LOCUS       sequence-209768-       14452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vectors used for the inducible expression of tripartite 
            split-sfGFP components in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14452)
  AUTHORS   Liu TY, Chou WC, Chen WY, Chu CY, Dai CY, Wu PY
  TITLE     Detection of membrane protein-protein interaction in planta based on
            dual intein-coupled tripartite split-GFP association.
  JOURNAL   Plant J. 2018 Feb 16. doi: 10.1111/tpj.13874.
  PUBMED    29451720
REFERENCE   2  (bases 1 to 14452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2018 Feb 16. doi: 10.1111/tpj.13874."
COMMENT     Sequence Label: UBQ10:sXVE:S10-(MCS)
FEATURES             Location/Qualifiers
     source          1..14452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      91..802
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  1424..2191
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             2383..3177
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             3476..4624
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    6220..6244
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(6491..6512)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6703..6722
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6847..6869
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6861..6878
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6862..6878
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(6891..7091)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     CDS             complement(7138..7941)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGAWIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7249..7268)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7859..7878
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7991..8170)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        11432..11478
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    12083..12107
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12572..13222
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(13410..13427)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(13661..13680)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            13903..14012
                     /note="incP origin of transfer"
     CDS             14045..14416
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 gattgccaag cacgtcccca tgcgctccat caagaagagc gacttcgcgg agctggtgaa
       61 gtacatcacc gacgagcaag gcaagaccga gcgcctttcc gacgctcacc gggctggttg
      121 ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga
      181 agccgtgtgc gagacaccgc ggccgccggc gttgtggata cctcgcggaa aacttggccc
      241 tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt
      301 tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa
      361 tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca agcctgggga
      421 taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga ggggcgcgat
      481 ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg acatttgagg
      541 ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt ttcggccacc
      601 gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt tttaaccagg
      661 gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc
      721 tcccggcccg ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc ggccgcgaac
      781 ggcctcaccc caaaaatggc agcgctggca gtccttgcca ttgccgggat cggggcagta
      841 acgggatggg cgatcagccc gagcgcgacg cccggaagca ttgacgtgcc gcaggtgctg
      901 gcatcgacat tcagcgacca ggtgccgggc agtgagggcg gcggcctggg tggcggcctg
      961 cccttcactt cggccgtcgg ggcattcacg gacttcatgg cggggccggc aatttttacc
     1021 ttgggcattc ttggcatagt ggtcgcgggt gccgtgctcg tgttcggggg tgcgataaac
     1081 ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac gagaattgga
     1141 cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac gaagaggatg
     1201 aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag ataatatatc
