LOCUS       sequence-209771-       12099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vectors used for the constitutive expression of tripartite 
            split-sfGFP components (with a 3xHA tag) in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12099)
  AUTHORS   Liu TY, Chou WC, Chen WY, Chu CY, Dai CY, Wu PY
  TITLE     Detection of membrane protein-protein interaction in planta based on
            dual intein-coupled tripartite split-GFP association.
  JOURNAL   Plant J. 2018 Feb 16. doi: 10.1111/tpj.13874.
  PUBMED    29451720
REFERENCE   2  (bases 1 to 12099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2018 Feb 16. doi: 10.1111/tpj.13874."
COMMENT     Sequence Label: P16-Delta-S:(MCS)-3xHA-S11
FEATURES             Location/Qualifiers
     source          1..12099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      32..743
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  1366..2133
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             2325..3119
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             3418..4566
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    6162..6186
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(6433..6454)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6645..6664
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6789..6811
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6803..6820
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6804..6820
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(6833..7033)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     CDS             complement(7080..7883)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGAWIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7191..7210)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7801..7820
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7933..8112)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             9041..9130
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      9252..9504
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9671..9695
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10160..10810
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(10998..11015)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11249..11268)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            11491..11600
                     /note="incP origin of transfer"
     CDS             11633..12004
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 agtacatcac cgacgagcaa ggcaagaccg agcgcctttc cgacgctcac cgggctggtt
       61 gccctcgccg ctgggctggc ggccgtctat ggccctgcaa acgcgccaga aacgccgtcg
      121 aagccgtgtg cgagacaccg cggccgccgg cgttgtggat acctcgcgga aaacttggcc
      181 ctcactgaca gatgaggggc ggacgttgac acttgagggg ccgactcacc cggcgcggcg
      241 ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc agcctcgcaa
      301 atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga tgatgtggac aagcctgggg
      361 ataagtgccc tgcggtattg acacttgagg ggcgcgacta ctgacagatg aggggcgcga
      421 tccttgacac ttgaggggca gagtgctgac agatgagggg cgcacctatt gacatttgag
      481 gggctgtcca caggcagaaa atccagcatt tgcaagggtt tccgcccgtt tttcggccac
      541 cgctaacctg tcttttaacc tgcttttaaa ccaatattta taaaccttgt ttttaaccag
      601 ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc ttctcgaacc
      661 ctcccggccc gctaacgcgg gcctcccatc cccccagggg ctgcgcccct cggccgcgaa
      721 cggcctcacc ccaaaaatgg cagcgctggc agtccttgcc attgccggga tcggggcagt
      781 aacgggatgg gcgatcagcc cgagcgcgac gcccggaagc attgacgtgc cgcaggtgct
      841 ggcatcgaca ttcagcgacc aggtgccggg cagtgagggc ggcggcctgg gtggcggcct
      901 gcccttcact tcggccgtcg gggcattcac ggacttcatg gcggggccgg caatttttac
      961 cttgggcatt cttggcatag tggtcgcggg tgccgtgctc gtgttcgggg gtgcgataaa
     1021 cccagcgaac catttgaggt gataggtaag attataccga ggtatgaaaa cgagaattgg
     1081 acctttacag aattactcta tgaagcgcca tatttaaaaa gctaccaaga cgaagaggat
     1141 gaagaggatg aggaggcaga ttgccttgaa tatattgaca atactgataa gataatatat
     1201 cttttatata gaagatatcg ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc
     1261 gcttatcaat atatctatag aatgggcaaa gcataaaaac ttgcatggac taatgcttga
     1321 aacccaggac aataacctta tagcttgtaa attctatcat aattgggtaa tgactccaac
     1381 ttattgatag tgttttatgt tcagataatg cccgatgact ttgtcatgca gctccaccga
