LOCUS       sequence-209772-       12492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vectors used for the constitutive expression of tripartite 
            split-sfGFP components (GFP1-9) in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12492)
  AUTHORS   Liu TY, Chou WC, Chen WY, Chu CY, Dai CY, Wu PY
  TITLE     Detection of membrane protein-protein interaction in planta based on
            dual intein-coupled tripartite split-GFP association.
  JOURNAL   Plant J. 2018 Feb 16. doi: 10.1111/tpj.13874.
  PUBMED    29451720
REFERENCE   2  (bases 1 to 12492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2018 Feb 16. doi: 10.1111/tpj.13874."
COMMENT     Sequence Label: P16-Delta-S:GFP1–9
FEATURES             Location/Qualifiers
     source          1..12492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  503..1270
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             1462..2256
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             2555..3703
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    5299..5323
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(5570..5591)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5782..5801
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5926..5948
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5940..5957
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5941..5957
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(5970..6170)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     CDS             complement(6217..7020)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGAWIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6328..6347)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6938..6957
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7070..7249)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      8782..9034
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9201..9225
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9690..10340
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(10528..10545)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10779..10798)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            11021..11130
                     /note="incP origin of transfer"
     CDS             11163..11534
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      11661..12372
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tgccgggcag tgagggcggc ggcctgggtg gcggcctgcc cttcacttcg gccgtcgggg
       61 cattcacgga cttcatggcg gggccggcaa tttttacctt gggcattctt ggcatagtgg
      121 tcgcgggtgc cgtgctcgtg ttcgggggtg cgataaaccc agcgaaccat ttgaggtgat
      181 aggtaagatt ataccgaggt atgaaaacga gaattggacc tttacagaat tactctatga
      241 agcgccatat ttaaaaagct accaagacga agaggatgaa gaggatgagg aggcagattg
      301 ccttgaatat attgacaata ctgataagat aatatatctt ttatatagaa gatatcgccg
      361 tatgtaagga tttcaggggg caaggcatag gcagcgcgct tatcaatata tctatagaat
      421 gggcaaagca taaaaacttg catggactaa tgcttgaaac ccaggacaat aaccttatag
      481 cttgtaaatt ctatcataat tgggtaatga ctccaactta ttgatagtgt tttatgttca
      541 gataatgccc gatgactttg tcatgcagct ccaccgattt tgagaacgac agcgacttcc
      601 gtcccagccg tgccaggtgc tgcctcagat tcaggttatg ccgctcaatt cgctgcgtat
      661 atcgcttgct gattacgtgc agctttccct tcaggcggga ttcatacagc ggccagccat
      721 ccgtcatcca tatcaccacg tcaaagggtg acagcaggct cataagacgc cccagcgtcg
      781 ccatagtgcg ttcaccgaat acgtgcgcaa caaccgtctt ccggagactg tcatacgcgt
      841 aaaacagcca gcgctggcgc gatttagccc cgacatagcc ccactgttcg tccatttccg
