LOCUS       sequence-209727-       14645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  prp8-G736E in pRS313 backbone for expression in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14645)
  AUTHORS   Mayerle M, Yitiz S, Soulette C, Rogel LE, Ramirez A, Ragle JM, 
            Katzman S, Guthrie C, Zahler AM
  TITLE     Prp8 impacts cryptic but not alternative splicing frequency.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Feb 5;116(6):2193-2199. doi: 
            10.1073/pnas.1819020116. Epub 2019 Jan 23.
  PUBMED    30674666
REFERENCE   2  (bases 1 to 14645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1819020116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-02-5- 5"; volume: "116"; issue: "6"; pages: "2193-2199"
COMMENT     Sequence Label: prp8-G736E
FEATURES             Location/Qualifiers
     source          1..14645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    76..579
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        609..713
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(714..782,783..1574)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(932..951)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1745..2333
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2234..2253
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2487..2504
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2621..2642
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2657..2662,2663..2680,2681..2687)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2695..2711
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2700..2722
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2719..2735
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2719..2735
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2756..2774
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             3577..3594
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(12581..12600)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12582..12600)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12607..12624)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12607..12623)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12616..12638)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12764..13219
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12851..12870)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13061..13082
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(13479..14141)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(14142..14329)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(14476..14495)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     14595..14617
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagggtcc ttttcatcac gtgctataaa aataattata atttaaattt tttaatataa
      121 atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt
      181 ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt
      241 cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg
      301 aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc
      361 ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt
      421 tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat
      481 ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt
      541 aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtcaggtggc acttttcggg