     1441 ttttgagaac gacagcgact tccgtcccag ccgtgccagg tgctgcctca gattcaggtt
     1501 atgccgctca attcgctgcg tatatcgctt gctgattacg tgcagctttc ccttcaggcg
     1561 ggattcatac agcggccagc catccgtcat ccatatcacc acgtcaaagg gtgacagcag
     1621 gctcataaga cgccccagcg tcgccatagt gcgttcaccg aatacgtgcg caacaaccgt
     1681 cttccggaga ctgtcatacg cgtaaaacag ccagcgctgg cgcgatttag ccccgacata
     1741 gccccactgt tcgtccattt ccgcgcagac gatgacgtca ctgcccggct gtatgcgcga
     1801 ggttaccgac tgcggcctga gttttttaag tgacgtaaaa tcgtgttgag gccaacgccc
     1861 ataatgcggg ctgttgcccg gcatccaacg ccattcatgg ccatatcaat gattttctgg
     1921 tgcgtaccgg gttgagaagc ggtgtaagtg aactgcagtt gccatgtttt acggcagtga
     1981 gagcagagat agcgctgatg tccggcggtg cttttgccgt tacgcaccac cccgtcagta
     2041 gctgaacagg agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat
     2101 catacactaa atcagtaagt tggcagcatc acccataatt gtggtttcaa aatcggctcc
     2161 gtcgatacta tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat
     2221 ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct
     2281 tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa
     2341 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
     2401 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
     2461 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
     2521 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
     2581 gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
     2641 aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg
     2701 acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact
     2761 tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta
     2821 aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct
     2881 tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct
     2941 ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc
     3001 ggtcgatcag ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg
     3061 ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagt
     3121 acctagatgt ggcgcaacga tgccggcgac aagcaggagc gcaccgactt cttccgcatc
     3181 aagtgttttg gctctcaggc cgaggcccac ggcaagtatt tgggcaaggg gtcgctggta
     3241 ttcgtgcagg gcaagattcg gaataccaag tacgagaagg acggccagac ggtctacggg
     3301 accgacttca ttgccgataa ggtggattat ctggacacca aggcaccagg cgggtcaaat
     3361 caggaataag ggcacattgc cccggcgtga gtcggggcaa tcccgcaagg agggtgaatg
     3421 aatcggacgt ttgaccggaa ggcatacagg caagaactga tcgacgcggg gttttccgcc
     3481 gaggatgccg aaaccatcgc aagccgcacc gtcatgcgtg cgccccgcga aaccttccag
     3541 tccgtcggct cgatggtcca gcaagctacg gccaagatcg agcgcgacag cgtgcaactg
     3601 gctccccctg ccctgcccgc gccatcggcc gccgtggagc gttcgcgtcg tctcgaacag
     3661 gaggcggcag gtttggcgaa gtcgatgacc atcgacacgc gaggaactat gacgaccaag
     3721 aagcgaaaaa ccgccggcga ggacctggca aaacaggtca gcgaggccaa gcaggccgcg
     3781 ttgctgaaac acacgaagca gcagatcaag gaaatgcagc tttccttgtt cgatattgcg
     3841 ccgtggccgg acacgatgcg agcgatgcca aacgacacgg cccgctctgc cctgttcacc
     3901 acgcgcaaca agaaaatccc gcgcgaggcg ctgcaaaaca aggtcatttt ccacgtcaac
     3961 aaggacgtga agatcaccta caccggcgtc gagctgcggg ccgacgatga cgaactggtg
     4021 tggcagcagg tgttggagta cgcgaagcgc acccctatcg gcgagccgat caccttcacg
     4081 ttctacgagc tttgccagga cctgggctgg tcgatcaatg gccggtatta cacgaaggcc
     4141 gaggaatgcc tgtcgcgcct acaggcgacg gcgatgggct tcacgtccga ccgcgttggg
     4201 cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc tggaccgtgg caagaaaacg
     4261 tcccgttgcc aggtcctgat cgacgaggaa atcgtcgtgc tgtttgctgg cgaccactac
     4321 acgaaattca tatgggagaa gtaccgcaag ctgtcgccga cggcccgacg gatgttcgac
     4381 tatttcagct cgcaccggga gccgtacccg ctcaagctgg aaaccttccg cctcatgtgc
     4441 ggatcggatt ccacccgcgt gaagaagtgg cgcgagcagg tcggcgaagc ctgcgaagag
     4501 ttgcgaggca gcggcctggt ggaacacgcc tgggtcaatg atgacctggt gcattgcaaa
     4561 cgctagggcc ttgtggggtc agttccggct gggggttcag cagccagcgc tttactggca
     4621 tttcaggaac aagcgggcac tgctcgacgc acttgcttcg ctcagtatcg ctcgggacgc
     4681 acggcgcgct ctacgaactg ccgataaaca gaggattaaa attgacaatt gtgattaagg
     4741 ctcagattcg acggcttgga gcggccgacg tgcaggattt ccgcgagatc cgattgtcgg
     4801 ccctgaagaa agctccagag atgttcgggt ccgtttacga gcacgaggag aaaaagccca
     4861 tggaggcgtt cgctgaacgg ttgcgagatg ccgtggcatt cggcgcctac atcgacggcg