      901 cgcagacgat gacgtcactg cccggctgta tgcgcgaggt taccgactgc ggcctgagtt
      961 ttttaagtga cgtaaaatcg tgttgaggcc aacgcccata atgcgggctg ttgcccggca
     1021 tccaacgcca ttcatggcca tatcaatgat tttctggtgc gtaccgggtt gagaagcggt
     1081 gtaagtgaac tgcagttgcc atgttttacg gcagtgagag cagagatagc gctgatgtcc
     1141 ggcggtgctt ttgccgttac gcaccacccc gtcagtagct gaacaggagg gacagctgat
     1201 agaaacagaa gccactggag cacctcaaaa acaccatcat acactaaatc agtaagttgg
     1261 cagcatcacc cataattgtg gtttcaaaat cggctccgtc gatactatgt tatacgccaa
     1321 ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa
     1381 cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta aatactgtag
     1441 aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt gaaaaaactg
     1501 atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag
     1561 ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc
     1621 acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt
     1681 ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc
     1741 gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag
     1801 ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg
     1861 aatagcttag acagccgctt agccgaattg gattacttac tgaataacga tctggccgat
     1921 gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga gctgtatgat
     1981 tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac
     2041 agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc
     2101 agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga ggatatcggg
     2161 gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa
     2221 ataaaatatt atattttact ggatgaattg ttttagtacc tagatgtggc gcaacgatgc
     2281 cggcgacaag caggagcgca ccgacttctt ccgcatcaag tgttttggct ctcaggccga
     2341 ggcccacggc aagtatttgg gcaaggggtc gctggtattc gtgcagggca agattcggaa
     2401 taccaagtac gagaaggacg gccagacggt ctacgggacc gacttcattg ccgataaggt
     2461 ggattatctg gacaccaagg caccaggcgg gtcaaatcag gaataagggc acattgcccc
     2521 ggcgtgagtc ggggcaatcc cgcaaggagg gtgaatgaat cggacgtttg accggaaggc
     2581 atacaggcaa gaactgatcg acgcggggtt ttccgccgag gatgccgaaa ccatcgcaag
     2641 ccgcaccgtc atgcgtgcgc cccgcgaaac cttccagtcc gtcggctcga tggtccagca
     2701 agctacggcc aagatcgagc gcgacagcgt gcaactggct ccccctgccc tgcccgcgcc
     2761 atcggccgcc gtggagcgtt cgcgtcgtct cgaacaggag gcggcaggtt tggcgaagtc
     2821 gatgaccatc gacacgcgag gaactatgac gaccaagaag cgaaaaaccg ccggcgagga
     2881 cctggcaaaa caggtcagcg aggccaagca ggccgcgttg ctgaaacaca cgaagcagca
     2941 gatcaaggaa atgcagcttt ccttgttcga tattgcgccg tggccggaca cgatgcgagc
     3001 gatgccaaac gacacggccc gctctgccct gttcaccacg cgcaacaaga aaatcccgcg
     3061 cgaggcgctg caaaacaagg tcattttcca cgtcaacaag gacgtgaaga tcacctacac
     3121 cggcgtcgag ctgcgggccg acgatgacga actggtgtgg cagcaggtgt tggagtacgc
     3181 gaagcgcacc cctatcggcg agccgatcac cttcacgttc tacgagcttt gccaggacct
     3241 gggctggtcg atcaatggcc ggtattacac gaaggccgag gaatgcctgt cgcgcctaca
     3301 ggcgacggcg atgggcttca cgtccgaccg cgttgggcac ctggaatcgg tgtcgctgct
     3361 gcaccgcttc cgcgtcctgg accgtggcaa gaaaacgtcc cgttgccagg tcctgatcga
     3421 cgaggaaatc gtcgtgctgt ttgctggcga ccactacacg aaattcatat gggagaagta
     3481 ccgcaagctg tcgccgacgg cccgacggat gttcgactat ttcagctcgc accgggagcc
     3541 gtacccgctc aagctggaaa ccttccgcct catgtgcgga tcggattcca cccgcgtgaa
     3601 gaagtggcgc gagcaggtcg gcgaagcctg cgaagagttg cgaggcagcg gcctggtgga
     3661 acacgcctgg gtcaatgatg acctggtgca ttgcaaacgc tagggccttg tggggtcagt
     3721 tccggctggg ggttcagcag ccagcgcttt actggcattt caggaacaag cgggcactgc
