LOCUS       sequence-209648-       13377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing 
            dCas9-VPR and AvrII site for sgRNA insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13377)
  AUTHORS   Schwartz C, Curtis N, Lobs AK, Wheeldon I
  TITLE     Multiplexed CRISPR activation of cryptic sugar metabolism enables 
            Yarrowia lipolytica growth on cellobiose.
  JOURNAL   Biotechnol J. 2018 May 5:e1700584. doi: 10.1002/biot.201700584.
  PUBMED    29729131
REFERENCE   2  (bases 1 to 13377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2018 May 5:e1700584. doi: 10.1002/biot.201700584."
COMMENT     Sequence Label: pCRISPRa_VPR_yl
FEATURES             Location/Qualifiers
     source          1..13377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             join(1358..1384,1385..1387,1388..3874,3875..3877,
                     3878..5461)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5474..5494
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5507..5656
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5675..5695
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6500..7069
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     terminator      7080..7327
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(7096..7114)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(7346..7362)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7346..7362)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7359..7381)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7370..7386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7394..7400,7401..7418,7419..7424))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7439..7460
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7577..7594)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7748..8336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7828..7847)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8507..9298,9299..9367))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9130..9149
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9368..9472)
                     /gene="bla"
                     /label=AmpR promoter
     misc_RNA        9948..10023
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     10073..10091
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(13028..13050)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     13150..13169
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(13363..13377,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(13377,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttcgagctcg gtacccgctt ttcgtagata atggaataca
       61 aatggatatc cagagtatac acatggatag tatacactga cacgacaatt ctgtatctct
      121 ttatgttaac tactgtgagg cgttaaatag agcttgatat ataaaatgtt acatttcaca
      181 gtctgaactt ttgcagatta cctaatttgg taagatatta attatgaact gaaagttgat
      241 ggcatcccta aatttgatga aagggggatc ccccgggttc gaaggtacca aggaagcatg
      301 cggtacccga attcctgagg tgtctcacaa gtgccgtgca gtcccgcccc cacttgcttc
      361 tctttgtgtg tagtgtacgt acattatcga gaccgttgtt cccgcccacc tcgatccggt
      421 ctagactgag gtgtctcaca agtgccgtgc agtcccgccc ccacttgctt ctctttgtgt
      481 gtagtgtacg tacattatcg agaccgttgt tcccgcccac ctcgatccgg ggatccctga
      541 ggtgtctcac aagtgccgtg cagtcccgcc cccacttgct tctctttgtg tgtagtgtac
      601 gtacattatc gagaccgttg ttcccgccca cctcgatccg ggtcgacctg aggtgtctca
      661 caagtgccgt gcagtcccgc ccccacttgc ttctctttgt gtgtagtgta cgtacattat
      721 cgagaccgtt gttcccgccc acctcgatcc gggagctcct gaggtgtctc acaagtgccg
      781 tgcagtcccg cccccacttg cttctctttg tgtgtagtgt acgtacatta tcgagaccgt
      841 tgttcccgcc cacctcgatc cggtctagac tgaggtgtct cacaagtgcc gtgcagtccc
      901 gcccccactt gcttctcttt gtgtgtagtg tacgtacatt atcgagaccg ttgttcccgc