      601 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      661 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      721 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      781 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      841 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      901 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      961 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1021 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1081 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1141 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1201 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1261 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1321 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1381 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1441 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1501 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1561 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1621 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1681 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1741 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1801 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1861 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1921 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1981 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2041 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2101 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2161 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2221 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2281 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2341 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2401 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2461 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2521 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     2581 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     2641 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     2701 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctcgaaatta
     2761 accctcacta aagggaacaa aagctggtac cgggcccccc ctcgagactt caaagcatgg
     2821 tttggtttgg gccaggctta tgctctcctg gacatgcatt tatattctct ttactacttc
     2881 cagaaagctt gcactttgaa accttgggat cgtcggattt ggcaagtatt gggagaatgt
     2941 tatagtaaga cgggaaataa ggtagaagct ataaaatgct acaaaagatc cataaaagct
     3001 tcacaaacgg tcgatcaaaa tacttcaata tattaccggt tagcgcaact atatgaagaa
     3061 cttgaagact tgcaagaatg taagaagttc atgatgaaat gtgtagatgt ggaagaactt
     3121 ctggaaggta tagtaacaga tgaaaccgtg aaggctaggc tttggctggc aatatttgag
     3181 attaaggcag gaaactacca attggcttat gattatgcca tgggggtatc tagtggaacg
     3241 tctcaagaga ttgaagaggc tcgtatgctg gctcgggagt gcagaaggca tatgtagtga
     3301 agtgaacata cacatagcta ttcgtactaa atgatatgaa atttttataa atgccaggct
     3361 atatagctat ttaaagtgac catggcagaa ggatgaaccg aggtaatacg gctagtacaa
     3421 aagcaacaaa gttaggaata caatttgaga aacgaagacc atagaaaata cttgtgcgat
     3481 tgaacttcct tccaaaaaaa aaatagcgtc aaagaaagat gagtggacta ccgcccccac
     3541 ctcctggttt tgaagaggac agcgacttag cacttccacc accaccacca ccaccgcctg
     3601 gatacgaaat cgaagaactg gataatccga tggtgccatc atcggtaaat gaggatacat
     3661 tccttccgcc tccaccacct cctccaagca acttcgaaat aaacgctgaa gaaattgtgg
     3721 acttcacatt accaccgcca ccaccccctc caggtctaga tgaattagag actaaagcag