     1261 ttttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat aggcagcgcg
     1321 cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact aatgcttgaa
     1381 accaggacaa taaccttata gcttgtaaat tctatcataa ttgggtaatg actccaactt
     1441 attgatagtg ttttatgttc agataatgcc cgatgacttt gtcatgcagc tccaccgatt
     1501 ttgagaacga cagcgacttc cgtcccagcc gtgccaggtg ctgcctcaga ttcaggttat
     1561 gccgctcaat tcgctgcgta tatcgcttgc tgattacgtg cagctttccc ttcaggcggg
     1621 attcatacag cggccagcca tccgtcatcc atatcaccac gtcaaagggt gacagcaggc
     1681 tcataagacg ccccagcgtc gccatagtgc gttcaccgaa tacgtgcgca acaaccgtct
     1741 tccggagact gtcatacgcg taaaacagcc agcgctggcg cgatttagcc ccgacatagc
     1801 cccactgttc gtccatttcc gcgcagacga tgacgtcact gcccggctgt atgcgcgagg
     1861 ttaccgactg cggcctgagt tttttaagtg acgtaaaatc gtgttgaggc caacgcccat
     1921 aatgcgggct gttgcccggc atccaacgcc attcatggcc atatcaatga ttttctggtg
     1981 cgtaccgggt tgagaagcgg tgtaagtgaa ctgcagttgc catgttttac ggcagtgaga
     2041 gcagagatag cgctgatgtc cggcggtgct tttgccgtta cgcaccaccc cgtcagtagc
     2101 tgaacaggag ggacagctga tagaaacaga agccactgga gcacctcaaa aacaccatca
     2161 tacactaaat cagtaagttg gcagcatcac ccataattgt ggtttcaaaa tcggctccgt
     2221 cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt
     2281 aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg
     2341 gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata
     2401 tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg
     2461 tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg
     2521 gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg
     2581 ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc
     2641 aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa
     2701 agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac
     2761 atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta
     2821 ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa
     2881 gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt
     2941 tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt
     3001 attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg
     3061 tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg
     3121 atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac
     3181 ctagatgtgg cgcaacgatg ccggcgacaa gcaggagcgc accgacttct tccgcatcaa
     3241 gtgttttggc tctcaggccg aggcccacgg caagtatttg ggcaaggggt cgctggtatt
     3301 cgtgcagggc aagattcgga ataccaagta cgagaaggac ggccagacgg tctacgggac
     3361 cgacttcatt gccgataagg tggattatct ggacaccaag gcaccaggcg ggtcaaatca
     3421 ggaataaggg cacattgccc cggcgtgagt cggggcaatc ccgcaaggag ggtgaatgaa
     3481 tcggacgttt gaccggaagg catacaggca agaactgatc gacgcggggt tttccgccga
     3541 ggatgccgaa accatcgcaa gccgcaccgt catgcgtgcg ccccgcgaaa ccttccagtc
     3601 cgtcggctcg atggtccagc aagctacggc caagatcgag cgcgacagcg tgcaactggc
     3661 tccccctgcc ctgcccgcgc catcggccgc cgtggagcgt tcgcgtcgtc tcgaacagga
     3721 ggcggcaggt ttggcgaagt cgatgaccat cgacacgcga ggaactatga cgaccaagaa
     3781 gcgaaaaacc gccggcgagg acctggcaaa acaggtcagc gaggccaagc aggccgcgtt
     3841 gctgaaacac acgaagcagc agatcaagga aatgcagctt tccttgttcg atattgcgcc
     3901 gtggccggac acgatgcgag cgatgccaaa cgacacggcc cgctctgccc tgttcaccac
     3961 gcgcaacaag aaaatcccgc gcgaggcgct gcaaaacaag gtcattttcc acgtcaacaa
     4021 ggacgtgaag atcacctaca ccggcgtcga gctgcgggcc gacgatgacg aactggtgtg
     4081 gcagcaggtg ttggagtacg cgaagcgcac ccctatcggc gagccgatca ccttcacgtt
     4141 ctacgagctt tgccaggacc tgggctggtc gatcaatggc cggtattaca cgaaggccga
     4201 ggaatgcctg tcgcgcctac aggcgacggc gatgggcttc acgtccgacc gcgttgggca
     4261 cctggaatcg gtgtcgctgc tgcaccgctt ccgcgtcctg gaccgtggca agaaaacgtc
     4321 ccgttgccag gtcctgatcg acgaggaaat cgtcgtgctg tttgctggcg accactacac
     4381 gaaattcata tgggagaagt accgcaagct gtcgccgacg gcccgacgga tgttcgacta
     4441 tttcagctcg caccgggagc cgtacccgct caagctggaa accttccgcc tcatgtgcgg
     4501 atcggattcc acccgcgtga agaagtggcg cgagcaggtc ggcgaagcct gcgaagagtt
