LOCUS       sequence-209734-        2895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human WT alpha synuclein with a C-terminal cysteine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Stephens AD, Fernandez Villegas A, Chung CW, Vanderpoorten O, 
            Pinotsi D, Mela I, Ward E, McCoy TM, Cubitt R, Routh AF, Kaminski 
            CF, Kaminski Schierle GS
  TITLE     alpha-synuclein fibril and synaptic vesicle interactions lead to 
            vesicle destruction and increased uptake into neurons
  JOURNAL   bioRxiv 2022.10.04.510646
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.10.04.510646"
COMMENT     Sequence Label: pT7-7 aSyn C141
FEATURES             Location/Qualifiers
     source          1..2895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     119..136
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     371..393
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     458..477
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        458..476
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             508..530
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(join(1092..1883,1884..1952))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1715..1734
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1953..2057)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2125..2143
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      2272..2860
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2761..2780
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
       61 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
      121 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
      181 gtgcggtatt tcacaccgca taggaagatc ttccggaaga tcttcctatg gtgcactctc
      241 agtacaatct gctctgatgc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
      301 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
      361 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
      421 aggcccagcg attcgaactt ctgatagact tcgaaattaa tacgactcac tatagggaga
      481 ccacaacggt ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg
      541 gatgtattca tgaaaggact ttcaaaggcc aaggagggag ttgtggctgc tgctgagaaa
      601 accaaacagg gtgtggcaga agcagcagga aagacaaaag agggtgttct ctatgtaggc
      661 tccaaaacca aggagggagt ggtgcatggt gtggcaacag tggctgagaa gaccaaagag
      721 caagtgacaa atgttggagg agcagtggtg acgggtgtga cagcagtagc ccagaagaca
      781 gtggagggag cagggagcat tgcagcagcc actggctttg tcaaaaagga ccagttgggc
      841 aagaatgaag aaggagcccc acaggaagga attctggaag atatgcctgt ggatcctgac
      901 aatgaggctt atgaaatgcc ttctgaggaa gggtatcaag actatgaacc tgaagcctgc
      961 taagaaatat ctttgctcca agtttcttga gatctgctga cagatgttcc atccaagctt
     1021 atcgatgata agctgtcaaa catgagaatt aaatcaatct aaagtatata tgagtaaact
     1081 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     1141 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     1201 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     1261 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     1321 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     1381 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     1441 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     1501 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     1561 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     1621 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     1681 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
     1741 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     1801 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     1861 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     1921 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     1981 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2041 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     2101 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
     2161 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     2221 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2281 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2341 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2401 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2461 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2521 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2581 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2641 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2701 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2761 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2821 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2881 tttacggttc ctggc
//