     3781 tcgacgcact tgcttcgctc agtatcgctc gggacgcacg gcgcgctcta cgaactgccg
     3841 ataaacagag gattaaaatt gacaattgtg attaaggctc agattcgacg gcttggagcg
     3901 gccgacgtgc aggatttccg cgagatccga ttgtcggccc tgaagaaagc tccagagatg
     3961 ttcgggtccg tttacgagca cgaggagaaa aagcccatgg aggcgttcgc tgaacggttg
     4021 cgagatgccg tggcattcgg cgcctacatc gacggcgaga tcattgggct gtcggtcttc
     4081 aaacaggagg acggccccaa ggacgctcac aaggcgcatc tgtccggcgt tttcgtggag
     4141 cccgaacagc gaggccgagg ggtcgccggt atgctgctgc gggcgttgcc ggcgggttta
     4201 ttgctcgtga tgatcgtccg acagattcca acgggaatct ggtggatgcg catcttcatc
     4261 ctcggcgcac ttaatatttc gctattctgg agcttgttgt ttatttcggt ctaccgcctg
     4321 ccgggcgggg tcgcggcgac ggtaggcgct gtgcagccgc tgatggtcgt gttcatctct
     4381 gccgctctgc taggtagccc gatacgattg atggcggtcc tgggggctat ttgcggaact
     4441 gcgggcgtgg cgctgttggt gttgacacca aacgcagcgc tagatcctgt cggcgtcgca
     4501 gcgggcctgg cgggggcggt ttccatggcg ttcggaaccg tgctgacccg caagtggcaa
     4561 cctcccgtgc ctctgctcac ctttaccgcc tggcaactgg cggccggagg acttctgctc
     4621 gttccagtag ctttagtgtt tgatccgcca atcccgatgc ctacaggaac caatgttctc
     4681 ggcctggcgt ggctcggcct gatcggagcg ggtttaacct acttcctttg gttccggggg
     4741 atctcgcgac tcgaacctac agttgtttcc ttactgggct ttctcagccc cagatctggg
     4801 gtcgatcagc cggggatgca tcaggccgac agtcggaact tcgggtcccc gacctgtacc
     4861 attcggtgag caatggatag gggagttgat atcgtcaacg ttcacttcta aagaaatagc
     4921 gccactcagc ttcctcagcg gctttatcca gcgatttcct attatgtcgg catagttctc
     4981 aagatcgaca gcctgtcacg gttaagcgag aaatgaataa gaaggctgat aattcggatc
     5041 tctgcgaggg agatgatatt tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac
     5101 atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc
     5161 gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc
     5221 gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
     5281 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca
     5341 acttaataac acattgcgga cgtttttaat gtactggggt ggtttttctt ttcaccagtg
     5401 agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt
     5461 ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttccg aaatcggcaa
     5521 aatcccttat aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa
     5581 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     5641 gggcgatggc ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg
     5701 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     5761 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcca ttcaggctgc
     5821 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     5881 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     5941 gtaaaacgac ggccagtgaa ttaattccca tcttgaaaga aatatagttt aaatatttat
     6001 tgataaaata acaagtcagg tattatagtc caagcaaaaa cataaattta ttgatgcaag
     6061 tttaaattca gaaatatttc aataactgat tatatcagct ggtacattgc cgtagatgaa
     6121 agactgagtg cgatattatg tgtaatacat aaattgatga tatagctagc ttagctcatc
     6181 gggggatcga tccgtcgaag ctagcttggg tcccgctcag aagaactcgt caagaaggcg
     6241 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     6301 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     6361 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     6421 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     6481 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     6541 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     6601 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     6661 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     6721 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     6781 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
