LOCUS       sequence-209564-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ARH3 (amino acids 14-363) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Liszczak G, Diehl KL, Dann GP, Muir TW
  TITLE     Acetylation blocks DNA damage-induced chromatin ADP-ribosylation.
  JOURNAL   Nat Chem Biol. 2018 Jul 16. pii: 10.1038/s41589-018-0097-1. doi: 
            10.1038/s41589-018-0097-1.
  PUBMED    30013063
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2018 Jul 16. pii: 10.1038/s41589-018-0097-1. doi: 
            10.1038/s41589-018-0097-1."
COMMENT     Sequence Label: pET30-ARH3-His-Sumo-HA
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..69
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             76..369
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 ggagatatac atatgggcag cagccatcnt natnntcatc acggcagcgg cctggtgccg
       61 cgcggcagcg ctagcatgtc ggactcagaa gtcaatcaag aagctaagcc agaggtcaag
      121 ccagaagtca agcctgagac tcacatcaat ttaaaggtgt ccgatggatc ttcagagatc
      181 ttcttcaaga tcaaaaagac cactccttta agaaggctga tggaagcgtt cgctaaaaga
      241 cagggtaagg aaatggactc cttaagattc ttgtacgacg gtattagaat tcaagctgat
      301 cagacccctg aagatttgga catggaggat aacgatatta ttgaggctca cagagaacag
      361 attggtggtg ctggagcggc acgtagcctg agccgcttcc gtggctgctt ggctggtgct
      421 ctgttaggag attgcgtagg gtccttttac gaagctcatg acaccgtcga tttaacgagc
      481 gtacttcgtc acgttcaaag cctggaacct gatcccggga cgcctggttc tgagcgtact
      541 gaagcattat attacaccga cgatactgcc atggcccgcg cccttgttca gtctctttta
      601 gctaaggagg cgttcgacga ggtggacatg gctcaccgtt tcgcgcagga atacaagaaa
      661 gaccccgacc gtggatacgg tgcaggtgta gtgaccgtat ttaagaaact tttaaacccg
      721 aaatgtcgtg acgtttttga accagcccgt gctcaattca atggtaaagg ttcgtatggc
      781 aacggtgggg ctatg
//
