LOCUS       sequence-209630-       18346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used to generate stable Flp-In cells with 
            tetracycline-inducible human polyduction/fibrocystin expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18346)
  AUTHORS   Kaimori JY, Nagasawa Y, Menezes LF, Garcia-Gonzalez MA, Deng J, Imai
            E, Onuchic LF, Guay-Woodford LM, Germino GG
  TITLE     Polyductin undergoes notch-like processing and regulated release 
            from primary cilia.
  JOURNAL   Hum Mol Genet. 2007 Apr 15. 16(8):942-56.
  PUBMED    17470460
REFERENCE   2  (bases 1 to 18346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2007 Apr 15. 16(8):942-56."
COMMENT     Sequence Label: pcDNA5-PKHD1-myc
FEATURES             Location/Qualifiers
     source          1..18346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          750..882
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     865..884
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        927..945
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          976..1548
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             13982..14011
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(14064..14081)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    14070..14294
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(14427..14446)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    14578..14625
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             14633..15655
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             14633..15655
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    15785..15906
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15822..15841)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15876..15895
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(15955..15971)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15955..15971)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15968..15990)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15979..15995
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(16003..16009,16010..16027,16028..16033))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16048..16069
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(16186..16203)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(16357..16945)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16437..16456)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(17116..17907,17908..17976))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17739..17758
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17977..18081)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(18156..18175)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg taagtatcaa ggttacaaga caggtttaag
      781 gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac
      841 ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca
      901 attacagctc ttaaggctag agtacttaat acgactcact ataggctagt ctggatcgat
      961 cctgagaact tcagggtgag tttggggacc cttgattgtt ctttcttttt cgctattgta
     1021 aaattcatgt tatatggagg gggcaaagtt ttcagggtgt tgtttagaat gggaagatgt
     1081 cccttgtatc accatggacc ctcatgataa ttttgtttct ttcactttct actctgttga
     1141 caaccattgt ctcctcttat tttcttttca ttttctgtaa ctttttcgtt aaactttagc
     1201 ttgcatttgt aacgaatttt taaattcact tttgtttatt tgtcagattg taagtacttt
     1261 ctctaatcac ttttttttca aggcaatcag ggtatattat attgtacttc agcacagttt