     4561 gcgaggcagc ggcctggtgg aacacgcctg ggtcaatgat gacctggtgc attgcaaacg
     4621 ctagggcctt gtggggtcag ttccggctgg gggttcagca gccagcgctt tactggcatt
     4681 tcaggaacaa gcgggcactg ctcgacgcac ttgcttcgct cagtatcgct cgggacgcac
     4741 ggcgcgctct acgaactgcc gataaacaga ggattaaaat tgacaattgt gattaaggct
     4801 cagattcgac ggcttggagc ggccgacgtg caggatttcc gcgagatccg attgtcggcc
     4861 ctgaagaaag ctccagagat gttcgggtcc gtttacgagc acgaggagaa aaagcccatg
     4921 gaggcgttcg ctgaacggtt gcgagatgcc gtggcattcg gcgcctacat cgacggcgag
     4981 atcattgggc tgtcggtctt caaacaggag gacggcccca aggacgctca caaggcgcat
     5041 ctgtccggcg ttttcgtgga gcccgaacag cgaggccgag gggtcgccgg tatgctgctg
     5101 cgggcgttgc cggcgggttt attgctcgtg atgatcgtcc gacagattcc aacgggaatc
     5161 tggtggatgc gcatcttcat cctcggcgca cttaatattt cgctattctg gagcttgttg
     5221 tttatttcgg tctaccgcct gccgggcggg gtcgcggcga cggtaggcgc tgtgcagccg
     5281 ctgatggtcg tgttcatctc tgccgctctg ctaggtagcc cgatacgatt gatggcggtc
     5341 ctgggggcta tttgcggaac tgcgggcgtg gcgctgttgg tgttgacacc aaacgcagcg
     5401 ctagatcctg tcggcgtcgc agcgggcctg gcgggggcgg tttccatggc gttcggaacc
     5461 gtgctgaccc gcaagtggca acctcccgtg cctctgctca cctttaccgc ctggcaactg
     5521 gcggccggag gacttctgct cgttccagta gctttagtgt ttgatccgcc aatcccgatg
     5581 cctacaggaa ccaatgttct cggcctggcg tggctcggcc tgatcggagc gggtttaacc
     5641 tacttccttt ggttccgggg gatctcgcga ctcgaaccta cagttgtttc cttactgggc
     5701 tttctcagcc ccagatctgg ggtcgatcag ccggggatgc atcaggccga cagtcggaac
     5761 ttcgggtccc cgacctgtac cattcggtga gcaatggata ggggagttga tatcgtcaac
     5821 gttcacttct aaagaaatag cgccactcag cttcctcagc ggctttatcc agcgatttcc
     5881 tattatgtcg gcatagttct caagatcgac agcctgtcac ggttaagcga gaaatgaata
     5941 agaaggctga taattcggat ctctgcgagg gagatgatat ttgatcacag gcagcaacgc
     6001 tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg
     6061 cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac
     6121 aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt
     6181 tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt
     6241 aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgggg
     6301 tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct
     6361 gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga
     6421 tggtggttcc gaaatcggca aaatccctta taaatcaaaa gaatagcccg agatagggtt
     6481 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     6541 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac ccaaatcaag
     6601 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     6661 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     6721 agcgggcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     6781 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     6841 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attaattccc atcttgaaag
     6901 aaatatagtt taaatattta ttgataaaat aacaagtcag gtattatagt ccaagcaaaa
     6961 acataaattt attgatgcaa gtttaaattc agaaatattt caataactga ttatatcagc
     7021 tggtacattg ccgtagatga aagactgagt gcgatattat gtgtaataca taaattgatg
     7081 atatagctag cttagctcat cgggggatcg atccgtcgaa gctagcttgg gtcccgctca
     7141 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
     7201 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
     7261 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
     7321 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac
     7381 gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag
     7441 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
     7501 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
     7561 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
     7621 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
     7681 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
     7741 tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
     7801 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
     7861 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
     7921 ttgttcaatc caagctccca tgggccctcg actagagtcg agatctggat tgagagtgaa