     3781 aaaagaaagt agaattacat ggaaagagaa aattggacat aggaaaagat actttcgtca
     3841 ctcgaaaatc aagaaagcgt gcaaaaaaaa tgaccaaaaa ggcaaagaga agcaatttat
     3901 atactcctaa agccgagatg ccaccggaac atctgcgaaa gattattaat actcacagtg
     3961 acatggcctc caaaatgtac aatactgata aaaaggcttt tctaggcgct ctaaagtatt
     4021 taccgcacgc gattttaaaa ttgttagaga acatgcctca tccatgggag caagccaaag
     4081 aagttaaagt tttatatcac acttcaggag ccataacatt tgtgaatgaa acaccaagag
     4141 taatagagcc tgtatatact gcccaatggt cagctacttg gatagcaatg aggagagaaa
     4201 aacgagatag aacacatttt aaaagaatga gatttccacc tttcgatgat gacgaaccac
     4261 ctctgtcata tgaacagcac atagaaaata tcgagccact ggatcctata aatcttccct
     4321 tagactctca agatgatgaa tatgttaaag attggctcta cgattcccgg cccttggagg
     4381 aggatagtaa aaaggtcaat ggtacatctt ataaaaagtg gagttttgat ttacctgaaa
     4441 tgtcgaactt atataggtta tcaacgccac ttagagatga agtaactgac aaaaattact
     4501 attatctttt cgataaaaaa tcttttttta acggtaaagc ccttaataac gcaatccctg
     4561 gaggtcctaa atttgaacct ttatacccaa gagaagagga ggaagattat aacgaattca
     4621 actctataga tagagtaata tttagggttc ctattagaag cgaatataag gtagcgtttc
     4681 ctcatctata taattcccgg ccccgttcag tgcgcatacc atggtataat aatccagtgt
     4741 cgtgtattat ccagaacgat gaggagtacg acacgcctgc actttttttt gatccatcgc
     4801 tgaacccaat acctcacttt attgataata attcatccct caacgtatca aatacaaagg
     4861 aaaacggtga tttcacttta cctgaagatt ttgcacctct tttggctgaa gaagaagaac
     4921 ttattttgcc aaatacaaaa gatgcgatgt cgttatatca ttcacctttt ccatttaata
     4981 gaactaaagg aaaaatggtg agagcacaag atgttgccct ggctaaaaaa tggtttttgc
     5041 aacatcctga cgaggagtat cccgttaaag ttaaggtctc ttaccaaaag ctcttgaaga
     5101 actacgtgct aaatgaattg caccctactt tgccaacaaa ccacaacaaa actaagttat
     5161 tgaagagttt aaaaaatacc aagtattttc aacaaactac aattgattgg gtagaggcag
     5221 gactccagct atgtcgacaa ggacacaata tgttaaattt attaatccac agaaagggcc
     5281 taacttattt acatctcgac tacaatttca atttaaaacc aactaaaacc ttaaccacaa
     5341 aagaacgtaa aaagtcaaga cttggaaact catttcattt gatgcgtgaa ttgctaaaaa
     5401 tgatgaaact gattgtagat acacatgtgc agtttaggtt aggaaatgta gatgccttcc
     5461 agttagcaga tggtatacat tatattttga accacatagg acaattgacc ggtatttatc
     5521 gttataaata taaagtaatg catcaaatcc gcgcatgtaa agatctgaaa catatcatat
     5581 attataagtt taataaaaat ttgggaaaag gacctggttg tggattttgg caacctgcgt
     5641 ggagagtgtg gctaaatttc ttaagaggca ctataccact actcgaaaga tatattggaa
     5701 accttatcac acgtcaattt gaagagcgtt ctaatgaaat tgtcaaaact acaactaaac
     5761 aaagacttga cgcctactac gatcttgagt tacgaaactc ggtcatggat gacattttag
     5821 aaatgatgcc tgagagtatt cgacagaaaa aggcaagaac tatactgcaa catttaagtg
     5881 aggcttggag atgttggaag gctaatattc catgggatgt tcctggtatg ccagccccaa
     5941 tcaaaaaaat aattgaacgg tatataaaat ccaaagctga tgcctgggta tctgcagctc
     6001 attacaatcg agaacgtata aagcgtggag ctcacgtcga aaagactatg gttaaaaaaa
     6061 atctggggcg tttaacgaga ctttggatca aaaatgagca ggaacgtcaa aggcaaatac
     6121 agaaaaacgg tcccgaaatt actcccgaag aggctacgac catattttct gttatggttg
     6181 agtggcttga atcaaggagc ttttcaccaa taccatttcc acctttaact tacaaaaatg
     6241 atactaagat attagttctt gccctcgaag acctaaaaga tgtctacgca tcaaaagtac
     6301 gtttaaatgc atctgaaagg gaagaacttg cgttgataga ggaggcttat gataatcctc
     6361 acgatacttt gaacagaatc aaaaagtact tgttgaccca gcgagttttt aagcctgttg
     6421 atataaccat gatggaaaac tatcaaaaca tttcccctgt ttattcagtt gatcccttgg