     1321 tagagaacaa ttgttataat taaatgataa ggtagaatat ttctgcatat aaattctggc
     1381 tggcgtggaa atattcttat tggtagaaac aactacatcc tggtcatcat cctgcctttc
     1441 tctttatggt tacaatgata tacactgttt gagatgagga taaaatactc tgagtccaaa
     1501 ccgggcccct ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac
     1561 gtgctggtta ttgtactgtc tcatcatttt ggcaaagaat tgatatctgc agaattcgcc
     1621 cttgaaagga tcatttctcc cttgagtcac aaggagacag aaacagaaaa aaaagcacaa
     1681 aagctatgct gctccaatca aaactgaaaa tgcttttaat gtctgagcaa tcttaagagt
     1741 attgatttaa gtggacagaa tgactgcctg gctgatctct ctgatgagta ttgaagtact
     1801 acttttggca gtacgtcacc tgagtttaca tattgaacct gaagaaggta gccttgcagg
     1861 gggaacgtgg atcacagtca tttttgatgg tttggagttg ggtgttcttt accccaacaa
     1921 tggctctcaa ttggagatac acctggtgaa cgtgaacatg gtggtgcccg cattgcggag
     1981 tgttccctgt gacgtctttc ctgttttctt ggatttgcct gtggtgacat gccggaccag
     2041 atctgtgctg tctgaagcac atgagggtct gtacttcctg gaagcatact tcgggggaca
     2101 gctggtaagc agtccaaatc caggaccacg agatagctgt actttcaagt tttccaaggc
     2161 gcagacaccc atcgttcacc aagtttatcc accaagtggt gttccaggaa aactaataca
     2221 tgtatatggc tggattatca ctggaagatt ggaaactttt gattttgatg ctgagtacat
     2281 tgatagccca gtgatcttgg aagctcaagg agacaaatgg gttactcctt gctctcttat
     2341 aaataggcag atgggaagct gttatcctat tcaggaggac catggtcttg ggactctgca
     2401 gtgccatgtg gaaggcgact acatcggctc ccagaatgtt agcttctcag tatttaacaa
     2461 aggaaagtca atggtccaca agaaggcatg gctgatcagt gctaaacagg atcttttcct
     2521 ataccagaca cactcagaaa tattatctgt gtttccagaa actgggagcc ttgggggaag
     2581 aacaaacatc acaattacag gagacttttt tgacaattct gcccaggtta ccattgcagg
     2641 cattccatgt gatattagac acgtgtctcc caggaagatt gagtgcacca ctcgggctcc
     2701 aggaaaagat gtgaggctca ccacccctca gccaggcaat cgagggcttc tttttgaagt
     2761 tggagatgct gttgagggac tggaactgac tgaagccacc ccagggtaca ggtggcagat
     2821 tgtccctaat gccagttctc catttgggtt ttggtcacag gaaggacaac ctttcagagc
     2881 acggctcagt gggttctttg tggctccaga gacaaataat tacactttct ggattcaggc
     2941 agacagccaa gcttccttgc atttcagttg gtcagaggaa ccaaggacta aggtgaaagt
     3001 ggcctccatc agcgtcggca ctgctgactg gtttgactcc tgggagcaga atagggatga
     3061 agggacctgg cagcagaaga ctcccaagtt ggagctgttg ggtggagcca tgtactacct
     3121 ggaagcagag catcatggga tagccccaag cagggggatg aggattggtg tccagattca
     3181 caacacctgg ctgaatcctg atgtggtcac cacttaccta cgggagaagc accagatccg
     3241 agtccgagcc cagaggcttc cagaagtaca ggtgctgaat gtatcaggca gaggaaactt
     3301 cttccttact tgggacaatg tctctagtca gccaatccct gcaaatgcca cagcccatct
     3361 gattcaaaca accattgagg agttacttgc agtaaaatgc aaactggaac ccctttggtc
     3421 taacatcctt ctccggcttg gatttgaacg aggcccagaa gtttccaact ctgatgggga
     3481 cctcaccagt gggacggagc ccttctgtgg caggttcagc ctccgtcagc ctcgacacct
     3541 tgtccttact cccccggctg cccagaaggg ctatcggcta gatcagtata cacacctgtg
     3601 tcttgcatac aaaggccaca tgaacaagat cctgaagatg attgtgtcct tcacaatcgg
     3661 ctttcaaaac atggtaaaga ataccacctg tgactggagt ctcacgagga ccagccccga
     3721 gagctggcag ttcgattgca ctgacctctg ggagacttgt gtgcgttgct tcggggatct
     3781 ccagccccct ccggcaaact cccccgtgct ggttcatcag atcaaccttc tccctctggc
     3841 ccaggagacg ggcctgttct atgtggatga aattattatt gcagacacaa acgtaacagt
     3901 ttctcaagct gattctggaa cggctcgccc agggggcaat ctggtggaat cagtctctgt
     3961 ggtgggatcc cctccggtct acagtgtcac ctcctggctg gcggggtgtg gcacggagct