      961 ccacctcgat ccggggatcc ctgaggtgtc tcacaagtgc cgtgcagtcc cgcccccact
     1021 tgcttctctt tgtgtgtagt gtacgtacat tatcgagacc gttgttcccg cccacctcga
     1081 tccgggtcga cctgaggtgt ctcacaagtg ccgtgcagtc ccgcccccac ttgcttctct
     1141 ttgtgtgtag tgtacgtaca ttatcgagac cgttgttccc gcccacctcg atccgggcat
     1201 gcctgcagaa gcttttgtgg ttgggacttt agccaagggt ataaaagacc accgtccccg
     1261 aattaccttt cctcttcttt tctctctctc cttgtcaact cacacccgaa atcgttaagc
     1321 atttccttct gagtataaga atcattcaaa ggcgcgcatg gataagaaat actccattgg
     1381 cctggccatc ggaaccaact ccgtgggttg ggccgtgatc accgatgagt acaaggtgcc
     1441 ctctaagaaa ttcaaggtcc tgggcaacac cgaccgacac tccatcaaga agaacctgat
     1501 cggcgctctg ctcttcgact ctggcgagac cgccgaggcc acccgactga agcgaaccgc
     1561 tcgaagacga tacacccgaa gaaagaaccg aatctgttac ctgcaggaga tcttctctaa
     1621 cgagatggcc aaggtggacg actctttctt ccaccgactg gaggagtctt tcctggtgga
     1681 ggaggacaag aagcacgagc gacaccccat cttcggcaac atcgtggacg aggtggccta
     1741 ccacgagaag taccccacca tctaccacct gcgaaagaag ctggtggact ctaccgacaa
     1801 ggccgacctg cgactgatct acctggccct ggcccacatg atcaagttcc gaggccactt
     1861 cctgatcgag ggcgacctga accccgacaa ctctgacgtg gacaagctgt tcatccagct
     1921 ggtgcagacc tacaaccagc tcttcgaaga gaaccccatt aacgcttctg gcgtggatgc
     1981 taaggccatc ctgtctgccc gactgtctaa gtctcgacga ctcgagaacc tgattgctca
     2041 gctccccgga gagaagaaga acggtctgtt cggaaacctg attgctctgt ccctgggtct
     2101 cacccctaac ttcaagtcca acttcgatct ggctgaggac gctaagctgc agctgtctaa
     2161 ggacacctac gacgatgacc tggataacct gctcgcccag attggcgacc agtacgccga
     2221 cctgttcctg gccgccaaga acctgtctga cgccatcctg ctgtctgaca tcctgcgagt
     2281 gaacaccgag atcaccaagg cccccctgtc tgcctccatg attaagcgat acgatgagca
     2341 ccaccaggat ctgaccctcc tcaaggctct ggtccgacag cagctgcccg agaagtacaa
     2401 ggagattttc ttcgaccagt ctaagaacgg ctacgccggc tacatcgacg gcggcgcctc
     2461 tcaggaggag ttctacaagt tcattaagcc catcctggag aagatggacg gaaccgagga
     2521 actgctcgtg aagctgaacc gagaggacct cctgcgaaag cagcgaacct tcgacaacgg
     2581 ctctatcccc caccagatcc acctgggcga gctgcacgcc atcctgcgac gacaggagga
     2641 cttctacccc ttcctgaagg acaaccgaga gaagatcgag aagatcctga ccttccgaat
     2701 cccctactac gtgggacccc tggcccgagg aaactctcga ttcgcttgga tgacccgaaa
     2761 gtctgaggag accattaccc cctggaactt cgaggaggtg gtggataagg gcgcctctgc
     2821 tcagtctttc atcgagcgaa tgaccaactt cgacaagaac ctccccaacg agaaggtcct
     2881 gcccaagcac tctctgctct acgagtactt caccgtctac aacgagctca ccaaggtcaa
     2941 gtacgtgacc gagggaatgc gaaagcccgc tttcctgtct ggagagcaga agaaggctat
     3001 tgtggatctg ctcttcaaga ctaaccgaaa ggtcaccgtc aagcagctga aggaggatta
     3061 cttcaagaag attgagtgtt tcgattctgt cgagatctcc ggcgtcgagg accgattcaa
     3121 cgcctctctg ggtacctacc acgacctgct gaagattatc aaggacaagg atttcctgga
     3181 taacgaggag aacgaggata ttctcgagga cattgtcctg accctcaccc tgttcgagga
     3241 tcgagagatg attgaggagc gactcaagac ctacgctcac ctgttcgacg acaaggtgat
     3301 gaagcagctg aagcgacgac gatacaccgg ctggggccga ctgtctcgaa agctgatcaa
     3361 cggcatccga gacaagcagt ctggcaagac catcctggac ttcctgaagt ctgacggctt
     3421 cgccaaccga aacttcatgc agctgatcca cgacgactct ctgaccttca aggaggacat
     3481 ccagaaggcc caggtgtctg gccagggcga ctctctgcac gagcacatcg ccaacctggc
     3541 cggctctccc gccattaaga aaggtatcct gcagaccgtc aaggtggtcg atgagctcgt
     3601 caaggtgatg ggccgacaca agcccgagaa cattgtcatt gagatggctc gagagaacca
     3661 gactactcag aagggtcaga aaaactcccg agagcgaatg aagcgaattg aggaaggtat
     3721 taaggagctg ggatcccaga ttctcaagga gcatcccgtg gagaacactc agctccagaa
     3781 cgagaagctg tacctgtact atctgcagaa cggtcgagac atgtacgtcg accaggagct
     3841 ggatatcaac cgactctccg actacgatgt ggacgccatt gtgccccagt ccttcctgaa
