LOCUS       sequence-209770-       11996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vectors used for the constitutive expression of tripartite 
            split-sfGFP components in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11996)
  AUTHORS   Liu TY, Chou WC, Chen WY, Chu CY, Dai CY, Wu PY
  TITLE     Detection of membrane protein-protein interaction in planta based on
            dual intein-coupled tripartite split-GFP association.
  JOURNAL   Plant J. 2018 Feb 16. doi: 10.1111/tpj.13874.
  PUBMED    29451720
REFERENCE   2  (bases 1 to 11996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2018 Feb 16. doi: 10.1111/tpj.13874."
COMMENT     Sequence Label: P16-Delta-S:(MCS)-S11
FEATURES             Location/Qualifiers
     source          1..11996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      73..784
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  1407..2174
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             2366..3160
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             3459..4607
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    6203..6227
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(6474..6495)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6686..6705
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6830..6852
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6844..6861
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6845..6861
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(6874..7074)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     CDS             complement(7121..7924)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGAWIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7232..7251)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7842..7861
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7974..8153)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      9190..9442
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9609..9633
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10098..10748
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(10936..10953)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11187..11206)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            11429..11538
                     /note="incP origin of transfer"
     CDS             11571..11942
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 catgcgctcc atcaagaaga gcgacttcgc ggagctggtg aagtacatca ccgacgagca
       61 aggcaagacc gagcgccttt ccgacgctca ccgggctggt tgccctcgcc gctgggctgg
      121 cggccgtcta tggccctgca aacgcgccag aaacgccgtc gaagccgtgt gcgagacacc
      181 gcggccgccg gcgttgtgga tacctcgcgg aaaacttggc cctcactgac agatgagggg
      241 cggacgttga cacttgaggg gccgactcac ccggcgcggc gttgacagat gaggggcagg
      301 ctcgatttcg gccggcgacg tggagctggc cagcctcgca aatcggcgaa aacgcctgat
      361 tttacgcgag tttcccacag atgatgtgga caagcctggg gataagtgcc ctgcggtatt
      421 gacacttgag gggcgcgact actgacagat gaggggcgcg atccttgaca cttgaggggc
      481 agagtgctga cagatgaggg gcgcacctat tgacatttga ggggctgtcc acaggcagaa
      541 aatccagcat ttgcaagggt ttccgcccgt ttttcggcca ccgctaacct gtcttttaac
      601 ctgcttttaa accaatattt ataaaccttg tttttaacca gggctgcgcc ctgtgcgcgt
      661 gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac cctcccggcc cgctaacgcg
      721 ggcctcccat ccccccaggg gctgcgcccc tcggccgcga acggcctcac cccaaaaatg
      781 gcagcgctgg cagtccttgc cattgccggg atcggggcag taacgggatg ggcgatcagc
      841 ccgagcgcga cgcccggaag cattgacgtg ccgcaggtgc tggcatcgac attcagcgac
      901 caggtgccgg gcagtgaggg cggcggcctg ggtggcggcc tgcccttcac ttcggccgtc
      961 ggggcattca cggacttcat ggcggggccg gcaattttta ccttgggcat tcttggcata
     1021 gtggtcgcgg gtgccgtgct cgtgttcggg ggtgcgataa acccagcgaa ccatttgagg
     1081 tgataggtaa gattataccg aggtatgaaa acgagaattg gacctttaca gaattactct
     1141 atgaagcgcc atatttaaaa agctaccaag acgaagagga tgaagaggat gaggaggcag
     1201 attgccttga atatattgac aatactgata agataatata tcttttatat agaagatatc
     1261 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
     1321 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
     1381 atagcttgta aattctatca taattgggta atgactccaa cttattgata gtgttttatg
     1441 ttcagataat gcccgatgac tttgtcatgc agctccaccg attttgagaa cgacagcgac