     4021 cccgctcatc actgcacgct ctgtgcccac tgaaggaaca gaagagggat ctggactggt
     4081 cctggtgacg acacagagac gacagcggac aagtccacct ctaggaggac actttcgcat
     4141 ccagcttcct aatacagtga tttctgatgt ccctgtacaa atttctgctc atcaccttca
     4201 ccagctctta cagaataatg ccgatgactt cacatccagg tacctcaatg ccagtgactt
     4261 cactgtgaag gaggatctat acacttgcta cgaacacgtg tggaccttgt cctggtccac
     4321 tcagattggg gatttgccca attttatcag ggtctctgat gaaaacctta ctggagtgaa
     4381 tcctgctgca gccacgcgtg tggtatatga tggtggagtt tttcttggac ccatatttgg
     4441 agacatgttg gctactgcca accagcatac tcaggtggtt gtgcgagtga atgatgtacc
     4501 agctcattgc ccaggttcct gctctttcca gtacctccaa gggtcaactc cctgtgtcca
     4561 ttctgtgtgg tactccattg atggtgacat caacctaatg atttacatta ccggaactgg
     4621 tttctctggt gactcccagt tcttgcaggt tacagtgaac aaaacgagtt gcaaagttat
     4681 tttctcaaac cagaccaatg tagtctgtca gacagatttg ctacctgttg gaatgcatcg
     4741 gatcttgatg ttggtgagac cctctggtct tgccatcagt gccactggag aagacctctt
     4801 cctaaatgtg aaacctagac tggatatggt ggagccttcc agagctgcgg atattggagg
     4861 gctctgggcc accatccgag gctctagttt ggaaggtgtt agcctgatat tatttggatc
     4921 ttactcgtgt gccatcaatg tcgctacaag caattcaagc agaattcagt gcaaagttcc
     4981 acccaggggg aaagatggac gcattgtgaa tgtgactgtg atcagagggg actattctgc
     5041 agttcttccc agagcattta catatgtctc ttccttaaat ccagttattg tgactctgag
     5101 cagaaacata agcaatatag caggcggtga gaccctggtc attggagtgg cgaggctgat
     5161 gaactatacg gatttggatg tggaagtcca cgtccaggat gccttggctc cggttcacac
     5221 acagtcggct tggggcctgg aggtggcact gcccccactg ccagctggtc tccacagaat
     5281 ttccgtctct atcaatgggg tcagcattca ctcacaaggg gttgatctcc acatccagta
     5341 cctcacagaa gttttcagca tcgagccttg ctgtgggtcc ctgctgggag ggaccatcct
     5401 cagcatctca ggaataggct tcagcaggga cccagctttg gtttgggtac ttgtgggcaa
     5461 tcggtcctgt gacattgtga acttaacgga ggcgagcatc tggtgtgaaa ccctgccagc
     5521 cccccagata cccgatgcgg gcgctcccac tgttccagct gccgtggagg tctgggctgg
     5581 caacaggttc ttcgcccgtg gtccttcacc aagcttggtg gggaaaggct tcaccttcat
     5641 gtatgaagcg gcagcaacac cagtagtcac tgccatgcaa ggagaaatca caaatagcag
     5701 cctgagcctg catgtgggag gaagtaacct ctccaactca gtcatccttc tggggaacct
     5761 gaactgtgat gttgagacac agtccttcca gggcaacgtg agcctgtctg gatgctccat
     5821 ccctcttcac agtctggagg ctggcatcta tcctctccaa gtacgtcaga agcagatggg
     5881 atttgctaat atgtctgtgg tgctccagca atttgcagtg atgcctcgga taatggccat
     5941 cttcccatcg cagggttcgg catgtggtgg gaccatactt actgtgaggg ggttgcttct
     6001 taactctaga aggaggtcag ttcgggttga cctctcgggt ccttttactt gtgtgatttt
     6061 gagtttggga gaccacacca ttctctgcca ggttagcctg gagggtgacc ccttgcctgg
     6121 agcttccttc tccctgaacg tcacagccct ggtcaatggg ctaaccagcg agtgtcaggg
     6181 gaattgcact cttttcataa gggaagaggc aagtcctgtc atggatgcct tgtccacaaa
     6241 caccagtggg tctctgacca ctgtgctgat taggggtcag aggttagcca ccacagctga
     6301 tgagccgatg gtatttgtgg atgatcaact tccttgcaat gtaacttttt ttaatgcaag
     6361 ccacgttgtg tgccagacaa gagacttggc cccaggaccc cactacctgt cagtttttta
     6421 tacaagaaat gggtatgctt gttctggtaa tgtttccaga cacttctaca ttatgcccca
     6481 agtgtttcat tattttccta agaatttcag cttacatggt gggagcctct tgaccataga
     6541 gggcacaggc ctgagaggac agaacaccac gtcagtctat attgaccagc agacctgcct
     6601 gacggtgaac atcggtgctg agctcatccg gtgcattgtt cccacaggga atggctctgt
     6661 tgccctggaa atagaggtag atggactttg gtatcacata ggagtcattg gttataacaa
     6721 ggcctttacc ccagaattaa tctctatttc tcagagcgat gacatcttaa cctttgcagt