     3901 ggacgattct atcgataaca aggtgctgac ccgatccgac aagaaccgag gcaagtctga
     3961 caacgtgccc tccgaggagg tggtcaagaa gatgaagaac tactggcgac agctgctgaa
     4021 cgccaagctg attacccagc gaaagttcga caacctgacc aaggccgagc gaggcggcct
     4081 gtctgagctg gacaaggccg gcttcatcaa gcgacagctg gtggagaccc gacagatcac
     4141 caagcacgtg gcccagatcc tggactctcg aatgaacacc aagtacgacg agaacgacaa
     4201 gctgatccga gaggtgaagg tgatcaccct gaagtctaag ctggtgtctg acttccgaaa
     4261 ggacttccag ttctacaagg tgcgagagat taacaactac caccacgccc acgatgccta
     4321 cctgaacgct gtcgtgggca ccgccctcat caagaagtat cccaagctgg agtccgagtt
     4381 cgtctacggc gactacaagg tctacgatgt gcgaaaaatg attgccaagt ccgagcagga
     4441 gattggcaag gctaccgcca agtacttctt ctactccaac attatgaact tcttcaagac
     4501 cgagattacc ctggctaacg gcgagattcg aaagcgaccc ctcattgaga ccaacggaga
     4561 gaccggtgag atcgtgtggg acaagggacg agacttcgcc accgtgcgaa aggtgctgtc
     4621 tatgccccag gtgaacatcg tgaagaagac cgaggtgcag accggaggtt tctctaagga
     4681 gtccatcctg cccaagcgaa actctgacaa gctgatcgcc cgaaagaagg actgggaccc
     4741 caagaagtac ggaggtttcg actctcccac cgtggcttac tctgtgctgg tggtggccaa
     4801 ggtggagaag ggcaagtcta agaagctgaa gtctgtgaag gagctgctgg gcattaccat
     4861 catggagcga tcttctttcg agaagaaccc cattgacttc ctggaggcca agggatacaa
     4921 ggaggtgaag aaagatctga ttatcaagct ccccaagtac tctctgttcg agctggagaa
     4981 cggacgaaag cgaatgctgg cctctgccgg cgagctgcag aagggaaacg agctggccct
     5041 gccctccaag tacgtcaact tcctgtacct cgcctcccat tacgagaagc tgaagggctc
     5101 tcccgaggat aacgagcaga agcagctctt cgtggagcag cataagcact acctggacga
     5161 gatcatcgag cagatctctg agttctctaa gcgagtgatc ctggctgacg ccaacctgga
     5221 taaggtgctg tctgcttaca acaagcaccg agacaagccc attcgagagc aggctgagaa
     5281 catcattcac ctgttcaccc tgaccaacct gggagccccc gctgccttca agtacttcga
     5341 caccaccatc gaccgaaagc gatacacctc taccaaggag gtgctggacg ccaccctgat
     5401 ccaccagtct atcaccggcc tgtacgagac ccgaatcgac ctgtctcagc tgggcggcga
     5461 ctctcgagcc gaccccaaga agaagcgaaa ggtgggagga tcaggagacg ctctcgatga
     5521 ctttgatctg gacatgcttg gctccgatgc tcttgacgat ttcgacctcg acatgcttgg
     5581 atctgacgct ctggatgact tcgaccttga tatgctcgga tctgatgccc ttgacgattt
     5641 cgatctggac atgctgccgc gatcttctgg ctctcccaag aagaagcgaa aggtgggctc
     5701 tcagtacctg cccgacaccg acgaccgaca ccgaatcgag gagaagcgaa agcgaaccta
     5761 cgagaccttc aagtctatca tgaagaagtc tcccttctct ggccctaccg acccacgacc
     5821 tcctccccga cgaatcgccg tgccctctcg atcttctgcc tctgtgccca agcccgctcc
     5881 tcagccctac cccttcacct cttctctgtc taccatcaac tacgacgagt tccccaccat
     5941 ggtgttcccc tctggccaga tctctcaggc ctctgccctg gcaccagctc ctccacaggt
     6001 gctgcctcag gcacctgcac cggctcctgc accggccatg gtgtctgccc tggcccaagc
     6061 ccctgctccc gtgcctgtgc tggcacctgg ccctccccaa gctgtggctc ctccggctcc
     6121 taagcccacc caggccggcg agggcaccct gtctgaggct ctgctgcagc tgcagttcga
     6181 cgacgaggac ctgggcgccc tgctgggcaa ctctaccgac cccgccgtgt tcaccgacct
     6241 ggcctctgtg gacaactctg agttccagca gctgctgaac cagggcatcc ccgtggctcc
     6301 tcacaccacc gagcccatgc tgatggagta cccccaggcc atcacccgac tggtgaccgg
     6361 cgcccagcga cctccagacc ccgctcctgc ccctctgggc gcccccggcc tgcccaacgg
     6421 cctgctgtct ggcgacgagg acttctcttc tatcgccgac atggacttct ctgccctgct
     6481 gggctctggc tctggctctc gagactctcg agagggcatg ttcctgccca agcccgaggc
     6541 cggctctgcc atctctgacg tgttcgaggg ccgagaggtg tgccagccca agcgaatccg
     6601 acccttccac ccacccggct ctccttgggc taaccgaccc ctgcctgctt ctctggcacc
     6661 aacccccacc ggccctgtgc acgagccagt gggctctctg acccctgctc cagtgcccca
     6721 gcctctggac cctgcccccg ctgtgacccc cgaggcctct cacctgctgg aggaccccga
     6781 cgaggagacc tctcaggccg tgaaggccct gcgagagatg gccgacaccg tgatccccca
     6841 gaaggaggag gccgccatct gcggccagat ggacctgtct caccctccac cccgaggcca