     1501 ttccgtccca gccgtgccag gtgctgcctc agattcaggt tatgccgctc aattcgctgc
     1561 gtatatcgct tgctgattac gtgcagcttt cccttcaggc gggattcata cagcggccag
     1621 ccatccgtca tccatatcac cacgtcaaag ggtgacagca ggctcataag acgccccagc
     1681 gtcgccatag tgcgttcacc gaatacgtgc gcaacaaccg tcttccggag actgtcatac
     1741 gcgtaaaaca gccagcgctg gcgcgattta gccccgacat agccccactg ttcgtccatt
     1801 tccgcgcaga cgatgacgtc actgcccggc tgtatgcgcg aggttaccga ctgcggcctg
     1861 agttttttaa gtgacgtaaa atcgtgttga ggccaacgcc cataatgcgg gctgttgccc
     1921 ggcatccaac gccattcatg gccatatcaa tgattttctg gtgcgtaccg ggttgagaag
     1981 cggtgtaagt gaactgcagt tgccatgttt tacggcagtg agagcagaga tagcgctgat
     2041 gtccggcggt gcttttgccg ttacgcacca ccccgtcagt agctgaacag gagggacagc
     2101 tgatagaaac agaagccact ggagcacctc aaaaacacca tcatacacta aatcagtaag
     2161 ttggcagcat cacccataat tgtggtttca aaatcggctc cgtcgatact atgttatacg
     2221 ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt tagaatgcaa
     2281 ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc tttaaatact
     2341 gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg aattgaaaaa
     2401 actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg ctaaggtata
     2461 taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc ggtataaagg
     2521 gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag gaaagctgcc
     2581 tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc tcatgagtga
     2641 ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg aaaagattat
     2701 cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg attgtcccta
     2761 tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata acgatctggc
     2821 cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc gcgagctgta
     2881 tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg gcgacctggg
     2941 agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc ttgggagaag
     3001 cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca gggaggatat
     3061 cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga
     3121 gaaaataaaa tattatattt tactggatga attgttttag tacctagatg tggcgcaacg
     3181 atgccggcga caagcaggag cgcaccgact tcttccgcat caagtgtttt ggctctcagg
     3241 ccgaggccca cggcaagtat ttgggcaagg ggtcgctggt attcgtgcag ggcaagattc
     3301 ggaataccaa gtacgagaag gacggccaga cggtctacgg gaccgacttc attgccgata
     3361 aggtggatta tctggacacc aaggcaccag gcgggtcaaa tcaggaataa gggcacattg
     3421 ccccggcgtg agtcggggca atcccgcaag gagggtgaat gaatcggacg tttgaccgga
     3481 aggcatacag gcaagaactg atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg
     3541 caagccgcac cgtcatgcgt gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc
     3601 agcaagctac ggccaagatc gagcgcgaca gcgtgcaact ggctccccct gccctgcccg
     3661 cgccatcggc cgccgtggag cgttcgcgtc gtctcgaaca ggaggcggca ggtttggcga
     3721 agtcgatgac catcgacacg cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg
     3781 aggacctggc aaaacaggtc agcgaggcca agcaggccgc gttgctgaaa cacacgaagc
     3841 agcagatcaa ggaaatgcag ctttccttgt tcgatattgc gccgtggccg gacacgatgc
     3901 gagcgatgcc aaacgacacg gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc
     3961 cgcgcgaggc gctgcaaaac aaggtcattt tccacgtcaa caaggacgtg aagatcacct
     4021 acaccggcgt cgagctgcgg gccgacgatg acgaactggt gtggcagcag gtgttggagt
     4081 acgcgaagcg cacccctatc ggcgagccga tcaccttcac gttctacgag ctttgccagg
     4141 acctgggctg gtcgatcaat ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc
     4201 tacaggcgac ggcgatgggc ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc
     4261 tgctgcaccg cttccgcgtc ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga
     4321 tcgacgagga aatcgtcgtg ctgtttgctg gcgaccacta cacgaaattc atatgggaga
     4381 agtaccgcaa gctgtcgccg acggcccgac ggatgttcga ctatttcagc tcgcaccggg
     4441 agccgtaccc gctcaagctg gaaaccttcc gcctcatgtg cggatcggat tccacccgcg
     4501 tgaagaagtg gcgcgagcag gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg
     4561 tggaacacgc ctgggtcaat gatgacctgg tgcattgcaa acgctagggc cttgtggggt
     4621 cagttccggc tgggggttca gcagccagcg ctttactggc atttcaggaa caagcgggca
     4681 ctgctcgacg cacttgcttc gctcagtatc gctcgggacg cacggcgcgc tctacgaact
     4741 gccgataaac agaggattaa aattgacaat tgtgattaag gctcagattc gacggcttgg
