LOCUS       sequence-209600-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses full length PARP1 with K to Q mutations in 
            automodification domain in Sf9 (insect) cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Liszczak G, Diehl KL, Dann GP, Muir TW
  TITLE     Acetylation blocks DNA damage-induced chromatin ADP-ribosylation.
  JOURNAL   Nat Chem Biol. 2018 Jul 16. pii: 10.1038/s41589-018-0097-1. doi: 
            10.1038/s41589-018-0097-1.
  PUBMED    30013063
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2018 Jul 16. pii: 10.1038/s41589-018-0097-1. doi: 
            10.1038/s41589-018-0097-1."
COMMENT     Sequence Label: pACEBac1-PARP1-Flag-His-7xKQ
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             583..606
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             646..663
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cacaatgcgt atgacttgga agtcatcgat atctttaaga tagagcgtga aggcgaatgc
       61 cagcgttaca agccctttaa gcagcttcat aaccgaagat tgctgtggca cgggtccagg
      121 accaccaact ttgctgggat cctgtcccag ggtcttcgga tagccccgcc tgaagcgccc
      181 gtgacaggct acatgtttgg taaagggatc tatttcgctg acatggtctc caagagtgcc
      241 aactactgcc atacgtctca gggagaccca ataggcttaa tcctgttggg agaagttgcc
      301 cttggaaaca tgtatgaact gaagcacgct tcacatatca gcaagttacc caagggcaag
      361 cacagtgtca aaggtttggg caaaactacc cctgatcctt cagctaacat tagtctggat
      421 ggtgtagacg ttcctcttgg gaccgggatt tcatctggtg tgaatgacac ctctctacta
      481 tataacgagt acattgtcta tgatattgct caggtaaatc tgaagtatct gctgaaactg
      541 aaattcaatt ttaagacctc cctgtggggc ggatctggcg gtgactataa agatgatgat
      601 gataaaggtg gtagtggagt cgacgagctc actggaggta gcggacacca tcaccatcac
      661 cactgatgtc gcggccgctt tcgaatctag agcctgcagt ctcgacaagc ttgtcga
//