     6841 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     6901 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     6961 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatcc aagctcccat
     7021 gggccctcga ctagagtcga gatctggatt gagagtgaat atgagactct aattggatac
     7081 cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt gctagctgat
     7141 agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt gcttagctca
     7201 ttaaactcca gaaacccgcg gctgagtggc tccttcaacg ttgcggttct gtcagttcca
     7261 aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc ccttaattct
     7321 ccgctcatga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt
     7381 ttactttgca gggcttccca accttaccag agggcgcccc agctggcaat tccggttcgc
     7441 ttgctgtcca taaaaccgcc cagtctagct atcgccatgt aagcccactg caagctacct
     7501 gctttctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc
     7561 ggggtcagca ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt tagcagccct
     7621 tgcgccctga gtgcttgcgg cagcgtgaag ctttggaacc atcttttggg ttcctttctt
     7681 cttctctttt tttgtttctc tcttaaaagt cttttctctt ttcaagtgct tcaaacaaaa
     7741 ctaatttgtt ataaagggag tttctctatt ttattatata gcaaaaaact tccaaaattt
     7801 ctcatttctg tgtttaacct tttcgtacat cagttttaaa gcacagagtg ctcaatgttc
     7861 ttccaatatc gttattaata aattttgatt aaattcaatc aaatcggagt tatattacca
     7921 catgaggtta aagggccata ttaaaaagtc tgcacttcat atgagcaaca aggcttttat
     7981 gtctttatgg ttgatttgat ggcccatata tgatagttca aaggcccata ttaaaaaatg
     8041 ccctaacatg attcgattcg tatataaaga cgcttaatgt gacaagcgaa cttgctcatt
     8101 agggtttctc atctacgacg gcgtgggcta gaggatccgg cgcgcccggg ctcaggccta
     8161 ttaattaaac tagtatgaga aaaggagaag aacttttcac tggagttgtc ccaattctta
     8221 ttgaattaga tggtgatgtt aatgggcaca aattttttgt ccgtggagag ggtgaaggtg
     8281 atgcaacaat cggaaaactt agccttaaat ttatttgcac tactggaaaa ctacctgttc
     8341 catggccaac acttgtcact acccttacct atggtgttca atgcttttca agatacccag
     8401 atcatatgaa gcggcacgac ttcttcaaga gcgccatgcc tgagggatac gtgcaggaga
     8461 ggaccatcta tttcaaggac gacgggactt acaagacacg tgctgaagtc aagtttgagg
     8521 gagacaccct cgtcaacagg atcgagctta agggaattga tttcaaggag gacggaaaca
     8581 tcctcggcca caagttggaa tacaacttta actcccacaa agtatacatc acggccgaca
     8641 agcaaaataa cggcatcaaa gccaacttca caattcgcca caacgttgaa gacggctctg
     8701 tgcaactcgc tgatcattat caacaaaata ctccaattgg cgatggccct gtccttttac
     8761 cataagagct cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg
     8821 aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat
     8881 gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc
     8941 ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa
     9001 ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tcagatcggc tgagtggctc
     9061 cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg
     9121 ggggtcataa cgtgactccc ttaattctcc gctcatgatc agattgtcgt ttcccgcctt
     9181 cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
     9241 gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt
     9301 gtatgtgcat gccaaccaca gggttcccca gatctggcgc cggccagcga gacgagcaag
     9361 attggccgcc gcccgaaacg atccgacagc gcgcccagca caggtgcgca ggcaaattgc
     9421 accaacgcat acagcgccag cagaatgcca tagtgggcgg tgacgtcgtt cgagtgaacc
     9481 agatcgcgca ggaggcccgg cagcaccggc ataatcaggc cgatgccgac agcgtcgagc
     9541 gcgacagtgc tcagaattac gatcaggggt atgttgggtt tcacgtctgg cctccggacc