     4801 agcggccgac gtgcaggatt tccgcgagat ccgattgtcg gccctgaaga aagctccaga
     4861 gatgttcggg tccgtttacg agcacgagga gaaaaagccc atggaggcgt tcgctgaacg
     4921 gttgcgagat gccgtggcat tcggcgccta catcgacggc gagatcattg ggctgtcggt
     4981 cttcaaacag gaggacggcc ccaaggacgc tcacaaggcg catctgtccg gcgttttcgt
     5041 ggagcccgaa cagcgaggcc gaggggtcgc cggtatgctg ctgcgggcgt tgccggcggg
     5101 tttattgctc gtgatgatcg tccgacagat tccaacggga atctggtgga tgcgcatctt
     5161 catcctcggc gcacttaata tttcgctatt ctggagcttg ttgtttattt cggtctaccg
     5221 cctgccgggc ggggtcgcgg cgacggtagg cgctgtgcag ccgctgatgg tcgtgttcat
     5281 ctctgccgct ctgctaggta gcccgatacg attgatggcg gtcctggggg ctatttgcgg
     5341 aactgcgggc gtggcgctgt tggtgttgac accaaacgca gcgctagatc ctgtcggcgt
     5401 cgcagcgggc ctggcggggg cggtttccat ggcgttcgga accgtgctga cccgcaagtg
     5461 gcaacctccc gtgcctctgc tcacctttac cgcctggcaa ctggcggccg gaggacttct
     5521 gctcgttcca gtagctttag tgtttgatcc gccaatcccg atgcctacag gaaccaatgt
     5581 tctcggcctg gcgtggctcg gcctgatcgg agcgggttta acctacttcc tttggttccg
     5641 ggggatctcg cgactcgaac ctacagttgt ttccttactg ggctttctca gccccagatc
     5701 tggggtcgat cagccgggga tgcatcaggc cgacagtcgg aacttcgggt ccccgacctg
     5761 taccattcgg tgagcaatgg ataggggagt tgatatcgtc aacgttcact tctaaagaaa
     5821 tagcgccact cagcttcctc agcggcttta tccagcgatt tcctattatg tcggcatagt
     5881 tctcaagatc gacagcctgt cacggttaag cgagaaatga ataagaaggc tgataattcg
     5941 gatctctgcg agggagatga tatttgatca caggcagcaa cgctctgtca tcgttacaat
     6001 caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc
     6061 ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga
     6121 cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
     6181 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta
     6241 gacaacttaa taacacattg cggacgtttt taatgtactg gggtggtttt tcttttcacc
     6301 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag
     6361 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt tccgaaatcg
     6421 gcaaaatccc ttataaatca aaagaatagc ccgagatagg gttgagtgtt gttccagttt
     6481 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     6541 atcagggcga tggcccacta cgtgaaccat cacccaaatc aagttttttg gggtcgaggt
     6601 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     6661 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gccattcagg
     6721 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     6781 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     6841 cgttgtaaaa cgacggccag tgaattaatt cccatcttga aagaaatata gtttaaatat
     6901 ttattgataa aataacaagt caggtattat agtccaagca aaaacataaa tttattgatg
     6961 caagtttaaa ttcagaaata tttcaataac tgattatatc agctggtaca ttgccgtaga
     7021 tgaaagactg agtgcgatat tatgtgtaat acataaattg atgatatagc tagcttagct
     7081 catcggggga tcgatccgtc gaagctagct tgggtcccgc tcagaagaac tcgtcaagaa
     7141 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     7201 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     7261 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     7321 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     7381 gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     7441 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     7501 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     7561 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     7621 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     7681 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt
     7741 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     7801 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     7861 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atccaagctc
     7921 ccatgggccc tcgactagag tcgagatctg gattgagagt gaatatgaga ctctaattgg
     7981 ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc
     8041 tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag
     8101 ctcattaaac tccagaaacc cgcggctgag tggctccttc aacgttgcgg ttctgtcagt
     8161 tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa
     8221 ttctccgctc atgatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc
     8281 cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt
     8341 tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct
     8401 acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc
