LOCUS       sequence-20958-e         677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG413GPD-ccdB-DsRed
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(42..166)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(174..190)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(174..190)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(196..459)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(279..302)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 aagcataaag tgtaaagcct gggggtgcct aatgcggccg ccatagtgac tggatatgtt
       61 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
      121 ttatatcatt ttacgtttct cgttcagctt ttttgtacaa acttgtgatg ggggatccac
      181 tagttctaga atccgtcgaa actaagttct ggtgttttaa aactaaaaaa aagactaact
      241 ataaaagtag aatttaagaa gtttaagaaa tagatttaca gaattacaat caatacctac
      301 cgtctttata tacttattag tcaagtaggg gaataatttc agggaactgg tttcaacctt
      361 ttttttcagc tttttccaaa tcagagagag cagaaggtaa tagaaggtgt aagaaaatga
      421 gatagataca tgcgtgggtc aattgccttg tgtcatcatt tactccaggc aggttgcatc
      481 actccattga ggttgtgccc gttttttgcc tgtttgtgcc cctgttctct gtagttgcgc
      541 taagagaatg gacctatgaa ctgatggttg gtgaagaaaa caatattttg gtgctgggat
      601 tctttttttt tttggatgcc agcttaaaaa gcgggctcca ttatatttag tggatgccag
      661 gaataaactg ttcaccc
//
