LOCUS       sequence-20962-e         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG414GAL-ccdB-DsRed
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(119..243)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(455..475)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 cggggagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
       61 atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca
      121 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
      181 atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt
      241 ggtgatgggc tgcaggaatt cgatatcaag cttaatggac aacaccgagg acgtcatcaa
      301 ggagttcatg cagttcaagg tgcgcatgga gggctccgtg aacggccact acttcgagat
      361 cgagggcgag ggcgagggca agccctacga gggcacccag accgccaagc tgcaggtgac
      421 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttcc agtacggctc
      481 caaggcctac gtgaagcacc ccgccgacat ccccgactac atgaagctgt ccttccccga
      541 gggcttcacc tgggagcgct ccatgaactt cgaggacggc ggcgtggtgg aggtgcagca
      601 ggactcctcc ctgcaggacg gcaccttcat ctacaaggtg aagttcaagg gcgtgaactt
      661 ccccgccgac ggccccgtaa tg
//