     4921 agatcattgg gctgtcggtc ttcaaacagg aggacggccc caaggacgct cacaaggcgc
     4981 atctgtccgg cgttttcgtg gagcccgaac agcgaggccg aggggtcgcc ggtatgctgc
     5041 tgcgggcgtt gccggcgggt ttattgctcg tgatgatcgt ccgacagatt ccaacgggaa
     5101 tctggtggat gcgcatcttc atcctcggcg cacttaatat ttcgctattc tggagcttgt
     5161 tgtttatttc ggtctaccgc ctgccgggcg gggtcgcggc gacggtaggc gctgtgcagc
     5221 cgctgatggt cgtgttcatc tctgccgctc tgctaggtag cccgatacga ttgatggcgg
     5281 tcctgggggc tatttgcgga actgcgggcg tggcgctgtt ggtgttgaca ccaaacgcag
     5341 cgctagatcc tgtcggcgtc gcagcgggcc tggcgggggc ggtttccatg gcgttcggaa
     5401 ccgtgctgac ccgcaagtgg caacctcccg tgcctctgct cacctttacc gcctggcaac
     5461 tggcggccgg aggacttctg ctcgttccag tagctttagt gtttgatccg ccaatcccga
     5521 tgcctacagg aaccaatgtt ctcggcctgg cgtggctcgg cctgatcgga gcgggtttaa
     5581 cctacttcct ttggttccgg gggatctcgc gactcgaacc tacagttgtt tccttactgg
     5641 gctttctcag ccccagatct ggggtcgatc agccggggat gcatcaggcc gacagtcgga
     5701 acttcgggtc cccgacctgt accattcggt gagcaatgga taggggagtt gatatcgtca
     5761 acgttcactt ctaaagaaat agcgccactc agcttcctca gcggctttat ccagcgattt
     5821 cctattatgt cggcatagtt ctcaagatcg acagcctgtc acggttaagc gagaaatgaa
     5881 taagaaggct gataattcgg atctctgcga gggagatgat atttgatcac aggcagcaac
     5941 gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc
     6001 ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt
     6061 acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat
     6121 tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt
     6181 gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactgg
     6241 ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc
     6301 ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt
     6361 gatggtggtt ccgaaatcgg caaaatccct tataaatcaa aagaatagcc cgagataggg
     6421 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     6481 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca
     6541 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     6601 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     6661 ggagcgggcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     6721 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     6781 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattaattc ccatcttgaa
     6841 agaaatatag tttaaatatt tattgataaa ataacaagtc aggtattata gtccaagcaa
     6901 aaacataaat ttattgatgc aagtttaaat tcagaaatat ttcaataact gattatatca
     6961 gctggtacat tgccgtagat gaaagactga gtgcgatatt atgtgtaata cataaattga
     7021 tgatatagct agcttagctc atcgggggat cgatccgtcg aagctagctt gggtcccgct
     7081 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     7141 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     7201 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     7261 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     7321 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     7381 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     7441 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     7501 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     7561 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     7621 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     7681 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     7741 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     7801 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     7861 tcttgttcaa tccaagctcc catgggccct cgactagagt cgagatctgg attgagagtg
     7921 aatatgagac tctaattgga taccgagggg aatttatgga acgtcagtgg agcatttttg
     7981 acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg caataatggt
     8041 ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctgagt ggctccttca
     8101 acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt
     8161 cataacgtga ctcccttaat tctccgctca tgatcttgat cccctgcgcc atcagatcct
     8221 tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc
     8281 cccagctggc aattccggtt cgcttgctgt ccataaaacc gcccagtcta gctatcgcca
     8341 tgtaagccca ctgcaagcta cctgctttct ctttgcgctt gcgttttccc ttgtccagat
     8401 agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg ctttctacgt
     8461 gttccgcttc ctttagcagc ccttgcgccc tgagtgcttg cggcagcgtg aagctttgga
     8521 accatctttt gggttccttt cttcttctct ttttttgttt ctctcttaaa agtcttttct
