LOCUS       sequence-20977-e         645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG416GPD-ccdB-DsRed
FEATURES             Location/Qualifiers
     source          1..645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(120..244)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(456..476)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
       61 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
      121 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
      181 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
      241 tggtgatggg ctgcaggaat tcgatatcaa gcttaatgga caacaccgag gacgtcatca
      301 aggagttcat gcagttcaag gtgcgcatgg agggctccgt gaacggccac tacttcgaga
      361 tcgagggcga gggcgagggc aagccctacg agggcaccca gaccgccaag ctgcaggtga
      421 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc cagtacggct
      481 ccaaggccta cgtgaagcac cccgccgaca tccccgacta catgaagctg tccttccccg
      541 agggcttcac ctgggagcgc tccatgaact tcgaggacgg cggcgtggtg gaggtgcagc
      601 aggactcctc cctgcaggac ggcaccttca tctacaaggt gaagt
//