     6481 aaaagattac cgatgcatat cttgatcagt atttatggta cgaagctgac caacgaaagc
     6541 ttttcccaaa ctggattaaa ccaagtgatt cagagatacc acctcttctg gtatacaagt
     6601 ggactcaggg tataaacaac ctatctgaaa tttgggatgt atccagaggc caatctgcag
     6661 ttttgcttga gactactttg ggtgaaatgg ccgaaaaaat tgactttact ttacttaata
     6721 gattacttcg cctgattgta gatcctaata ttgctgacta tatcactgca aaaaataatg
     6781 ttgttatcaa ctttaaagat atgagtcacg tcaacaaata tggcttaata cgcgggttga
     6841 agttcgcttc tttcatattc caatattacg gactagttat agatctttta ctattgggtc
     6901 aggaaagggc tacagatttg gctggtccag ctaacaatcc aaatgaattt atgcaattca
     6961 agagcaaaga agtagaaaag gcacatccga tcagacttta caccagatat ttagatcgta
     7021 tatatatgct ttttcacttt gaagaagatg agggagagga attgactgat gaatacttgg
     7081 cagagaatcc agatccaaac tttgaaaaca gcatcggata caataataga aaatgctggc
     7141 ccaaagattc gcgcatgagg ctaatacgtc aggacgttaa cctagggcga gcagtgtttt
     7201 gggaaattca aagtagggtg ccaacttcgt taacgtcaat taaatgggaa aatgcttttg
     7261 tttccgtata cagtaaaaac aatccgaact tactcttctc tatgtgtggt tttgaagtca
     7321 gaatattacc aagacaaaga atggaagagg tggtttctaa cgatgaaggt gtttgggatt
     7381 tagttgacga aagaaccaag caaagaactg caaaagcata tttaaaggta tctgaagaag
     7441 aaatcaaaaa atttgacagc agaataagag gcatattgat ggcctccggt tcaaccactt
     7501 ttactaaagt ggccgctaaa tggaacactt cactgatatc ccttttcaca tactttagag
     7561 aagccattgt tgccacggag ccgttattag atatcctggt gaaaggagaa acaagaattc
     7621 aaaaccgtgt caaacttggt ttaaattcga aaatgccaac aagatttcca cctgctgttt
     7681 tttatacacc aaaagaactt ggtgggttgg gtatgattag tgcttctcat attttaattc
     7741 ctgcatctga cttaagttgg tcaaaacaga ccgatactgg aattacgcat tttcgtgctg
     7801 gtatgacgca cgaggatgaa aaattaatcc caactatctt ccgttacatc actacttggg
     7861 aaaatgaatt tttagactct caaagggtgt gggccgagta tgcaacgaaa aggcaggaag
     7921 ctattcagca gaataggagg ttagcttttg aagaacttga aggatcgtgg gatcgtggta
     7981 tacctcgtat tagcacttta tttcaaagag atcgacatac tctagcgtat gacagaggtc
     8041 acagaatccg tagggaattt aaacagtact ctctagagag aaacagcccc ttttggtgga
     8101 caaactccca ccatgacggt aaattatgga atttaaatgc ctatagaact gatgtgattc
     8161 aagccctagg aggtattgaa acaattttgg aacacacttt atttaagggg actggtttta
     8221 attcatggga aggtttgttt tgggaaaagg cgtccgggtt tgaggattcc atgcaattta
     8281 agaagctaac tcatgctcaa agaactggtt taagccaaat tccaaatcgt agatttacct
     8341 tatggtggtc accaactatt aacagagcaa atgtctatgt cggtttcttg gtgcaattgg
     8401 atctgactgg tatcttcctc catggtaaaa ttcctacgtt aaaaatatct ttaattcaaa
     8461 tattccgtgc tcatttgtgg caaaaaatac atgaaagtat agtttttgat atttgtcaaa
     8521 ttctcgatgg tgagctagat gttttgcaaa ttgaatctgt gactaaagaa acagtgcatc
     8581 cccgcaaatc gtacaagatg aattcatctg ccgcggacat aactatggaa agtgttcatg
     8641 aatgggaagt ttcaaaaccc tccctattac atgaaacaaa tgactcattt aagggattga
     8701 ttaccaacaa aatgtggttt gatgtacaat tgagatacgg tgattatgat tctcatgata
     8761 tatctcgtta tgtgagggca aaattcctcg attatacaac agataatgtc agcatgtatc
     8821 cctccccaac aggtgttatg atcggtatcg atttggctta taacatgtat gatgcctatg
     8881 gtaattggtt caacggatta aaacctttga tacaaaatag tatgagaaca attatgaaag
     8941 ctaacccagc attatatgtc cttcgtgaac gtattagaaa aggtcttcaa atatatcaat
     9001 ctagtgtaca ggaaccattt ttaaactcct caaactatgc cgagttattc aataacgaca
     9061 ttaaactctt cgttgacgac actaatgtgt atagagttac tgtccacaag acttttgaag
     9121 ggaacgttgc tacaaaagca attaatggtt gcattttcac tttaaatcca aagactgggc
