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9661 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9721 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9781 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9841 atttccccga aaagtgccac ctgacgtc
//