     8581 cttttcaagt gcttcaaaca aaactaattt gttataaagg gagtttctct attttattat
     8641 atagcaaaaa acttccaaaa tttctcattt ctgtgtttaa ccttttcgta catcagtttt
     8701 aaagcacaga gtgctcaatg ttcttccaat atcgttatta ataaattttg attaaattca
     8761 atcaaatcgg agttatatta ccacatgagg ttaaagggcc atattaaaaa gtctgcactt
     8821 catatgagca acaaggcttt tatgtcttta tggttgattt gatggcccat atatgatagt
     8881 tcaaaggccc atattaaaaa atgccctaac atgattcgat tcgtatataa agacgcttaa
     8941 tgtgacaagc gaacttgctc attagggttt ctcatctacg acggcgtggg ctagaggatc
     9001 cggcgcgccc gggttaatta actcgagtct agagtcgact tacccatacg atgttccaga
     9061 ttacgctggc tacccatacg atgttccaga ttacgctgga tcatacccat acgatgttcc
     9121 agattacgct actagtggtg gagggtctgg tggcggatca accagtgaaa agcgtgacca
     9181 catggtcctt cttgagtatg taactgctgc tgggattaca gatgctagct aaatcgagct
     9241 cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     9301 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     9361 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     9421 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     9481 cggtgtcatc tatgttacta gatcgggaat tcagatcggc tgagtggctc cttcaacgtt
     9541 gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa
     9601 cgtgactccc ttaattctcc gctcatgatc agattgtcgt ttcccgcctt cagtttaaac
     9661 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
     9721 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat
     9781 gccaaccaca gggttcccca gatctggcgc cggccagcga gacgagcaag attggccgcc
     9841 gcccgaaacg atccgacagc gcgcccagca caggtgcgca ggcaaattgc accaacgcat
     9901 acagcgccag cagaatgcca tagtgggcgg tgacgtcgtt cgagtgaacc agatcgcgca
     9961 ggaggcccgg cagcaccggc ataatcaggc cgatgccgac agcgtcgagc gcgacagtgc
    10021 tcagaattac gatcaggggt atgttgggtt tcacgtctgg cctccggacc agcctccgct
    10081 ggtccgattg aacgcgcgga ttctttatca ctgataagtt ggtggacata ttatgtttat
    10141 cagtgataaa gtgtcaagca tgacaaagtt gcagccgaat acagtgatcc gtgccgccct
    10201 ggacctgttg aacgaggtcg gcgtagacgg tctgacgaca cgcaaactgg cggaacggtt
    10261 gggggttcag cagccggcgc tttactggca cttcaggaac aagcgggcgc tgctcgacgc
    10321 actggccgaa gccatgctgg cggagaatca tacgcattcg gtgccgagag ccgacgacga
    10381 ctggcgctca tttctgatcg ggaatgcccg cagcttcagg caggcgctgc tcgcctaccg
    10441 cgatggcgcg cgcatccatg ccggcacgcg accgggcgca ccgcagatgg aaacggccga
    10501 cgcgcagctt cgcttcctct gcgaggcggg tttttcggcc ggggacgccg tcaatgcgct
    10561 gatgacaatc agctacttca ctgttggggc cgtgcttgag gagcaggccg gcgacagcga
    10621 tgccggcgag cgcggcggca ccgttgaaca ggctccgctc tcgccgctgt tgcgggccgc
    10681 gatagacgcc ttcgacgaag ccggtccgga cgcagcgttc gagcagggac tcgcggtgat
    10741 tgtcgatgga ttggcgaaaa ggaggctcgt tgtcaggaac gttgaaggac cgagaaaggg
    10801 tgacgattga tcaggaccgc tgccggagcg caacccactc actacagcag agccatgtag
    10861 acaacatccc ctcccccttt ccaccgcgtc agacgcccgt agcagcccgc tacgggcttt
    10921 ttcatgccct gccctagcgt ccaagcctca cggccgcgct cggcctctct ggcggccttc
    10981 tggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    11041 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    11101 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    11161 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    11221 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    11281 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    11341 tcgggaagcg tggcgctttt ccgctgcata accctgcttc ggggtcatta tagcgatttt
    11401 ttcggtatat ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga
    11461 ctttccttgg tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc
    11521 gagcgggtgt tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc
    11581 tgctctgcga ggctggccgg ctaccgccgg cgtaacagat gagggcaagc ggatggctga
    11641 tgaaaccaag ccaaccagga agggcagccc acctatcaag gtgtactgcc ttccagacga
    11701 acgaagagcg attgaggaaa aggcggcggc ggccggcatg agcctgtcgg cctacctgct
    11761 ggccgtcggc cagggctaca aaatcacggg cgtcgtggac tatgagcacg tccgcgagct
    11821 ggcccgcatc aatggcgacc tgggccgcct gggcggcctg ctgaaactct ggctcaccga
    11881 cgacccgcgc acggcgcggt tcggtgatgc cacgatcctc gccctgctgg cgaagatcga
    11941 agagaagcag gacgagcttg gcaaggtcat gatgggcgtg gtccgcccga gggcagagcc
    12001 atgacttttt tagccgctaa aacggccggg gggtgcgcgt gattgccaag cacgtcccca
    12061 tgcgctccat caagaagagc gacttcgcgg agctggtga
//