     9181 atctgtttct gaaaattatc catacctctg tatgggccgg tcaaaaacgt ttaagccaat
     9241 tggccaaatg gaaaacagct gaggaagtta gtgctcttgt gagatctttg cccaaagaag
     9301 agcagccaaa acaaattatc gtcacaagaa aggcaatgct tgatccttta gaggttcaca
     9361 tgcttgactt tcctaacata gcaatcaggc caacagagct gcgactacca ttttcagctg
     9421 caatgtcaat agataaactt tctgatgttg tgatgaaagc tactgaacct cagatggttc
     9481 tgtttaacat ttatgatgat tggttagacc gtatttcatc gtacactgca ttttctagac
     9541 ttacactact tttaagagct ttaaagacga acgaggaaag tgccaaaatg attctactca
     9601 gtgaccccac gatcactatc aaatcgtacc atttatggcc ttcattcact gatgaacaat
     9661 ggatcactat cgaatctcaa atgagagatt tgattttgac tgagtacggc agaaaataca
     9721 atgtcaatat atctgcctta actcaaacgg aaattaaaga tattatattg ggtcaaaata
     9781 tcaaggcacc atctgtgaag agacaaaaaa tggctgaatt agaagctgcc aggtcagaaa
     9841 agcagaacga cgaagaagct gcaggtgcat caactgttat gaaaactaaa actatcaatg
     9901 cgcaaggtga ggagatagtt gtagtagctt ccgctgatta cgaaagtcaa acgtttagtt
     9961 caaagaatga atggagaaaa tctgcaattg ctaatacact attatatttg aggttaaaaa
    10021 acatttatgt ttcggctgat gatttcgtgg aggaacagaa tgtttacgtc cttccaaaaa
    10081 atttattaaa gaagtttatt gagatatccg atgtgaaaat ccaggtggcg gcctttattt
    10141 atggtatgtc agcaaaagat catccgaagg tgaaagaaat caagactgtt gtgctggtac
    10201 ctcaattagg ccacgtagga tccgttcaga taagcaacat ccctgacatc ggagaccttc
    10261 cggatactga aggccttgaa ctactcggtt ggatccatac ccagacagaa gaacttaaat
    10321 tcatggctgc gtctgaagta gcaactcatt ctaaactttt tgcggataag aaaagagact
    10381 gcattgatat ttcaattttt tccaccccgg gatcagtatc attaagcgct tataacctga
    10441 ccgacgaagg ttaccaatgg ggtgaagaaa ataaggatat aatgaacgtg ctatcggaag
    10501 ggtttgagcc tactttcagc acgcatgccc aattacttct ctctgaccgt atcacaggta
    10561 attttataat tccatctgga aacgtttgga actacacatt tatgggaaca gcatttaacc
    10621 aagaaggtga ctacaatttt aaatacggca ttcctttgga attctataat gagatgcatc
    10681 gtccagtaca ctttttacag tttagcgaat tggcggggga cgaagagtta gaggccgaac
    10741 aaatcgatgt atttagctga atatattccc caataaactg gaatctgtta tttcatagat
    10801 atatatgtaa gtataagttt gtatgtataa tattcaacgc attttttatg cccgtcgtag
    10861 ttgctaccta ccacacgatg atgtattata atttcggaac tacttcaaag ctacgctttc
    10921 cgcactatga aaagctataa gtatgtgaaa attggtcatt gactgtgatt ctatttcaaa
    10981 aagtaaccaa aggagataga catcgttcgg acagaaaatg gtccgttgtc actcccattc
    11041 cattatgtcc ttcatataga aattggatat tcttgttttc atttcgcgca ataaagcaat
    11101 tccgtgcggc agaaagaact tatatatatt taactgatct tacgctattt atggcaaaac
    11161 ttgtgttaca tttttgaaga taaagttaca atcattacgg cagcctcaaa acaaaattgg
    11221 gagaaaacat actcaagtga gtactcattt tgtgcaagca aacactgaca attgaagaga
    11281 tcgtcaggat gcccggaacg ccacagaaga acaagaggtc tgcgagtata tctgtttcac
    11341 ctgcgaagaa gacagaggaa aaagaaataa tacaaaatga ttcaaaggcg atattatcta
    11401 agcaaactaa gaggaaaaaa aagtatgctt ttgctcctat aaataactta aatgggaaga
    11461 acaccaaggt atccaatgca agtgtcctaa agtcaatagc cgtttcacaa gtacgaaata
    11521 catcaagaac aaaagatata aacaaagcag ttagcaagag cgtaaagcaa ttaccaaatt
    11581 cacaagttaa accgaaacgg gaaatgtcta atttgagtag gcatcacgat ttcactcaag
    11641 acgaggacgg ccccatggaa gaagtaatat ggaaatattc acctttacaa cgagatatgt
    11701 ccgacaaaac aacaagcgct gccgaatact ctgatgacta cgaagatgtc caaaatccct
    11761 cttctacacc tatagtccct aatcgactga aaactgttct tagtttcaca aatatccaag
