LOCUS       sequence-209613-        2785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Adeno-Associated viral (AAV) vector plasmid expressing mouse 
            Beclin-1 (BECN1) in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Pircs K, Petri R, Madsen S, Brattas PL, Vuono R, Ottosson DR, 
            St-Amour I, Hersbach BA, Matusiak-Bruckner M, Lundh SH, Petersen A, 
            Deglon N, Hebert SS, Parmar M, Barker RA, Jakobsson J
  TITLE     Huntingtin Aggregation Impairs Autophagy, Leading to Argonaute-2 
            Accumulation and Global MicroRNA Dysregulation.
  JOURNAL   Cell Rep. 2018 Aug 7;24(6):1397-1406. doi: 
            10.1016/j.celrep.2018.07.017.
  PUBMED    30089251
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.07"; journalName: "Cell Rep"; date: 
            "2018-08-7- 7"; volume: "24"; issue: "6"; pages: "1397-1406"
COMMENT     Sequence Label: pAAV2-SBECN1W
FEATURES             Location/Qualifiers
     source          1..2785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        109..556
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    1965..2553
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2018..2038)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2436..2447)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2601..2735
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2638..2657)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2692..2711
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctc agatcgggct
       61 gcaggaattg gccgctctag actctagctg cagagggacc tgcgtatgag tgcaagtggg
      121 ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc ccgacccact
      181 ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga gggggagggg
      241 aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga cagtgccttc
      301 gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc tgacgtcact
      361 cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg
      421 cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg accatctgcg
      481 ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc
      541 gtgcctgaga gcgcagtcga attcaagctg ctagcaagga tccaccggtg aggcatggag
      601 gggtctaagg cgtccagcag caccatgcag gtgagcttcg tgtgccagcg ctgtagccag
      661 cctctgaaac tggacacgag cttcaagatc ctggaccggg tcaccatcca ggaactcaca
      721 gctccattac ttaccacagc ccaggcgaaa ccaggagaga cccaggagga agaggctaac
      781 tcaggagagg agccatttat tgaaactcgc caggatggtg tctctcgaag attcatcccc
      841 ccagccagga tgatgtctac agaaagtgct aatagcttca ctctgatcgg ggaggcatct
      901 gatggtggca ccatggagaa cctcagccgg agactcaagg tcactggaga cctgtttgac
      961 atcatgtcgg gccagaccga tgtggatcac ccgctgtgtg aggaatgcac agacactctt
     1021 ttagaccagc tggacactca gctcaatgtc actgagaatg aatgtcagaa ctacaaacgc
     1081 tgtttggaga tcctagagca gatgaatgag gatgacagtg agcagctgca gagggagctg
     1141 aaggagctgg ccttggagga ggagaggctg atccaggagc tggaagatgt ggaaaagaac
     1201 cgcaaggtgg tggcagagaa cctggagaag gtccaggctg aggcggagag attggaccag
     1261 gaggaagctc agtaccagcg ggagtatagt gagtttaaaa ggcagcagct ggagttggat
     1321 gacgaactca agagtgtgga gaaccaggtg cgctacgccc agatccagct ggacaagctc
     1381 aagaaaacca atgtcttcaa tgccaccttc cacatctggc acagcggaca gtttggcaca
     1441 atcaataatt tcagactggg tcgcttgccc agtgttcctg tggagtggaa tgaaatcaat
     1501 gctgcctggg gccagacagt gctgctgctc catgctttgg ccaataagat gggtctgaag
     1561 tttcagaggt accgacttgt tccctatgga aatcattcct atctggagtc tctgacagac
     1621 aaatctaagg agttgccgtt atactgttct gggggtttgc ggtttttctg ggacaacaag
     1681 tttgaccatg caatggtagc ttttctggac tgtgtgcagc agttcaaaga agaggtggaa
     1741 aaaggagaga ctcgattttg tcttccgtac aggatggacg tggagaaagg caagattgaa
     1801 gacactggag gcagtggcgg ctcctattcc atcaaaaccc agtttaactc ggaggagcag
     1861 tggacaaaag cgctcaagtt catgctgacc aatctcaagt ggggtcttgc ctgggtgtcc
     1921 tcacagttct ataacaagta aagcttacta gtggatccgt cgacaatcaa cctctggatt
     1981 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     2041 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     2101 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     2161 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     2221 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     2281 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     2341 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     2401 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     2461 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     2521 cgagtcggat ctccctttgg gccgcctccc cgcctggaat tcgagctcgg tacagcttat
     2581 cgataccgtc gacttcgagc aacttgttta ttgcagctta taatggttac aaataaagca
     2641 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     2701 ccaaactcat caatgtatct tatcatgtct ggatcgtcta gcatcgaaga tcccccgatc
     2761 tgaggaaccc ctagtgatgg agttg
//
