LOCUS       sequence-209774-       10880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10880)
  AUTHORS   Tan MJ, White EA, Sowa ME, Harper JW, Aster JC, Howley PM
  TITLE     Cutaneous beta-human papillomavirus E6 proteins bind Mastermind-like
            coactivators and repress Notch signaling.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Apr 30.
  PUBMED    22547818
REFERENCE   2  (bases 1 to 10880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Apr 30."
COMMENT     Sequence Label: p6874 pHAGE-N-V5-MAML1-ADDAA
FEATURES             Location/Qualifiers
     source          1..10880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2736..2754
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2785..2809
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2896..2905
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2908..2949
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2965..2989
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6041..6065)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6084..6583
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6119..6142)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6516..6535
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6604..7203
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6604..6623)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7100..7120
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7270..7858
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7323..7343)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7741..7752)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7933..8166
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8285..8303)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8371..8475
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8476..8544,8545..9336)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8694..8713)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9507..10095
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9996..10015
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10249..10266
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10506..10527
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10542..10547,10548..10565,10566..10572)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10580..10596
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10585..10607
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10604..10620
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10604..10620
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10670..10689
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata gagatctccc
     2761 tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg
     2821 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
     2881 agcgtttaaa cgctagccac catggcgggt aagcctatcc ctaaccctct cctcggtctc
     2941 gattctacgg ctcgaggtta tcaaacaagt ttgtacaaaa aagttggcat ggtgctgccc
     3001 acctgcccca tggcggagtt cgcgctgccg cggcacagcg cggtcatgga gcgccttcgc
     3061 cggcgcatcg agctgtgccg gcgccaccac agcacctgcg aggcccgcta cgaggccgtg
     3121 tcgcccgagc gcctggagct ggagcgccaa cacaccttcg ccctgcacca gcgctgcatc
     3181 caggccaagg ccaagcgcgc cgggaagcac aggcagccgc ccgccgccac ggccccggcg
     3241 cccgccgccc cggccccgcg cctggacgcc gctgacggcc ccgagcacgg ccgcccggcc
     3301 acgcatcttc atgatacagt taagaggaat cttgacagcg ccacttcccc tcagaatggc
     3361 gatcaacaga atggctacgg ggacctcttt cctgggcata agaagactcg ccgggaggcc
     3421 cctctgggag ttgccatctc ttccaatgga ctgcctccag cctcccccct cggtcagtct
     3481 gacaagcctt ctggagccga cgccctgcag tccagtggga agcactctct ggggctagac
     3541 tctctcaaca aaaagcgtct ggctgactcc agccttcact tgaatggagg cagtaacccc
     3601 agtgagtcat ttcctctgag cctgaataaa gaactgaagc aggagcctgt cgaagacctg
     3661 ccttgcatga tcactgggac tgtcggctcc atatcgcaaa gcaacctcat gccagacctc
     3721 aaccttaacg agcaggagtg gaaggagctc atcgaggagc tgaacaggtc ggtgcccgat
     3781 gaagacatga aggacctgtt taatgaggac ttcgaggaga agaaggaccc agagtcttct
     3841 ggctctgcca cacaaacccc cttggcacag gacattaata ttaagacgga attctctcca
     3901 gcagcctttg agcaagaaca gttaggctct ccacaagtga gggccgggtc tgcagggcag
     3961 acctttctgg ggccttcctc tgcccctgtg agtacagatt cccccagcct agggggctcc
     4021 caaaccttat tccacacctc tggtcagccc cgggcggaca atcccagtcc aaacctgatg
     4081 ccggcatcag cccaggccca gaacgcacaa agagcccttg caggtgtggt attgcccagt
     4141 cagggcccag gaggggcctc agagctgtcc tctgcccacc agctccagca gatcgctgcc
     4201 aagcagaagc gcgagcagat gctccagaac ccacagcagg ccaccccggc accagccccg
     4261 ggccagatgt ccacatggca gcagacgggg ccctcccaca gttccttaga tgtcccttac
     4321 cccatggaga agcctgccag cccttccagc tacaagcaag acttcactaa ctccaaactg
     4381 ctcatgatgc ctagtgtgaa taagagttcc cctcggcccg gaggccccta cctccagccc
     4441 agccatgtga acctgctgag tcaccagcca ccgagtaact tgaatcagaa ctccgcgaat
     4501 aaccaggggt ctgtgctgga ctacggcaat acaaaacccc tttctcatta caaagcggac
     4561 tgtgggcaag gcagcccggg gtctggccag agcaagccag ccctgatggc ttatcttccc
     4621 cagcagctgt cccatataag tcacgagcag aactccctgt ttctgatgaa gccaaagcca
     4681 ggaaatatgc ctttccgatc actggttcca cctggccagg agcagaaccc ttccagtgtc
     4741 cctgtgcaag cccaggctac cagtgttggg acccagccgc ctgccgtgtc cgtggccagc
     4801 tcccacaaca gctcccccta tctcagcagc cagcaacagg ccgctgtaat gaagcagcat
     4861 cagttgcttt tggaccaaca gaaacaaagg gagcagcagc aaaagcattt acagcaacag
     4921 cagttccttc agaggcaaca gcaccttctc gcggaacagg agaagcaaca gtttcagcgc
     4981 catctgaccc gcccaccacc ccagtaccaa gacccgacac aaggcagctt cccacagcag
     5041 gttggacagt tcacagggtc ctctgctgcc gtgcccggca tgaacacctt gggtccatcc
     5101 aactccagct gtcctcgagt gttccctcag gctgggaatc tgatgccaat gggccctgga
     5161 catgcttcag tttcctctct ccccacaaac tcaggccaac aggaccgggg tgtggctcag
     5221 ttccctggct cccaaaacat gcctcagagc agcctctatg gcatggcttc tggcataacc
     5281 cagatagttg cccagccccc gccacaggcc accaatggac atgcccacat tccacggcag
     5341 accaacgtgg gccagaacac ctccgtctca gctgcctatg ggcagaactc tctgggaagc