     6781 ggcccagatc tcaggagctg caaacattga catttttata ggaatgtcac cctgtgtggg
     6841 tgtctctggt aaccacaccg ttcttcagtg cgtggtccct tcccttccgg ccggggagta
     6901 ccacgtcaga ggctatgact gcatcagagg gtgggcctca tctgccctgg tgttcacctc
     6961 aagagttatt attacagcag tgacggagaa cttcggctgc ctgggtggaa ggctggtgca
     7021 tgtgtttgga gcgggatttt ctccagggaa tgtctcagct gctgtgtgtg gtgctccctg
     7081 ccgagtcctg gctaatgcta cagtgtctgc cttcagctgc ttggttctgc ccctggatgt
     7141 gtccttggcc ttcctgtgtg gcctgaagcg tgaggaggac agctgtgagg ctgccagaca
     7201 cacctatgtg cagtgtgatt tgacagttgc catggcgaca gagcaactgc ttgaatcgtg
     7261 gccttacctc tacatttgcg aggaaagttc ccaatgcctc tttgtgccag atcattgggc
     7321 agagtcaatg tttccatcat tctcgggcct ctttatcagc cctaaagtgg aaagagatga
     7381 agttctcatc tataatagct cctgtaacat taccatggaa actgaggcag agatggagtg
     7441 tgagacgccc aatcagccaa ttactgtcaa gattactgag atacggaaac gctggggcca
     7501 gaacactcag ggcaactttt ctttacagtt ctgccggaga tggtccagga ctcacagctg
     7561 gtttcctgaa aggctgccac aagatggcga caacgtcaca gtggagaatg gccaattgct
     7621 tctgctggac actaacacaa gcatcctcaa cttactgcac attaaagggg gcaagctgat
     7681 tttcatggcc ccaggaccca tcgagctcag ggcacacgcc atccttgttt ctgatggtgg
     7741 agagctccgg attggatccg aagacaagcc cttccaaggc agagctcaga tcacactcta
     7801 cgggagttcc tactcaactc ccttctttcc ctatggagtc aagttcctgg ctgtgaggaa
     7861 tggaactctt tctctgcacg gttcactacc agaagtaatt gtcacctgtc ttagagcaac
     7921 tgcccatgcc ctagacacag tgctggcttt agaagatgct gtggactgga accctgggga
     7981 tgaagttgtc atcatcagtg gaacaggtgt taaaggtgcc aaaccgatgg aagagattgt
     8041 cactgtggaa actgtgcagg atacagacct ctatcttaag tcacctttga gatattctca
     8101 caactttaca gagaattggg tggctggaga gcaccatatt ttaaaggcca ctgtggctct
     8161 gctcagcagg agtattacca tacaaggaaa tctcactaat gagagggaga agctgcttgt
     8221 ttcatgccag gaggccaatg ctccagaagg taatctgcag cactgtttgt attccatgag
     8281 tgagaagatg ctaggatcca gggatatggg agccagagtg atcgttcagt ccttcccaga
     8341 agagcccagc caggtccagt tgaagggagt gcagtttcaa gtcttggggc aagccttcca
     8401 taagcatctg agctcactca ctctggtggg agctatgaga gagtctttca tacagggctg
     8461 cacagtgagg aactccttca gtagaggcct cagcatgtgc gggaccttgg gcctgaaggt
     8521 ggacagtaat gtattctaca atattttagg tcatgcgctg ctagttggga catgcacgga
     8581 gatgagatat atctcctggg aggcaattca tggaaggaaa gatgactggt caggacatgg
     8641 aaatataata agaaacaacg tgatcatcca ggtttctggt gccgagggac tctccaatcc
     8701 tgaaatgttg acaccatctg gcatctatat ctgcagtccc accaatgtta tagaggggaa
     8761 cagagtgtgt ggtgctggct atggctactt tttccatctc atgaccaacc aaacatcaca
     8821 agctccgctt ctttccttca ctcagaacat tgcacattct tgtaccaggt atggtctctt
     8881 tgtataccct aaatttcagc caccttggga taatgtcact ggcaccactc tgttccagag
     8941 cttcacagtt tgggaaagtg caggtggtgc ccagattttt agaagtagca atcttcgcct
     9001 gaaaaacttc aaagtttatt catgcagaga ttttggaatt gacgtcttgg aaagtgatgc
     9061 aaatacttca gttactgaca gcttattact tggtcatttt gcccacaagg gaagtctgtg
     9121 tatgtcatct gggattaaaa ctcctaaaag atgggaactg atggtgtcta acacaacctt
     9181 tgttaatttt gatctcatca actgtgtggc cattagaacc tgttcagact gttcccaagg
     9241 acaaggtgga tttactgtga agaccagcca gttgaagttt acaaactctt caaacttagt
     9301 ggcatttcca tttcctcatg cagcaatttt ggaagacttg gatggatctc tgtctgggaa
     9361 aaacagaagt cacattcttg cttctatgga aaccctttca gcttcttgtt tggtcaattc
     9421 aagctttggt cgggttgtcc atggcagtgc ctgtggagga ggtgttcttt ttcatcgtat
