LOCUS       sequence-209736-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ECFP-EYFP FRET positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Kaminski CF, Rees EJ, Schierle GS
  TITLE     A quantitative protocol for intensity-based live cell FRET imaging.
  JOURNAL   Methods Mol Biol. 2014;1076:445-54. doi: 
            10.1007/978-1-62703-649-8_19.
  PUBMED    24108638
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2014"; volume: "1076:445-54. doi"; pages: " 
            10.1007/978-1-62703-649-8_19"
COMMENT     Sequence Label: pECFP18aaEYFP
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(3..5,6..716)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(306..325)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     653..674
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
       61 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      121 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      181 tcgtgaccac cctgacctgg ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
      241 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
      301 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      361 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
      421 agctggagta caactacatc agccacaacg tctatatcac cgccgacaag cagaagaacg
      481 gcatcaaggc caacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
      541 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
      601 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
      661 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg
      721 gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga gggatctgcc atggtgagca
      781 agggcgagga gctgttcacc gggggtggtg cccatcctgg tcgagctgga cggcgacgta
      841 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      901 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cct
//