    11821 tacccaacgc tgacgtaaat caactcattc aagaaaatgg aaatgagcaa gtgcgtccta
    11881 aaccagcaga aatatctacc agggagtctc tccgtaatat agatgatatt ctcgatgata
    11941 tagagggcga tttaactata aaaccgacga taacgaaatt cagcgatttg ccatcttcac
    12001 ccatcaaggc acccaacgtt gaaaaaaaag cagaggtgaa tgcagaagaa gtagataaaa
    12061 tggattcaac aggagatagc aacgatggcg atgactcttt gatagatatt ttaactcaaa
    12121 agtatgttga gaaacgcaag agtgagagtc agataacaat tcaaggcaac accaatcaaa
    12181 aaagtggagc ccaggaaagt tgtgggaaga atgataacac aaaatcgaga ggagaaattg
    12241 aagatcatga aaatgtagac aatcaagcca aaacaggcaa tgcattttat gagaatgaag
    12301 aagacagtaa ttgccaaaga attaaaaaaa atgaaaaaat agaatacaat tcttcagacg
    12361 aattttcaga cgattcacta attgaactat tgaacgaaac tcaaacacaa gtggaaccta
    12421 acactattga acaagattta gacaaagtgg agaaaatggt ctctgacgat ttgagaattg
    12481 ctactgattc cactttatcc gcatatgctc tcagagctaa gtctggtgct ccaagagacg
    12541 gcgtagtacg attagtaata gtaagcttac ctccaattcg ccctatagtg agtcgtatta
    12601 caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
    12661 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
    12721 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc
    12781 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
    12841 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
    12901 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
    12961 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
    13021 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
    13081 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
    13141 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
    13201 atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
    13261 cacaccgcat agatccgtcg agttcaagag aaaaaaaaag aaaaagcaaa aagaaaaaag
    13321 gaaagcgcgc ctcgttcaga atgacacgta tagaatgatg cattaccttg tcatcttcag
    13381 tatcatactg ttcgtataca tacttactga cattcatagg tatacatata tacacatgta
    13441 tatatatcgt atgctgcagc tttaaataat cggtgtcact acataagaac acctttggtg
    13501 gagggaacat cgttggtacc attgggcgag gtggcttctc ttatggcaac cgcaagagcc
    13561 ttgaacgcac tctcactacg gtgatgatca ttcttgcctc gcagacaatc aacgtggagg
    13621 gtaattctgc tagcctctgc aaagctttca agaaaatgcg ggatcatctc gcaagagaga
    13681 tctcctactt tctccctttg caaaccaagt tcgacaactg cgtacggcct gttcgaaaga
    13741 tctaccaccg ctctggaaag tgcctcatcc aaaggcgcaa atcctgatcc aaaccttttt
    13801 actccacgca cggcccctag ggcctcttta aaagcttgac cgagagcaat cccgcagtct
    13861 tcagtggtgt gatggtcgtc tatgtgtaag tcaccaatgc actcaacgat tagcgaccag
    13921 ccggaatgct tggccagagc atgtatcata tggtccagaa accctatacc tgtgtggacg
    13981 ttaatcactt gcgattgtgt ggcctgttct gctactgctt ctgcctcttt ttctgggaag
    14041 atcgagtgct ctatcgctag gggaccaccc tttaaagaga tcgcaatctg aatcttggtt
    14101 tcatttgtaa tacgctttac tagggctttc tgctctgtca tctttgcctt cgtttatctt
    14161 gcctgctcat tttttagtat attcttcgaa gaaatcacat tactttatat aatgtataat
    14221 tcattatgtg ataatgccaa tcgctaagaa aaaaaaagag tcatccgcta ggtggaaaaa
    14281 aaaaaatgaa aatcattacc gaggcataaa aaaatataga gtgtactaga ggaggccaag
    14341 agtaatagaa aaagaaaatt gcgggaaagg actgtgttat gacttccctg actaatgccg
    14401 tgttcaaacg atacctggca gtgactccta gcgctcacca agctcttaaa acgggaattt
    14461 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
    14521 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
    14581 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
    14641 cgcga
//