     9481 gtctattggt ttagcgaata ctcctgaagt ttcttatgat ttaaccatga ctgacagcag
     9541 aaataaaaca accactgtca attatgtacg tgatacattg tctaaccctc gtggctggat
     9601 ggctctgctc ttggaccaag agacctactc attgcaatct gagaaccttt ggatcaacag
     9661 atctctgcag tactcagcaa cctttgacaa ctttgctcct ggtaattacc tactgctggt
     9721 gcacacagat ttgccgcctt accctgacat cctcctaaga tgtgggagtc gagtgggtct
     9781 gtcttttcca tttcttccat caccaggtca gaaccaaggc tgtgactggt tcttcaatag
     9841 ccagctgagg caactcacct atctggtttc aggtgaaggc caagttcaag tcattctccg
     9901 ggtgaaggaa ggtatgcccc caactatttc agcttctacc tctgcccctg aatcagcttt
     9961 aaaatggtcc ctccctgaaa catggcaagg tgttgaagaa ggctggggag gatacaacaa
    10021 taccattcca ggccctgggg atgacgttct cattttaccc aacagaactg tccttgtgga
    10081 tacagatctt ccattcttca aagggctgta tgtgatgggg accttagact tccctgtgga
    10141 cagaagcaat gttctgagtg tggcatgcat ggtcattgca ggcggggagc tgaaagttgg
    10201 tactttagaa aatcccttag aaaaggaaca aaagcttctg attctcctta gagcctcaga
    10261 gggagtcttt tgtgaccgta tgaatggaat tcatattgac ccaggaacaa ttggggttta
    10321 tgggaaagtt catctttaca gtgcttatcc taagaactcc tggacacatc ttggagctga
    10381 tattgcctca ggaaatgaga gaattatagt agaagatgca gtggattggc gcccccatga
    10441 caaaatagtc cttagctcct cttcttatga gcctcatgaa gcagaggtcc tcactgtgaa
    10501 agaagtcaag ggccaccatg tgaggatcta tgaacggctc aaacaccggc atattggaag
    10561 tgtacatgtc acggaggatg gccgacacat tcgtttggct gctgaggttg gactgttgac
    10621 ccgaaatata caaattcagc ctgacgtatc atgtaggggg agactgtttg tggggtcctt
    10681 caggaagtcc agccgagaag aattttcagg tgtccttcaa cttcttaatg tggaaattca
    10741 gaacttcggg tcaccattgt actcatctgt tgaattcagt aatgtgtcag caggatcctg
    10801 gatcatatca tctactctgc accagagctg tggcgggggc attcatgcag ctgccagtca
    10861 tggagtactt ttaaatgaca atattgtgtt tggcacagct ggccatggca tagatttaga
    10921 gggtcaggcc tatactgtca ctaataacct tgtggttctg atgacacagc cagcgtggtc
    10981 caccatttgg gtggcaggaa tcaaagtgaa ccaggtaaag gacatcaacc tccatggcaa
    11041 cgttgtggca ggatcagaga gacttggctt tcacatccga ggccacaagt gctcctcttg
    11101 tgaactgctt tggtctgaca atgtggcgca ttcaagtctt catggccttc atctctataa
    11161 ggaaagtgga cttgacaact gtaccagaat ctctggcttc ttggctttca agaactttga
    11221 ctatggtgcc atgttacatg tagagaacag cgtggagata gagaacatta ctctggtaga
    11281 caatactatt ggtcttttgg cagtagtgta tgtattttct gctccacaaa attccgtcaa
    11341 aaaagtgcag attgtgctta ggaattcagt cattgtggcc accagctctt cttttgactg
    11401 cattcaggac aaagtgaagc cgcactcagc caacttgaca tcaacagata gagctccctc
    11461 caatccaaga ggaggtcgaa ttggtattct gtggcctgta ttcacctcag aaccaaatca
    11521 gtggcctcag gagccatggc acaaagtgag gaatgatcat tcaatttcag gaatcatgaa
    11581 acttcaagat gttacctttt ctagttttgt gaagagttgc tatagcgatg acctggatgt
    11641 ctgcattcta ccaaatgcag agaacagtgg aattatgcac ccaataacag cagagaggac
    11701 caggatgcta aagataaaag ataaaaacaa gttctacttt ccttcattac aacccaggaa
    11761 agatttagga aaagtagtct gtcctgaatt agactgtgca agtccaagaa aatatctctt
    11821 caaggatctg gatgggagag ccctgggtct gcctccacca gtttctgtat ttcctaaaac
    11881 agaggcagaa tggactgcat ccttcttcaa cgcaggtaca tttagagaag aacagaaatg
    11941 tacataccaa tttctgatgc aaggattcat ctgcaaacag actgaccaag tggtcctaat
    12001 tcttgatagc gctgatgcca tttgggcaat tcagaagtta tatccagttg tatctgtgac
    12061 tagtggtttt gttgatgtct ttagcagtgt aaatgccaat attccctgct ctacttctgg
    12121 gtcagtgtct actttctatt ctatcttacc catcaggcaa atcaccaaag tctgcttcat
    12181 ggatcaaact cctcaagttt tgcgcttttt tctattgggg aacaaaagta cctccaagct