     7981 tatgagactc taattggata ccgaggggaa tttatggaac gtcagtggag catttttgac
     8041 aagaaatatt tgctagctga tagtgacctt aggcgacttt tgaacgcgca ataatggttt
     8101 ctgacgtatg tgcttagctc attaaactcc agaaacccgc ggctgagtgg ctccttcaac
     8161 gttgcggttc tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca
     8221 taacgtgact cccttaattc tccgctcatg atcttgatcc cctgcgccat cagatccttg
     8281 gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc
     8341 cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg
     8401 taagcccact gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag
     8461 cccagtagct gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtgt
     8521 tccgcttcct ttagcagccc ttgcgccctg agtgcttgcg gcagcgtgaa gcttcgacga
     8581 gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt gtttatcaac
     8641 tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa aacaactttg
     8701 cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt caccgcctta
     8761 gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa aacaataccc
     8821 aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa aaactttctc
     8881 tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct caaaatcttc
     8941 gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat tctgttaatc
     9001 ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt ctcgattagg
     9061 gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat aattactctt
     9121 cgatttgtga tttctatcta gttctggtgt tagtttctag tttgtgcgat cgaatttgtc
     9181 gattaatctg agtttttctg attaacagac tagcccgcgg aaaatgaagg ctcttactgc
     9241 tagacaacaa gaagtttttg atcttattag agatcatatt tctcaaactg gaatgcctcc
     9301 tactagagct gaaattgctc aaagacttgg atttaggtct cctaatgctg ctgaagaaca
     9361 tcttaaggct cttgctagaa agggagttat tgaaattgtt tctggagctt caagaggaat
     9421 tagacttctt caagaagaag aagaaggact tcctcttgtt ggaagagttg ctgctggaga
     9481 accttcttct gctcctccta ctgatgtttc tcttggagat gaacttcatc ttgatggaga
     9541 agatgttgct atggctcatg ctgatgctct tgatgatttt gatcttgata tgcttggaga
     9601 tggagattct cctggacctg gatttactcc tcatgattct gctccttatg gagcacttga
     9661 tatggctgat tttgaatttg aacaaatgtt tactgatgct cttggaattg atgaatatgg
     9721 aggagatcct tctgctggag atatgagagc tgctaatctt tggccttctc ctcttatgat
     9781 taagaggtct aagaagaact ctcttgctct ttctcttact gctgaccaaa tggtttctgc
     9841 tcttcttgat gctgaacctc ctattcttta ttctgaatat gatcctacta gacctttttc
     9901 tgaggcttct atgatgggac ttcttactaa tcttgctgat agagaacttg ttcatatgat
     9961 taattgggct aagagagttc ctggatttgt tgatcttact cttcatgacc aagttcatct
    10021 tcttgaatgt gcttggcttg aaattcttat gattggactt gtttggaggt ctatggaaca
    10081 tcctgttaaa cttctttttg ctcctaatct tcttcttgat agaaatcaag gaaagtgtgt
    10141 tgaaggaatg gttgaaattt ttgatatgct tcttgctact tcttcaagat ttagaatgat
    10201 gaatcttcaa ggagaagaat ttgtttgtct taagtctatt attcttctta attctggagt
    10261 ttatactttt ctttcttcta ctcttaagtc tcttgaagaa aaggatcata ttcatagagt
    10321 tcttgataag attactgata ctcttattca tcttatggct aaggctggac ttactcttca
    10381 acaacaacat caaagacttg ctcaacttct tcttattctt tctcatatta gacatatgtc
    10441 taataaggga atggaacatc tttattctat gaagtgtaag aatgttgttc ctctttatga
    10501 tcttcttctt gaaatgcttg atgctcatag acttcatgct cctacttcaa gaggaggagc
    10561 ttctgttgaa gaaactgacc aatctcatct tgctactgct ggatctactt cttctcattc
    10621 tcttcaaaag tattatatta ctggagaagc tgaaggattt cctgctactg tttgaagatc
    10681 ttgatcaagc tccctggcca attcccagag atgttagctg aaatcatcac taatcagata
    10741 ccaaaatatt caaatggaaa tatcaaatag cttctgtttc atcaaaaatg actcgaccta
    10801 actgagtaag catgcttgtt cgagtattat ggcattggga aaactgtttt tcttgtacca
    10861 tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat
    10921 ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat
    10981 attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa
    11041 acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg
    11101 aggagtaaaa catcccaaac gcggccgcaa ccggtcttgc atccagctgg gctgcaggtc