     9601 agcctccgct ggtccgattg aacgcgcgga ttctttatca ctgataagtt ggtggacata
     9661 ttatgtttat cagtgataaa gtgtcaagca tgacaaagtt gcagccgaat acagtgatcc
     9721 gtgccgccct ggacctgttg aacgaggtcg gcgtagacgg tctgacgaca cgcaaactgg
     9781 cggaacggtt gggggttcag cagccggcgc tttactggca cttcaggaac aagcgggcgc
     9841 tgctcgacgc actggccgaa gccatgctgg cggagaatca tacgcattcg gtgccgagag
     9901 ccgacgacga ctggcgctca tttctgatcg ggaatgcccg cagcttcagg caggcgctgc
     9961 tcgcctaccg cgatggcgcg cgcatccatg ccggcacgcg accgggcgca ccgcagatgg
    10021 aaacggccga cgcgcagctt cgcttcctct gcgaggcggg tttttcggcc ggggacgccg
    10081 tcaatgcgct gatgacaatc agctacttca ctgttggggc cgtgcttgag gagcaggccg
    10141 gcgacagcga tgccggcgag cgcggcggca ccgttgaaca ggctccgctc tcgccgctgt
    10201 tgcgggccgc gatagacgcc ttcgacgaag ccggtccgga cgcagcgttc gagcagggac
    10261 tcgcggtgat tgtcgatgga ttggcgaaaa ggaggctcgt tgtcaggaac gttgaaggac
    10321 cgagaaaggg tgacgattga tcaggaccgc tgccggagcg caacccactc actacagcag
    10381 agccatgtag acaacatccc ctcccccttt ccaccgcgtc agacgcccgt agcagcccgc
    10441 tacgggcttt ttcatgccct gccctagcgt ccaagcctca cggccgcgct cggcctctct
    10501 ggcggccttc tggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    10561 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    10621 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    10681 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    10741 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    10801 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    10861 ctttctccct tcgggaagcg tggcgctttt ccgctgcata accctgcttc ggggtcatta
    10921 tagcgatttt ttcggtatat ccatcctttt tcgcacgata tacaggattt tgccaaaggg
    10981 ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag
    11041 gcccacccgc gagcgggtgt tccttcttca ctgtccctta ttcgcacctg gcggtgctca
    11101 acgggaatcc tgctctgcga ggctggccgg ctaccgccgg cgtaacagat gagggcaagc
    11161 ggatggctga tgaaaccaag ccaaccagga agggcagccc acctatcaag gtgtactgcc
    11221 ttccagacga acgaagagcg attgaggaaa aggcggcggc ggccggcatg agcctgtcgg
    11281 cctacctgct ggccgtcggc cagggctaca aaatcacggg cgtcgtggac tatgagcacg
    11341 tccgcgagct ggcccgcatc aatggcgacc tgggccgcct gggcggcctg ctgaaactct
    11401 ggctcaccga cgacccgcgc acggcgcggt tcggtgatgc cacgatcctc gccctgctgg
    11461 cgaagatcga agagaagcag gacgagcttg gcaaggtcat gatgggcgtg gtccgcccga
    11521 gggcagagcc atgacttttt tagccgctaa aacggccggg gggtgcgcgt gattgccaag
    11581 cacgtcccca tgcgctccat caagaagagc gacttcgcgg agctggtgaa gtacatcacc
    11641 gacgagcaag gcaagaccga gcgcctttcc gacgctcacc gggctggttg ccctcgccgc
    11701 tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc
    11761 gagacaccgc ggccgccggc gttgtggata cctcgcggaa aacttggccc tcactgacag
    11821 atgaggggcg gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt tgacagatga
    11881 ggggcaggct cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa
    11941 cgcctgattt tacgcgagtt tcccacagat gatgtggaca agcctgggga taagtgccct
    12001 gcggtattga cacttgaggg gcgcgactac tgacagatga ggggcgcgat ccttgacact
    12061 tgaggggcag agtgctgaca gatgaggggc gcacctattg acatttgagg ggctgtccac
    12121 aggcagaaaa tccagcattt gcaagggttt ccgcccgttt ttcggccacc gctaacctgt
    12181 cttttaacct gcttttaaac caatatttat aaaccttgtt tttaaccagg gctgcgccct
    12241 gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc tcccggcccg
    12301 ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc
    12361 caaaaatggc agcgctggca gtccttgcca ttgccgggat cggggcagta acgggatggg
    12421 cgatcagccc gagcgcgacg cccggaagca ttgacgtgcc gcaggtgctg gcatcgacat
    12481 tcagcgacca gg
//