    12241 tctcttggct gtattctacc atgagctcca gagcccccac gtcttcttag gggaaagttt
    12301 tattccaccc actctggttc agtcagcttc cttattgctg aatgaatcta ttggtgccaa
    12361 ctatttcaac atcatggata acctcttgta tgttgtccta caaggagagg agcccattga
    12421 aatacgctca ggtgtttcca ttcacttggc cctcactgtg atggtttcag tcttagaaaa
    12481 aggctgggaa atagtaatac tcgaaagact aactaacttc ttacagattg gccaaaacca
    12541 aatcaggttt attcacgaga tgcctggcca tgaagagacc ttaaaggcca ttgctgacag
    12601 tagagcaaaa agaaagcgca attgccctac tgtgacttgc actagtcatt atagaagagt
    12661 tggtcaacgt aggcctctca tgatggaaat gaactcacat agggcttcac ccccaatgac
    12721 tgtggaaact atctcaaaag tgattgtcat tgaaattggt gattcgccaa cagtaaggag
    12781 cactggaatg atttcatcct tatcaagtaa caaattacag aatttggctc atcgagtcat
    12841 cactgctcaa cagactgggg tactagagaa tgttctgaat atgactatcg gggccttact
    12901 agttactcag tcaaagggag tcattggcta tggaaataca agcagtttta aaactgggaa
    12961 cttgatatat attcggccct atgcactttc catcctagtc cagccttcag atggagaagt
    13021 gggaaatgag cttccagtgc agccacaatt ggtatttttg gatgagcaga atcgaagagt
    13081 agagtccctg ggacctcctt cagagccatg gacaatttca gcttccctgg aaggagcatc
    13141 agactcagtg ctaaaagggt gcacccaggc agaaactcaa gatggttatg ttagcttcta
    13201 caacttggca gtcttgatct ctgggtcaaa ctggcacttt atttttactg tcacttctcc
    13261 tccaggagtc aattttacag ctcgatccaa gccatttgct gtcttgcctg tgactaggaa
    13321 ggagaagtcg accatcatcc tggctgcttc cctgtcctct gtggcctcat ggctggctct
    13381 gagctgtctg gtgtgctgtt ggcttaaaag aagcaaaagc agaaaaacaa aacctgaaga
    13441 gattcctgaa tcccggacta ataatcaaaa tattcatatc cacatctcat ccaaacgccg
    13501 agaatcacaa gggcccaaaa aagaagacac tgtggtggga gaagatatga gaatgaaggt
    13561 catgctgggc aaggtgaacc agtgccccca ccagttgatg aatggagtgt ccagaaggaa
    13621 agttagccgc cacattgtcc gagaggaaga ggctgctgtg cctgctcctg gtactactgg
    13681 catcacatcc catgggcaca tctgtgcccc aggtgctcct gctcagcagg tgtacctgca
    13741 agagactggg aactggaagg agggccaaga gcagttgctc agataccagc tggcaggcca
    13801 aaatcagctg ctgctgctat gcccagactt cagacaagag aggcagcagt tgccagggca
    13861 aagtcggctg agtaagcaaa gtggcagctt ggggctttcc caagagaaga aagcctcctg
    13921 cggggccact gaggcattct gccttcattc agtacacccg gaaactattc aggagcaact
    13981 ggaacaaaaa ctcatctcag aagaggatct gtgacccggg cggccgctcg agtctagagg
    14041 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
    14101 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
    14161 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
    14221 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
    14281 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
    14341 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
    14401 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
    14461 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
    14521 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtacctagaa
    14581 gttcctattc cgaagttcct attctctaga aagtatagga acttccttgg ccaaaaagcc
    14641 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
    14701 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
    14761 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
    14821 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
    14881 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
    14941 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc
    15001 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
    15061 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
    15121 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
    15181 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
    15241 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
    15301 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
    15361 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc
    15421 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
    15481 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
    15541 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
    15601 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagcacgt
    15661 actacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
    15721 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
    15781 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    15841 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    15901 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
    15961 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    16021 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    16081 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    16141 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    16201 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    16261 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    16321 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    16381 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    16441 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    16501 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    16561 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    16621 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    16681 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    16741 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    16801 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    16861 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    16921 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    16981 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    17041 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    17101 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    17161 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    17221 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    17281 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    17341 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    17401 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    17461 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    17521 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    17581 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    17641 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    17701 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    17761 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    17821 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    17881 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    17941 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    18001 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    18061 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    18121 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
    18181 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
    18241 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
    18301 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgtt
//