    11161 gaggctaaaa aactaatcgc attatcatcc cctcgacgta ctgtacatat aaccactggt
    11221 tttatataca gcagtactgt acatataacc actggtttta tatacagcag tcgacgtact
    11281 gtacatataa ccactggttt tatatacagc agtactgtac atataaccac tggttttata
    11341 tacagcagtc gaggtaagat tagatatgga tatgtatatg gatatgtata tggtggtaat
    11401 gccatgtaat atgctcgact ctaggatctt cgcaagaccc ttcctctata taaggaagtt
    11461 catttcattt ggagaggaca cgctgaagct agtcgactct agccaaatct aggggatcca
    11521 tggatttacc agacgaccat tacctgtcaa cacaaactat cctttcgaaa gatctcaacg
    11581 gtacggacgt tgggtctggc ggtggctccg gcgcgcccgg gttaattaac tcgagtctag
    11641 ctaaatcgag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat
    11701 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
    11761 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
    11821 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
    11881 aattatcgcg cgcggtgtca tctatgttac taatcgggga attcagatcg gctgagtggc
    11941 tccttcaacg ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg
    12001 cgggggtcat aacgtgactc ccttaattct ccgctcatga tcagattgtc gtttcccgcc
    12061 ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
    12121 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
    12181 ttgtatgtgc atgccaacca cagggttccc cagatctggc gccggccagc gagacgagca
    12241 agattggccg ccgcccgaaa cgatccgaca gcgcgcccag cacaggtgcg caggcaaatt
    12301 gcaccaacgc atacagcgcc agcagaatgc catagtgggc ggtgacgtcg ttcgagtgaa
    12361 ccagatcgcg caggaggccc ggcagcaccg gcataatcag gccgatgccg acagcgtcga
    12421 gcgcgacagt gctcagaatt acgatcaggg gtatgttggg tttcacgtct ggcctccgga
    12481 ccagcctccg ctggtccgat tgaacgcgcg gattctttat cactgataag ttggtggaca
    12541 tattatgttt atcagtgata aagtgtcaag catgacaaag ttgcagccga atacagtgat
    12601 ccgtgccgcc ctggacctgt tgaacgaggt cggcgtagac ggtctgacga cacgcaaact
    12661 ggcggaacgg ttgggggttc agcagccggc gctttactgg cacttcagga acaagcgggc
    12721 gctgctcgac gcactggccg aagccatgct ggcggagaat catacgcatt cggtgccgag
    12781 agccgacgac gactggcgct catttctgat cgggaatgcc cgcagcttca ggcaggcgct
    12841 gctcgcctac cgcgatggcg cgcgcatcca tgccggcacg cgaccgggcg caccgcagat
    12901 ggaaacggcc gacgcgcagc ttcgcttcct ctgcgaggcg ggtttttcgg ccggggacgc
    12961 cgtcaatgcg ctgatgacaa tcagctactt cactgttggg gccgtgcttg aggagcaggc
    13021 cggcgacagc gatgccggcg agcgcggcgg caccgttgaa caggctccgc tctcgccgct
    13081 gttgcgggcc gcgatagacg ccttcgacga agccggtccg gacgcagcgt tcgagcaggg
    13141 actcgcggtg attgtcgatg gattggcgaa aaggaggctc gttgtcagga acgttgaagg
    13201 accgagaaag ggtgacgatt gatcaggacc gctgccggag cgcaacccac tcactacagc
    13261 agagccatgt agacaacatc ccctccccct ttccaccgcg tcagacgccc gtagcagccc
    13321 gctacgggct ttttcatgcc ctgccctagc gtccaagcct cacggccgcg ctcggcctct
    13381 ctggcggcct tctggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    13441 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    13501 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    13561 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    13621 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    13681 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    13741 gcctttctcc cttcgggaag cgtggcgctt ttccgctgca taaccctgct tcggggtcat
    13801 tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat tttgccaaag
    13861 ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga taggtgaagt
    13921 aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc tggcggtgct
    13981 caacgggaat cctgctctgc gaggctggcc ggctaccgcc ggcgtaacag atgagggcaa
    14041 gcggatggct gatgaaacca agccaaccag gaagggcagc ccacctatca aggtgtactg
    14101 ccttccagac gaacgaagag cgattgagga aaaggcggcg gcggccggca tgagcctgtc
    14161 ggcctacctg ctggccgtcg gccagggcta caaaatcacg ggcgtcgtgg actatgagca
    14221 cgtccgcgag ctggcccgca tcaatggcga cctgggccgc ctgggcggcc tgctgaaact
    14281 ctggctcacc gacgacccgc gcacggcgcg gttcggtgat gccacgatcc tcgccctgct
    14341 ggcgaagatc gaagagaagc aggacgagct tggcaaggtc atgatgggcg tggtccgccc
    14401 gagggcagag ccatgacttt tttagccgct aaaacggccg gggggtgcgc gt
//