     8461 atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt cctttagcag
     8521 cccttgcgcc ctgagtgctt gcggcagcgt gaagctttgg aaccatcttt tgggttcctt
     8581 tcttcttctc tttttttgtt tctctcttaa aagtcttttc tcttttcaag tgcttcaaac
     8641 aaaactaatt tgttataaag ggagtttctc tattttatta tatagcaaaa aacttccaaa
     8701 atttctcatt tctgtgttta accttttcgt acatcagttt taaagcacag agtgctcaat
     8761 gttcttccaa tatcgttatt aataaatttt gattaaattc aatcaaatcg gagttatatt
     8821 accacatgag gttaaagggc catattaaaa agtctgcact tcatatgagc aacaaggctt
     8881 ttatgtcttt atggttgatt tgatggccca tatatgatag ttcaaaggcc catattaaaa
     8941 aatgccctaa catgattcga ttcgtatata aagacgctta atgtgacaag cgaacttgct
     9001 cattagggtt tctcatctac gacggcgtgg gctagaggat ccggcgcgcc cgggttaatt
     9061 aactcgagtc tagtggtgga gggtctggtg gcggatcaac cagtgaaaag cgtgaccaca
     9121 tggtccttct tgagtatgta actgctgctg ggattacaga tgctagctaa atcgagctcg
     9181 aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     9241 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     9301 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     9361 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     9421 gtgtcatcta tgttactaga tcgggaattc agatcggctg agtggctcct tcaacgttgc
     9481 ggttctgtca gttccaaacg taaaacggct tgtcccgcgt catcggcggg ggtcataacg
     9541 tgactccctt aattctccgc tcatgatcag attgtcgttt cccgccttca gtttaaacta
     9601 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     9661 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc
     9721 caaccacagg gttccccaga tctggcgccg gccagcgaga cgagcaagat tggccgccgc
     9781 ccgaaacgat ccgacagcgc gcccagcaca ggtgcgcagg caaattgcac caacgcatac
     9841 agcgccagca gaatgccata gtgggcggtg acgtcgttcg agtgaaccag atcgcgcagg
     9901 aggcccggca gcaccggcat aatcaggccg atgccgacag cgtcgagcgc gacagtgctc
     9961 agaattacga tcaggggtat gttgggtttc acgtctggcc tccggaccag cctccgctgg
    10021 tccgattgaa cgcgcggatt ctttatcact gataagttgg tggacatatt atgtttatca
    10081 gtgataaagt gtcaagcatg acaaagttgc agccgaatac agtgatccgt gccgccctgg
    10141 acctgttgaa cgaggtcggc gtagacggtc tgacgacacg caaactggcg gaacggttgg
    10201 gggttcagca gccggcgctt tactggcact tcaggaacaa gcgggcgctg ctcgacgcac
    10261 tggccgaagc catgctggcg gagaatcata cgcattcggt gccgagagcc gacgacgact
    10321 ggcgctcatt tctgatcggg aatgcccgca gcttcaggca ggcgctgctc gcctaccgcg
    10381 atggcgcgcg catccatgcc ggcacgcgac cgggcgcacc gcagatggaa acggccgacg
    10441 cgcagcttcg cttcctctgc gaggcgggtt tttcggccgg ggacgccgtc aatgcgctga
    10501 tgacaatcag ctacttcact gttggggccg tgcttgagga gcaggccggc gacagcgatg
    10561 ccggcgagcg cggcggcacc gttgaacagg ctccgctctc gccgctgttg cgggccgcga
    10621 tagacgcctt cgacgaagcc ggtccggacg cagcgttcga gcagggactc gcggtgattg
    10681 tcgatggatt ggcgaaaagg aggctcgttg tcaggaacgt tgaaggaccg agaaagggtg
    10741 acgattgatc aggaccgctg ccggagcgca acccactcac tacagcagag ccatgtagac
    10801 aacatcccct ccccctttcc accgcgtcag acgcccgtag cagcccgcta cgggcttttt
    10861 catgccctgc cctagcgtcc aagcctcacg gccgcgctcg gcctctctgg cggccttctg
    10921 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    10981 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    11041 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    11101 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    11161 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    11221 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    11281 gggaagcgtg gcgcttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt
    11341 cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact
    11401 ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga
    11461 gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg
    11521 ctctgcgagg ctggccggct accgccggcg taacagatga gggcaagcgg atggctgatg
    11581 aaaccaagcc aaccaggaag ggcagcccac ctatcaaggt gtactgcctt ccagacgaac
    11641 gaagagcgat tgaggaaaag gcggcggcgg ccggcatgag cctgtcggcc tacctgctgg
    11701 ccgtcggcca gggctacaaa atcacgggcg tcgtggacta tgagcacgtc cgcgagctgg
    11761 cccgcatcaa tggcgacctg ggccgcctgg gcggcctgct gaaactctgg ctcaccgacg
    11821 acccgcgcac ggcgcggttc ggtgatgcca cgatcctcgc cctgctggcg aagatcgaag
    11881 agaagcagga cgagcttggc aaggtcatga tgggcgtggt ccgcccgagg gcagagccat
    11941 gactttttta gccgctaaaa cggccggggg gtgcgcgtga ttgccaagca cgtccc
//
