LOCUS       sequence-209603-         674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses full length PARP1 in Sf9 (insect) cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Liszczak G, Diehl KL, Dann GP, Muir TW
  TITLE     Acetylation blocks DNA damage-induced chromatin ADP-ribosylation.
  JOURNAL   Nat Chem Biol. 2018 Jul 16. pii: 10.1038/s41589-018-0097-1. doi: 
            10.1038/s41589-018-0097-1.
  PUBMED    30013063
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2018 Jul 16. pii: 10.1038/s41589-018-0097-1. doi: 
            10.1038/s41589-018-0097-1."
COMMENT     Sequence Label: pACEBac1-PARP1-Flag-His-WT
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             505..528
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             568..585
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aagcagcttc ataaccgaag attgctgtgg cacgggtcca ggaccaccaa ctttgctggg
       61 atcctgtccc agggtcttcg gatagccccg cctgaagcgc ccgtgacagg ctacatgttt
      121 ggtaaaggga tctatttcgc tgacatggtc tccaagagtg ccaactactg ccatacgtct
      181 cagggagacc caataggctt aatcctgttg ggagaagttg cccttggaaa catgtatgaa
      241 ctgaagcacg cttcacatat cagcaagtta cccaagggca agcacagtgt caaaggtttg
      301 ggcaaaacta cccctgatcc ttcagctaac attagtctgg atggtgtaga cgttcctctt
      361 gggaccggga tttcatctgg tgtgaatgac acctctctac tatataacga gtacattgtc
      421 tatgatattg ctcaggtaaa tctgaagtat ctgctgaaac tgaaattcaa ttttaagacc
      481 tccctgtggg gcggatctgg cggtgactat aaagatgatg atgataaagg tggtagtgga
      541 gtcgacgagc tcactggagg tagcggacac catcaccatc accactgatg tcgcggccgc
      601 tttcgaatct agagcctgca gtctcgacaa gcttgtcgag aagtactaga ggatcataat
      661 cagccatacc acat
//