     5401 tctggcctct cccagcagca caataagggg accctgaacc ctggtttaac aaagccaccg
     5461 gtcccaaggg tgtcaccagc catgggaggc cagaattcct cctggcagca tcagggaatg
     5521 ccgaacctca gtggccagac cccagggaac agcaacgtga gtcccttcac tgcagcctcc
     5581 agtttccaca tgcagcagca ggcccacctg aaaatgtcta gcccgcaatt ctcccaggca
     5641 gtgcccaaca ggcccatggc tcccatgagc tcagcagctg ccgtggggtc cttgctaccc
     5701 ccagtgagtg cacagcagag gaccagcgcc cctgccccag caccaccccc aacagcccct
     5761 cagcagggct tgcctggcct gagcccagca gggcctgagc tgggggcctt cagccagagc
     5821 cctgcctcac agatgggcgg tcgggcgggg ctgcactgca cccaggccta ccctgtgcgg
     5881 accgcgggcc aggagctgcc ttttgcctat agcgggcagc caggtggcag tgggctctct
     5941 agtgtggctg gacacaccga tctgatcgac tccctgctga agaacaggac ttcagaggag
     6001 tggatgagtg atgcggacga cgcggcaggg tctcagtaat acccaacttt cttgtacaaa
     6061 gtggttcgat aacgaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     6121 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
     6181 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
     6241 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
     6301 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
     6361 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
     6421 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
     6481 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
     6541 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt
     6601 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     6661 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     6721 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     6781 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     6841 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     6901 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     6961 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     7021 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     7081 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     7141 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     7201 tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg
     7261 atagatccta atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     7321 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     7381 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     7441 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     7501 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     7561 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     7621 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     7681 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     7741 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     7801 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     7861 gagatccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     7921 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     7981 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     8041 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     8101 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     8161 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     8221 tatcagagag tgagaggccc gggttaatta aggaaagggc tagatcattc ttgaagacga
     8281 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     8341 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     8401 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     8461 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     8521 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     8581 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     8641 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     8701 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     8761 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     8821 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     8881 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     8941 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     9001 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     9061 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     9121 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     9181 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     9241 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     9301 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     9361 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     9421 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9481 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     9541 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     9601 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     9661 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9721 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9781 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9841 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9901 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9961 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    10021 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    10081 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    10141 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    10201 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    10261 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
    10321 cattaatgca gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg
    10381 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
    10441 gcaaaaagct ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    10501 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
    10561 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
    10621 atgattacga atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    10681 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
    10741 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta
    10801 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
    10861 tgtactacaa acttagtagt
//
