LOCUS       sequence-209589-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses full length PARP1 with K to Q mutations in 
            automodification domain in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Liszczak G, Diehl KL, Dann GP, Muir TW
  TITLE     Acetylation blocks DNA damage-induced chromatin ADP-ribosylation.
  JOURNAL   Nat Chem Biol. 2018 Jul 16. pii: 10.1038/s41589-018-0097-1. doi: 
            10.1038/s41589-018-0097-1.
  PUBMED    30013063
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2018 Jul 16. pii: 10.1038/s41589-018-0097-1. doi: 
            10.1038/s41589-018-0097-1."
COMMENT     Sequence Label: pCMV-PARP1-3xFlag-7xKQ
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             64..87
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             97..120
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             130..153
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gctaccggac tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccatgggt
       61 tcagactata aagatgatga tgataaaggt ggctccgact acaaggatga cgatgacaag
      121 ggaggtagtg actacaaaga cgatgacgac aagggcggat ctggcggatc tatggcggag
      181 tcttcggata agctctatcg agtcgagtac gccaagagcg ggcgcgcctc ttgcaagaaa
      241 tgcagcgaga gcatccccaa ggactcgctc cggatggcca tcatggtgca gtcgcccatg
      301 tttgatggaa aagtcccaca ctggtaccac ttctcctgct tctggaaggt gggccactcc
      361 atccggcacc ctgacgttga ggtggatggg ttctctgagc ttcggtggga tgatcagcag
      421 aaagtcaaga agacagcgga agctggagga gtgacaggca aaggccagga tggaattggt
      481 agcaaggcag agaagactct gggtgacttt gcagcagagt atgccaagtc caacagaagt
      541 acgtgcaagg ggtgtatgga gaagatagaa aagggccagg tgcgcctgtc caagaagatg
      601 gtggacccgg agaagccaca gctaggcatg attgaccgct ggtaccatcc aggctgcttt
      661 gtcaagaaca gggaggagct gggtttccgg cccgagtaca gtgcgagtca gctcaagggc
      721 ttcagcctcc ttgctacaga ggataaa
//