     6901 cctggacgag ctgaccacca ccctggagtc tatgaccgag gacctgaacc tggactctcc
     6961 cctgaccccc gagctgaacg agatcctgga caccttcctg aacgacgagt gcctgctgca
     7021 cgccatgcac atctctaccg gcctgtctat cttcgacacc tctctgttct aagctagcct
     7081 catgtaatta gttatgtcac gcttacattc acgccctccc tccacatccg ctctaaccga
     7141 aaaggaagga gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt
     7201 agtattaaga acgttattta tatttcaaat ttttcttttt tttctgtaca gacgcgtgta
     7261 cgcatgtaac attatactga aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt
     7321 aatttgcaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7381 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7441 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7501 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7561 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7621 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7681 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7741 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7801 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7861 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7921 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7981 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8041 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8101 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8161 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     8221 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8281 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8341 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8401 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8461 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8521 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8581 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8641 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8701 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8761 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8821 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8881 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8941 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9001 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9061 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9121 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9181 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9241 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9301 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9361 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9421 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9481 cccgaaaagt gccacctgac gtccccagtt gcaaaagttg acacaactct agatctgctt
     9541 ccaaatatag aatcataaca agggttaggg tgtgattata taatattggt cttaattgat
     9601 gtgctagggc tttaaaagtt ggttaaaata acgctctaat gcctttttaa tatattgtct
     9661 ttttcaaaat ctcaaatcgg acacttcttc gtgtatgaga ctccattttt tggctccgtc
     9721 acgtgatatg tattatcagc tatagtggtg taaacaaagt tttttactag ctgtaatggc
     9781 attttgtcgg agtggtaaat cgccttcttg ttgtgcgttc gagttctgga ctctgcactg
     9841 ggctactttg aaaaatacct ctaatgcgcc gatggtttag tggtaaaatc catcgttgcc
     9901 atcgatgggc ccccggttcg attccgggtc ggcgcaggtt gacctaggtt ttagagctag
     9961 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
    10021 tgcttttttt tacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    10081 tatcacgagg cccagatcct ctagagtcga agcggccgct atgtctgata aaaggatgta
    10141 acataggcaa gctgctcgtg agtgttgagt acgaacctta gatccaaatc acccgcaccc
    10201 acggatatac ttgcttgaat atacagtagt atgcggccgc ttcgacacca tatcatataa
    10261 aactaacaat gcattgaatt aatacgaaga ctacccgttg ctatctccac accgttatct
    10321 ccacggtcca aaggctgctc aatgtgctgc atacgtaacg tggggtgcaa ccttgagcac
