LOCUS       sequence-209751-         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A S6 Kinase biosensor based on fluorescence resonance energy 
            transfer (FRET) and those based on bioluminescence energy transfer 
            (BRET).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Komatsu N, Terai K, Imanishi A, Kamioka Y, Sumiyama K, Jin T, Okada 
            Y, Nagai T, Matsuda M
  TITLE     A platform of BRET-FRET hybrid biosensors for optogenetics, chemical
            screening, and in vivo imaging.
  JOURNAL   Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.1038/s41598-018-27174-x.
  PUBMED    29895862
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-06-12"; volume: "8(1):8984. doi"; pages: " 
            10.1038/s41598-018-27174-x"
COMMENT     Sequence Label: pCAGGS-6015nes
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             243..590
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
ORIGIN
        1 tgtcaaataa gcatttccaa atactactag gagaagacgg taacctttta ttgaatgaca
       61 tttccactaa tgggacctgg ttaaatgggc aaaaagtcga gaagaacagc aatcagttac
      121 tgtcccaagg tgatgaaata accgttggtg taggcgtgga atcagatatt ttatctctgg
      181 tcattttcat aaacgacaaa tttaagcagt gcctggagca gaacaaagtt gatcgcggta
      241 ccagtgctgg tggtagtgct ggtggtagtg ctggtggtag tgctggtggt agtgctggtg
      301 gttccggcag tgctggtggt agtgctggtg gtagtaccag tgctggtggt agtgctggtg
      361 gtagtgctgg tggtagtgct ggtggtagtg ctggtggttc cggcagtgct ggtggtagtg
      421 ctggtggtag taccagtgct ggtggtagtg ctggtggtag tgctggtggt agtgctggtg
      481 gtagtgctgg tggttccggc agtgctggtg gtagtgctgg tggtagtacc agtgctggtg
      541 gtagtgctgg tggtagtgct ggtggtagtg ctggtggtag tgctggtggt tccggaaaca
      601 ggcgaatcag aacacttacg gagcccgacg ttgattttaa tggcggccgc atggtgagca
      661 agggcgagga gctgttcacc ggg
//