    10381 atagtacttt ccgaaaaccg gcgataatta agtgtgcact ccaacttttc acactgagca
    10441 taaaatgtgg agaagaaatc acactaaaaa gtcaggtaga ctggaaaaat gcgccatgaa
    10501 ataatatctc ttgctacagt aatgcccagc atcgaggggt attgtgtcac caacactata
    10561 gtggcagctg aagcgctcgt gattgtagta tgagtcttta ttggtgatgg gaagagtcca
    10621 ctcaatattc tcgttactgc caaaacacca cggtaatcgg ccagacacca tggatgtaga
    10681 tcaccaagcc tgtgaatgtt attcgagcta aaatgcacat ggttggtgaa aggagtagtt
    10741 gctgtcgaat tccgtcgtcg cctgagtcat catttattta ccagttggcc acaaaccctt
    10801 gacgatctcg tatgtcccct ccgacatact cccggccggc tggggtacgt tcgatagcgc
    10861 tatcggcatc gacaaggttt gggtccctag ccgataccgc actacctgag tcacaatctt
    10921 cggaggttta gtcttccaca tagcacgggc aaaagtgcgt atatatacaa gagcgtttgc
    10981 cagccacaga ttttcactcc acacaccaca tcacacatac aaccacacac atccacaatg
    11041 gaacccgaaa ctaagaagac caagactgac tccaagaaga ttgttcttct cggcggcgac
    11101 ttctgtggcc ccgaggtgat tgccgaggcc gtcaaggtgc tcaagtctgt tgctgaggcc
    11161 tccggcaccg agtttgtgtt tgaggaccga ctcattggag gagctgccat tgagaaggag
    11221 ggcgagccca tcaccgacgc tactctcgac atctgccgaa aggctgactc tattatgctc
    11281 ggtgctgtcg gaggcgctgc caacaccgta tggaccactc ccgacggacg aaccgacgtg
    11341 cgacccgagc agggtctcct caagctgcga aaggacctga acctgtacgc caacctgcga
    11401 ccctgccagc tgctgtcgcc caagctcgcc gatctctccc ccatccgaaa cgttgagggc
    11461 accgacttca tcattgtccg agagctcgtc ggaggtatct actttggaga gcgaaaggag
    11521 gatgacggat ctggcgtcgc ttccgacacc gagacctact ccgttcctga ggttgagcga
    11581 attgcccgaa tggccgcctt cctggccctt cagcacaacc cccctcttcc cgtgtggtct
    11641 cttgacaagg ccaacgtgct ggcctcctct cgactttggc gaaagactgt cactcgagtc
    11701 ctcaaggacg aattccccca gctcgagctc aaccaccagc tgatcgactc ggccgccatg
    11761 atcctcatca agcagccctc caagatgaat ggtatcatca tcaccaccaa catgtttggc
    11821 gatatcatct ccgacgaggc ctccgtcatc cccggttctc tgggtctgct gccctccgcc
    11881 tctctggctt ctctgcccga caccaacgag gcgttcggtc tgtacgagcc ctgtcacgga
    11941 tctgcccccg atctcggcaa gcagaaggtc aaccccattg ccaccattct gtctgccgcc
    12001 atgatgctca agttctctct taacatgaag cccgccggtg acgctgttga ggctgccgtc
    12061 aaggagtccg tcgaggctgg tatcactacc gccgatatcg gaggctcttc ctccacctcc
    12121 gaggtcggag actttgttgc caacaaggtc aaggagctgc tcaagaagga gtaagtcgtt
    12181 tctacgacgc attgatggaa ggagcaaact gacgcgcctg cgggttggtc taccggcagg
    12241 gtccgctagt gtataagact ctataaaaag ggcccagccc tgctaatgaa atgatgattt
    12301 ataatttacc ggtgtagcaa ccttgactag aagaagcaga ttgggtgtgt ttgtagtgga
    12361 ggacagtggt acgttttgga aacagtcttc ttgaaagtgt cttgtctaca gtatattcac
    12421 tcataacctc aatagccaag ggtgtagtcg gtttattaaa ggaagggagt tgtggctgat
    12481 gtggatagat atctttaaag ctggcgactg cacccaacga gtgtggtggt agcttgttac
    12541 tgtatattcg gtaagatata ttttgtgggg tttttagtgg tgtttggtag gttagtgtct
    12601 ggtatatgag ttgtaggcat gacaatttgg aaaggggtgg actttgggaa tattgtggga
    12661 tttcaatacc ttagtttgta cagggtaatt gttacaaatg atacaaagaa ctgtatttct
    12721 tttcatttgt tttaattggt tgtatatcaa gtccgttaga cgagctcagt gccatggctt
    12781 ttggcactgt atttcatttt tagaggtaca ctacatccag tgaggtatgg taaggttgag
    12841 ggcataatga aggcaccttg tactgacagt cacagacctc tcaccgagaa ttttatgaga
    12901 tatactcggg ttcattttag gctccgattc gattcaaatt attactgtcg aaatcggttg
    12961 agcatccgtt gatttccgaa cagatctggc cctttcgtct cgcgcgtttc ggtgatgacg
    13021 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
    13081 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
    13141 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
    13201 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
    13261 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
    13321 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgtt
//
